EMDB/PDB/SASBDBから引用されている文献のデータベース

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構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
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IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1.
ジャーナル・号・ページ	EMBO J, Vol. 40, Issue 12, Page e107608, Year 2021
掲載日	2021年6月15日
著者	Antonio Galindo / Vicente J Planelles-Herrero / Gianluca Degliesposti / Sean Munro /
PubMed 要旨	The TRAPP complexes are nucleotide exchange factors that play essential roles in membrane traffic and autophagy. TRAPPII activates Rab11, and TRAPPIII activates Rab1, with the two complexes sharing a ...The TRAPP complexes are nucleotide exchange factors that play essential roles in membrane traffic and autophagy. TRAPPII activates Rab11, and TRAPPIII activates Rab1, with the two complexes sharing a core of small subunits that affect nucleotide exchange but being distinguished by specific large subunits that are essential for activity in vivo. Crystal structures of core subunits have revealed the mechanism of Rab activation, but how the core and the large subunits assemble to form the complexes is unknown. We report a cryo-EM structure of the entire Drosophila TRAPPIII complex. The TRAPPIII-specific subunits TRAPPC8 and TRAPPC11 hold the catalytic core like a pair of tongs, with TRAPPC12 and TRAPPC13 positioned at the joint between them. TRAPPC2 and TRAPPC2L link the core to the two large arms, with the interfaces containing residues affected by disease-causing mutations. The TRAPPC8 arm is positioned such that it would contact Rab1 that is bound to the core, indicating how the arm could determine the specificity of the complex. A lower resolution structure of TRAPPII shows a similar architecture and suggests that the TRAPP complexes evolved from a single ur-TRAPP.
リンク	EMBO J / PubMed:34018214 / PubMed Central
手法	EM (単粒子)
解像度	3.6 - 15.0 Å
構造データ	12052 7b6d EMDB-12052, PDB-7b6d: Drosophila melanogaster TRAPPCore (C1, C2, C2L, C3a/b, C4, C5, C6 subunits) 手法: EM (単粒子) / 解像度: 4.27 Å 12053 7b6e EMDB-12053: Drosophila melanogaster TRAPP C8, C12 and C13 region map from the TRAPPIII complex PDB-7b6e: Drosophila melanogaster TRAPP C8 subunits region 355 to 661 手法: EM (単粒子) / 解像度: 4.5 Å 12054 7b6h EMDB-12054: TRAPP C11 region of the whole TRAPPIII complex PDB-7b6h: Drosophila melanogaster TRAPP C11 subunits region 1 to 718 手法: EM (単粒子) / 解像度: 5.4 Å 12056 7b6r EMDB-12056: Drosophila melanogaster TRAPPIII complex PDB-7b6r: Drosophila melanogaster TRAPPIII partial complex: core plus C8 and C11 attached region 手法: EM (単粒子) / 解像度: 5.8 Å 12057 7b6x EMDB-12057, PDB-7b6x: TRAPPCore from the MiniTRAPPIII complex 手法: EM (単粒子) / 解像度: 3.6 Å 12058 7b6y EMDB-12058: C8 region from the MiniTRAPPIII complex PDB-7b6y: C8(355-600) from the MiniTRAPPIII complex 手法: EM (単粒子) / 解像度: 5.7 Å 12060 7b6z EMDB-12060: TRAPPC11 region from the MiniTRAPPIII complex PDB-7b6z: TRAPPC11 subunit (1-716) 手法: EM (単粒子) / 解像度: 5.5 Å 12063 7b70 EMDB-12063: Whole MiniTRAPPIII PDB-7b70: TRAPPCore plus C8 (355-596) and C11 (1-718) from MiniTRAPPIII 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-12066: Drosophila melnaogaster TRAPPII 手法: EM (単粒子) / 解像度: 15.0 Å
由来	drosophila melanogaster (キイロショウジョウバエ)
キーワード	EXOCYTOSIS / Golgi / GEFS / Rab1 / TRAPP / TRAPPIII complex / TRAPP complexes