EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ.
ジャーナル・号・ページ	Science, Vol. 371, Issue 6524, Year 2021
掲載日	2021年1月1日
著者	Nelly Said / Tarek Hilal / Nicholas D Sunday / Ajay Khatri / Jörg Bürger / Thorsten Mielke / Georgiy A Belogurov / Bernhard Loll / Ranjan Sen / Irina Artsimovitch / Markus C Wahl /
PubMed 要旨	Factor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo-electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ...Factor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo-electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ρ on a pathway to terminating NusA/NusG-modified elongation complexes. An open ρ ring contacts NusA, NusG, and multiple regions of RNA polymerase, trapping and locally unwinding proximal upstream DNA. NusA wedges into the ρ ring, initially sequestering RNA. Upon deflection of distal upstream DNA over the RNA polymerase zinc-binding domain, NusA rotates underneath one capping ρ subunit, which subsequently captures RNA. After detachment of NusG and clamp opening, RNA polymerase loses its grip on the RNA:DNA hybrid and is inactivated. Our structural and functional analyses suggest that ρ, and other termination factors across life, may use analogous strategies to allosterically trap transcription complexes in a moribund state.
リンク	Science / PubMed:33243850 / PubMed Central
手法	EM (単粒子)
解像度	3.9 - 5.7 Å
構造データ	11087 6z9p EMDB-11087: Transcription termination complex 1 PDB-6z9p: Transcription termination intermediate complex 1 手法: EM (単粒子) / 解像度: 3.9 Å 11088 6z9q EMDB-11088, PDB-6z9q: Transcription termination intermediate complex 2 手法: EM (単粒子) / 解像度: 5.7 Å 11089 6z9r EMDB-11089, PDB-6z9r: Transcription termination intermediate complex 3 手法: EM (単粒子) / 解像度: 4.1 Å 11090 6z9s EMDB-11090: Transcription termination interdmediate complex 4 PDB-6z9s: Transcription termination intermediate complex 4 手法: EM (単粒子) / 解像度: 4.4 Å 11091 6z9t EMDB-11091, PDB-6z9t: Transcription termination intermediate complex 5 手法: EM (単粒子) / 解像度: 4.1 Å 11722 7adb EMDB-11722, PDB-7adb: Transcription termination intermediate complex 1 delta NusG 手法: EM (単粒子) / 解像度: 4.4 Å 11723 7adc EMDB-11723, PDB-7adc: Transcription termination intermediate complex 3 delta NusG 手法: EM (単粒子) / 解像度: 4.0 Å 11724 7add EMDB-11724, PDB-7add: Transcription termination intermediate complex IIIa 手法: EM (単粒子) / 解像度: 4.3 Å 11725 7ade EMDB-11725, PDB-7ade: Transcription termination complex IVa 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-DG: 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE / dGMP / dGMPYM / デオキシグアノシン一リン酸 ChemComp-HOH: WATER / 水 ChemComp-DT: THYMIDINE-5'-MONOPHOSPHATE / TMP / dTMPYM / チミジル酸 ChemComp-DA: 2'-DEOXYADENOSINE-5'-MONOPHOSPHATE / dAMP / dAMPYM / デオキシアデノシン一リン酸
由来	escherichia coli (大腸菌) synthetic construct (人工物)
キーワード	TRANSCRIPTION (転写 (生物学)) / Termination / Helicase (ヘリカーゼ) / RNA Polymerase (RNAポリメラーゼ) / Rho