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-Structure paper
タイトル | Structural insights into the Ca-dependent gating of the human mitochondrial calcium uniporter. |
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ジャーナル・号・ページ | Elife, Vol. 9, Year 2020 |
掲載日 | 2020年8月7日 |
著者 | Yan Wang / Yan Han / Ji She / Nam X Nguyen / Vamsi K Mootha / Xiao-Chen Bai / Youxing Jiang / |
PubMed 要旨 | Mitochondrial Ca uptake is mediated by an inner mitochondrial membrane protein called the mitochondrial calcium uniporter. In humans, the uniporter functions as a holocomplex consisting of MCU, EMRE, ...Mitochondrial Ca uptake is mediated by an inner mitochondrial membrane protein called the mitochondrial calcium uniporter. In humans, the uniporter functions as a holocomplex consisting of MCU, EMRE, MICU1 and MICU2, among which MCU and EMRE form a subcomplex and function as the conductive channel while MICU1 and MICU2 are EF-hand proteins that regulate the channel activity in a Ca-dependent manner. Here, we present the EM structures of the human mitochondrial calcium uniporter holocomplex (uniplex) in the presence and absence of Ca, revealing distinct Ca dependent assembly of the uniplex. Our structural observations suggest that Ca changes the dimerization interaction between MICU1 and MICU2, which in turn determines how the MICU1-MICU2 subcomplex interacts with the MCU-EMRE channel and, consequently, changes the distribution of the uniplex assemblies between the blocked and unblocked states. |
リンク | Elife / PubMed:32762847 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.17 - 7.0 Å |
構造データ | EMDB-22213: EMDB-22214: EMDB-22215, PDB-6xjv: EMDB-22216, PDB-6xjx: |
由来 |
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キーワード | TRANSPORT PROTEIN (運搬体タンパク質) / ion channel (イオンチャネル) |