EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Actinobacteria challenge the paradigm: A unique protein architecture for a well-known, central metabolic complex.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 48, Year 2021
掲載日	2021年11月30日
著者	Eduardo M Bruch / Pierre Vilela / Lu Yang / Alexandra Boyko / Norik Lexa-Sapart / Bertrand Raynal / Pedro M Alzari / Marco Bellinzoni /
PubMed 要旨	α-oxoacid dehydrogenase complexes are large, tripartite enzymatic machineries carrying out key reactions in central metabolism. Extremely conserved across the tree of life, they have been, so far, ...α-oxoacid dehydrogenase complexes are large, tripartite enzymatic machineries carrying out key reactions in central metabolism. Extremely conserved across the tree of life, they have been, so far, all considered to be structured around a high-molecular weight hollow core, consisting of up to 60 subunits of the acyltransferase component. We provide here evidence that Actinobacteria break the rule by possessing an acetyltranferase component reduced to its minimally active, trimeric unit, characterized by a unique C-terminal helix bearing an actinobacterial specific insertion that precludes larger protein oligomerization. This particular feature, together with the presence of an gene coding for both the decarboxylase and the acyltransferase domains on the same polypetide, is spread over Actinobacteria and reflects the association of PDH and ODH into a single physical complex. Considering the central role of the pyruvate and 2-oxoglutarate nodes in central metabolism, our findings pave the way to both therapeutic and metabolic engineering applications.
リンク	Proc Natl Acad Sci U S A / PubMed:34819376 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.35 - 3.92 Å
構造データ	EMDB-11600: Cubic core of the dihydrolipoamide acyltransferase (E2b) component of the branched-chain alpha-ketoacid dehydrogenase complex (BCKDH) from M. tuberculosis 手法: EM (単粒子) / 解像度: 3.92 Å PDB-6zzi: Crystal structure of the catalyic domain of Corynebacterium glutamicum acetyltransferase AceF (E2p). 手法: X-RAY DIFFRACTION / 解像度: 1.932 Å PDB-6zzj: Crystal structure of the catalytic domain of Corynebacterium glutamicum acetyltransferase AceF (E2p) in complex with oxidized CoA. 手法: X-RAY DIFFRACTION / 解像度: 1.35 Å PDB-6zzk: Crystal structure of the catalytic domain of C. glutamicum AceF (E2p) in ternary complex with CoA and dihydrolipoamide. 手法: X-RAY DIFFRACTION / 解像度: 2.09 Å PDB-6zzl: Crystal structure of the catalytic domain plus N-terminal linker of the acetyltransferase AceF (E2p) from Corynebacterium glutamicum. 手法: X-RAY DIFFRACTION / 解像度: 2.229 Å PDB-6zzm: Crystal structure of the catalytic domain of Corynebacterium mustelae predicted acetyltransferase AceF (E2p). 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-6zzn: Crystal structure of the cubic catalytic core of the Mycobacterium tuberculosis branched-chain alphaketoacid acyltransferase component (E2b). 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å
化合物	ChemComp-HOH: WATER ChemComp-CAO: OXIDIZED COENZYME A ChemComp-EPE: 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES / pH緩衝剤YM ChemComp-COA: COENZYME A / CoA ChemComp-LPM: 6,8-DIMERCAPTO-OCTANOIC ACID AMIDE ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-ACT: ACETATE ION / 酢酸イオン ChemComp-IMD*: IMIDAZOLE / 1H-イミダゾ-ル-3-カチオン
由来	mycobacterium tuberculosis h37rv (結核菌) corynebacterium glutamicum (strain atcc 13032 / dsm 20300 / jcm 1318 / lmg 3730 / ncimb 10025) (バクテリア) corynebacterium glutamicum atcc 13032 (バクテリア) corynebacterium mustelae (バクテリア)
キーワード	TRANSFERASE / PDH / ODH / acetyltransferase / lipoamide / corynebacterium / CoA / BCKDH / acyltransferase / mycobacterium / tuberculosis