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-Structure paper
タイトル | Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly. |
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ジャーナル・号・ページ | Sci Adv, Vol. 7, Issue 13, Year 2021 |
掲載日 | 2021年3月24日 |
著者 | Natalya Leneva / Oleksiy Kovtun / Dustin R Morado / John A G Briggs / David J Owen / |
PubMed 要旨 | Retromer is a master regulator of cargo retrieval from endosomes, which is critical for many cellular processes including signaling, immunity, neuroprotection, and virus infection. The retromer core ...Retromer is a master regulator of cargo retrieval from endosomes, which is critical for many cellular processes including signaling, immunity, neuroprotection, and virus infection. The retromer core (VPS26/VPS29/VPS35) is present on cargo-transporting, tubular carriers along with a range of sorting nexins. Here, we elucidate the structural basis of membrane tubulation and coupled cargo recognition by metazoan and fungal retromer coats assembled with the non-Bin1/Amphiphysin/Rvs (BAR) sorting nexin SNX3 using cryo-electron tomography. The retromer core retains its arched, scaffolding structure but changes its mode of membrane recruitment when assembled with different SNX adaptors, allowing cargo recognition at subunit interfaces. Thus, membrane bending and cargo incorporation can be modulated to allow retromer to traffic cargoes along different cellular transport routes. |
リンク | Sci Adv / PubMed:33762348 / PubMed Central |
手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 8.9 - 9.5 Å |
構造データ | EMDB-12220: VPS35/VPS29 arch of metazoan membrane-assembled retromer:SNX3 complex EMDB-12221: VPS26 dimer region of metazoan membrane-assembled retromer:SNX3 complex EMDB-12222: EMDB-12223, PDB-7blp: EMDB-12224, PDB-7blq: EMDB-12225: EMDB-12226, PDB-7blr: |
化合物 | ChemComp-PIB: |
由来 |
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キーワード | ENDOCYTOSIS / endosomes / coat proteins / membrane trafficking / cargo-sorting |