EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into G protein activation by D1 dopamine receptor.
ジャーナル・号・ページ	Sci Adv, Vol. 8, Issue 23, Page eabo4158, Year 2022
掲載日	2022年6月10日
著者	Xiao Teng / Sijia Chen / Qing Wang / Zhao Chen / Xiaoying Wang / Niu Huang / Sanduo Zheng /
PubMed 要旨	G protein-coupled receptors (GPCRs) comprise the largest family of membrane receptors and are the most important drug targets. An agonist-bound GPCR engages heterotrimeric G proteins and triggers the ...G protein-coupled receptors (GPCRs) comprise the largest family of membrane receptors and are the most important drug targets. An agonist-bound GPCR engages heterotrimeric G proteins and triggers the exchange of guanosine diphosphate (GDP) with guanosine triphosphate (GTP) to promote G protein activation. A complete understanding of molecular mechanisms of G protein activation has been hindered by a lack of structural information of GPCR-G protein complex in nucleotide-bound states. Here, we report the cryo-EM structures of the D1 dopamine receptor and mini-G complex in the nucleotide-free and nucleotide-bound states. These structures reveal major conformational changes in Gα such as structural rearrangements of the carboxyl- and amino-terminal α helices that account for the release of GDP and the GTP-dependent dissociation of Gα from Gβγ subunits. As validated by biochemical and cellular signaling studies, our structures shed light into the molecular basis of the entire signaling events of GPCR-mediated G protein activation.
リンク	Sci Adv / PubMed:35687690 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.16 Å
構造データ	31404 7f0t EMDB-31404, PDB-7f0t: Cryo-EM structure of dopamine receptor 1 and mini-Gs complex with dopamine bound 手法: EM (単粒子) / 解像度: 3.1 Å 31421 7f1o EMDB-31421, PDB-7f1o: Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex with Nb35 手法: EM (単粒子) / 解像度: 3.13 Å 31425 7f1z EMDB-31425, PDB-7f1z: Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex without Nb35 手法: EM (単粒子) / 解像度: 3.46 Å 31426 7f23 EMDB-31426, PDB-7f23: Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex with Nb35 手法: EM (単粒子) / 解像度: 3.58 Å 31427 7f24 EMDB-31427, PDB-7f24: Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex without Nb35 手法: EM (単粒子) / 解像度: 4.16 Å
化合物	ChemComp-LDP: L-DOPAMINE / ド-パミン / 薬剤YM / Dopamine (medication) ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM / グアノシン三リン酸
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / GPCR (Gタンパク質共役受容体) / dopamine receptor (ドーパミン受容体) / mini-Gs