EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Key role of quinone in the mechanism of respiratory complex I.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 4135, Year 2020
掲載日	2020年8月18日
著者	Javier Gutiérrez-Fernández / Karol Kaszuba / Gurdeep S Minhas / Rozbeh Baradaran / Margherita Tambalo / David T Gallagher / Leonid A Sazanov /
PubMed 要旨	Complex I is the first and the largest enzyme of respiratory chains in bacteria and mitochondria. The mechanism which couples spatially separated transfer of electrons to proton translocation in ...Complex I is the first and the largest enzyme of respiratory chains in bacteria and mitochondria. The mechanism which couples spatially separated transfer of electrons to proton translocation in complex I is not known. Here we report five crystal structures of T. thermophilus enzyme in complex with NADH or quinone-like compounds. We also determined cryo-EM structures of major and minor native states of the complex, differing in the position of the peripheral arm. Crystal structures show that binding of quinone-like compounds (but not of NADH) leads to a related global conformational change, accompanied by local re-arrangements propagating from the quinone site to the nearest proton channel. Normal mode and molecular dynamics analyses indicate that these are likely to represent the first steps in the proton translocation mechanism. Our results suggest that quinone binding and chemistry play a key role in the coupling mechanism of complex I.
リンク	Nat Commun / PubMed:32811817 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.109 - 6.1 Å
構造データ	11231 6ziy EMDB-11231, PDB-6ziy: Respiratory complex I from Thermus thermophilus, NADH dataset, major state 手法: EM (単粒子) / 解像度: 4.25 Å 11235 6zjl EMDB-11235, PDB-6zjl: Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state 手法: EM (単粒子) / 解像度: 4.3 Å 11237 6zjn EMDB-11237, PDB-6zjn: Respiratory complex I from Thermus thermophilus, NADH dataset, minor state 手法: EM (単粒子) / 解像度: 6.1 Å 11238 6zjy EMDB-11238, PDB-6zjy: Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state 手法: EM (単粒子) / 解像度: 5.5 Å PDB-6i0d: Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone 手法: X-RAY DIFFRACTION / 解像度: 3.6 Å PDB-6i1p: Respiratory complex I from Thermus thermophilus with bound NADH 手法: X-RAY DIFFRACTION / 解像度: 3.207 Å PDB-6q8o: Respiratory complex I from Thermus thermophilus with bound Piericidin A 手法: X-RAY DIFFRACTION / 解像度: 3.605 Å PDB-6q8w: Respiratory complex I from Thermus thermophilus with bound Aureothin. 手法: X-RAY DIFFRACTION / 解像度: 3.4 Å PDB-6q8x: Respiratory complex I from Thermus thermophilus with bound Pyridaben. 手法: X-RAY DIFFRACTION / 解像度: 3.508 Å PDB-6y11: Respiratory complex I from Thermus thermophilus 手法: X-RAY DIFFRACTION / 解像度: 3.109 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-DCQ: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione / デシルユビキノン ChemComp-NAI: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / NADH ChemComp-HQH: Piericidin A / ピエリシジンA / 抗生剤YM ChemComp-HQW: Aureothin / antitumor, 抗真菌剤, 抗生剤YM ChemComp-HQK: Pyridaben / ピリダベン
由来	thermus thermophilus (バクテリア) thermus thermophilus hb8 (バクテリア) thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (バクテリア)
キーワード	MEMBRANE PROTEIN / Respiratory chain / complex I / NADH:ubiquinone oxidoreductase / electron transfer / proton translocation