EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-Electron Microscopy Structures of Yeast Alcohol Dehydrogenase.
ジャーナル・号・ページ	Biochemistry, Vol. 60, Issue 9, Page 663-677, Year 2021
掲載日	2021年3月9日
著者	Sai Rohit Guntupalli / Zhuang Li / Leifu Chang / Bryce V Plapp / Ramaswamy Subramanian /
PubMed 要旨	Structures of yeast alcohol dehydrogenase determined by X-ray crystallography show that the subunits have two different conformational states in each of the two dimers that form the tetramer. ...Structures of yeast alcohol dehydrogenase determined by X-ray crystallography show that the subunits have two different conformational states in each of the two dimers that form the tetramer. Apoenzyme and holoenzyme complexes relevant to the catalytic mechanism were described, but the asymmetry led to questions about the cooperativity of the subunits in catalysis. This study used cryo-electron microscopy (cryo-EM) to provide structures for the apoenzyme, two different binary complexes with NADH, and a ternary complex with NAD and 2,2,2-trifluoroethanol. All four subunits in each of these complexes are identical, as the tetramers have 2 symmetry, suggesting that there is no preexisting asymmetry and that the subunits can be independently active. The apoenzyme and one enzyme-NADH complex have "open" conformations and the inverted coordination of the catalytic zinc with Cys-43, His-66, Glu-67, and Cys-153, whereas another enzyme-NADH complex and the ternary complex have closed conformations with the classical coordination of the zinc with Cys-43, His-66, Cys-153, and a water or the oxygen of trifluoroethanol. The conformational change involves interactions of Arg-340 with the pyrophosphate group of the coenzyme and Glu-67. The cryo-EM and X-ray crystallography studies provide structures relevant for the catalytic mechanism.
リンク	Biochemistry / PubMed:33620215
手法	EM (単粒子)
解像度	2.67 - 3.2 Å
構造データ	22805 7kc2 EMDB-22805, PDB-7kc2: Symmetry in Yeast Alcohol Dehydrogenase 1 -Closed Form with NADH 手法: EM (単粒子) / 解像度: 2.67 Å 22807 7kcb EMDB-22807, PDB-7kcb: Symmetry in Yeast Alcohol Dehydrogenase 1 -Closed Form with NAD+ and Trifluoroethanol 手法: EM (単粒子) / 解像度: 2.77 Å 22817 7kcq EMDB-22817, PDB-7kcq: Symmetry in Yeast Alcohol Dehydrogenase 1 -Open Form of Apoenzyme 手法: EM (単粒子) / 解像度: 3.2 Å 22902 7kjy EMDB-22902, PDB-7kjy: Symmetry in Yeast Alcohol Dehydrogenase 1 - Open Form with NADH 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM / ニコチンアミドアデニンジヌクレオチド ChemComp-HOH: WATER / 水 ChemComp-ETF*: TRIFLUOROETHANOL / 2,2,2-トリフルオロエタノ-ル / 2,2,2-トリフルオロエタノール
由来	saccharomyces cerevisiae (パン酵母) Baker's yeast (パン酵母)
キーワード	OXIDOREDUCTASE (酸化還元酵素) / Alcohol dehydrogenase (アルコールデヒドロゲナーゼ) / NADH complex (ニコチンアミドアデニンジヌクレオチド) / holo-enzyme complex / holo-enzymem complex / NADH (ニコチンアミドアデニンジヌクレオチド)