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-Structure paper
タイトル | High-resolution cryo-EM structure of photosystem II reveals damage from high-dose electron beams. |
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ジャーナル・号・ページ | Commun Biol, Vol. 4, Issue 1, Page 382, Year 2021 |
掲載日 | 2021年3月22日 |
著者 | Koji Kato / Naoyuki Miyazaki / Tasuku Hamaguchi / Yoshiki Nakajima / Fusamichi Akita / Koji Yonekura / Jian-Ren Shen / |
PubMed 要旨 | Photosystem II (PSII) plays a key role in water-splitting and oxygen evolution. X-ray crystallography has revealed its atomic structure and some intermediate structures. However, these structures are ...Photosystem II (PSII) plays a key role in water-splitting and oxygen evolution. X-ray crystallography has revealed its atomic structure and some intermediate structures. However, these structures are in the crystalline state and its final state structure has not been solved. Here we analyzed the structure of PSII in solution at 1.95 Å resolution by single-particle cryo-electron microscopy (cryo-EM). The structure obtained is similar to the crystal structure, but a PsbY subunit was visible in the cryo-EM structure, indicating that it represents its physiological state more closely. Electron beam damage was observed at a high-dose in the regions that were easily affected by redox states, and reducing the beam dosage by reducing frames from 50 to 2 yielded a similar resolution but reduced the damage remarkably. This study will serve as a good indicator for determining damage-free cryo-EM structures of not only PSII but also all biological samples, especially redox-active metalloproteins. |
リンク | Commun Biol / PubMed:33753866 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 1.95 - 2.22 Å |
構造データ | EMDB-30547, PDB-7d1t: EMDB-30548, PDB-7d1u: EMDB-30549: EMDB-30550: |
化合物 | ChemComp-OEX: ChemComp-FE2: ChemComp-CL: ChemComp-CLA: ChemComp-PHO: ChemComp-BCR: ChemComp-SQD: ChemComp-PL9:
ChemComp-UNL: ChemComp-LMG: ChemComp-DGD: ChemComp-LMT: ChemComp-BCT: ChemComp-LHG: ChemComp-HEM: ChemComp-MG: ChemComp-HEC: ChemComp-RRX: ChemComp-HOH: |
由来 |
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キーワード | PHOTOSYNTHESIS / Photosystem II |