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-Structure paper
タイトル | Type III ATP synthase is a symmetry-deviated dimer that induces membrane curvature through tetramerization. |
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ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 5342, Year 2020 |
掲載日 | 2020年10月22日 |
著者 | Rasmus Kock Flygaard / Alexander Mühleip / Victor Tobiasson / Alexey Amunts / |
PubMed 要旨 | Mitochondrial ATP synthases form functional homodimers to induce cristae curvature that is a universal property of mitochondria. To expand on the understanding of this fundamental phenomenon, we ...Mitochondrial ATP synthases form functional homodimers to induce cristae curvature that is a universal property of mitochondria. To expand on the understanding of this fundamental phenomenon, we characterized the unique type III mitochondrial ATP synthase in its dimeric and tetrameric form. The cryo-EM structure of a ciliate ATP synthase dimer reveals an unusual U-shaped assembly of 81 proteins, including a substoichiometrically bound ATPTT2, 40 lipids, and co-factors NAD and CoQ. A single copy of subunit ATPTT2 functions as a membrane anchor for the dimeric inhibitor IF. Type III specific linker proteins stably tie the ATP synthase monomers in parallel to each other. The intricate dimer architecture is scaffolded by an extended subunit-a that provides a template for both intra- and inter-dimer interactions. The latter results in the formation of tetramer assemblies, the membrane part of which we determined to 3.1 Å resolution. The structure of the type III ATP synthase tetramer and its associated lipids suggests that it is the intact unit propagating the membrane curvature. |
リンク | Nat Commun / PubMed:33093501 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.5 - 3.1 Å |
構造データ | EMDB-10857, PDB-6ynv: EMDB-10858, PDB-6ynw: EMDB-10859, PDB-6ynx: EMDB-10860: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo dimer EMDB-10861: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo tetramer EMDB-10862, PDB-6yo0: |
化合物 | ChemComp-NAD: ChemComp-CDL: ChemComp-PC1: ChemComp-PO4: ChemComp-UQ8: ChemComp-ATP: ChemComp-MG: ChemComp-PEE: ChemComp-ADP: |
由来 |
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キーワード | MEMBRANE PROTEIN / mitochondria / ATP synthase / oxidoreductase / NAD / central stalk / c-ring / Fo-subcomplex / IF1 dimer / F1Fo dimer / F1Fo tetramer / F1-subcomplex / peripheral stalk / IF1 |