EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM study of an archaeal 30S initiation complex gives insights into evolution of translation initiation.
ジャーナル・号・ページ	Commun Biol, Vol. 3, Issue 1, Page 58, Year 2020
掲載日	2020年2月6日
著者	Pierre-Damien Coureux / Christine Lazennec-Schurdevin / Sophie Bourcier / Yves Mechulam / Emmanuelle Schmitt /
PubMed 要旨	Archaeal translation initiation occurs within a macromolecular complex containing the small ribosomal subunit (30S) bound to mRNA, initiation factors aIF1, aIF1A and the ternary complex aIF2:GDPNP: ...Archaeal translation initiation occurs within a macromolecular complex containing the small ribosomal subunit (30S) bound to mRNA, initiation factors aIF1, aIF1A and the ternary complex aIF2:GDPNP:Met-tRNA. Here, we determine the cryo-EM structure of a 30S:mRNA:aIF1A:aIF2:GTP:Met-tRNA complex from Pyrococcus abyssi at 3.2 Å resolution. It highlights archaeal features in ribosomal proteins and rRNA modifications. We find an aS21 protein, at the location of eS21 in eukaryotic ribosomes. Moreover, we identify an N-terminal extension of archaeal eL41 contacting the P site. We characterize 34 N-acetylcytidines distributed throughout 16S rRNA, likely contributing to hyperthermostability. Without aIF1, the 30S head is stabilized and initiator tRNA is tightly bound to the P site. A network of interactions involving tRNA, mRNA, rRNA modified nucleotides and C-terminal tails of uS9, uS13 and uS19 is observed. Universal features and domain-specific idiosyncrasies of translation initiation are discussed in light of ribosomal structures from representatives of each domain of life.
リンク	Commun Biol / PubMed:32029867 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.2 Å
構造データ	10320 6sw9 EMDB-10320: IC2A cryo-EM map of a full archaeal ribosomal translation initiation complex devoid of aIF1 in P. abyssi PDB-6sw9: IC2A model of cryo-EM structure of a full archaeal ribosomal translation initiation complex devoid of aIF1 in P. abyssi 手法: EM (単粒子) / 解像度: 4.2 Å 10322 6swc EMDB-10322: IC2B cryo-EM structure of a full archaeal ribosomal translation initiation complex devoid of aIF1 in P. abyssi PDB-6swc: IC2B model of cryo-EM structure of a full archaeal ribosomal translation initiation complex devoid of aIF1 in P. abyssi 手法: EM (単粒子) / 解像度: 3.3 Å 10323 6swd EMDB-10323: IC2 body cryo-EM structure of a full archaeal ribosomal translation initiation complex devoid of aIF1 in P. abyssi PDB-6swd: IC2 body model of cryo-EM structure of a full archaeal ribosomal translation initiation complex devoid of aIF1 in P. abyssi 手法: EM (単粒子) / 解像度: 3.2 Å 10324 6swe EMDB-10324, PDB-6swe: IC2 head of cryo-EM structure of a full archaeal ribosomal translation initiation complex devoid of aIF1 in P. abyssi 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-MET: METHIONINE / メチオニン / メチオニン ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体YM / 5'-Guanylyl imidodiphosphate ChemComp-HOH*: WATER / 水
由来	pyrococcus abyssi ge5 (古細菌) escherichia coli (大腸菌) Saccharolobus solfataricus P2 (古細菌) pyrococcus abyssi (strain ge5 / orsay) (古細菌) saccharolobus solfataricus (strain atcc 35092 / dsm 1617 / jcm 11322 / p2) (古細菌)
キーワード	RIBOSOME (リボソーム) / Translation initiation / cryo-EM (低温電子顕微鏡法) / tRNA (転移RNA) / evolution (進化) / archaea (古細菌) / rRNA modifications