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-Structure paper
タイトル | Molecular insight into RNA polymerase I promoter recognition and promoter melting. |
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ジャーナル・号・ページ | Nat Commun, Vol. 10, Issue 1, Page 5543, Year 2019 |
掲載日 | 2019年12月5日 |
著者 | Yashar Sadian / Florence Baudin / Lucas Tafur / Brice Murciano / Rene Wetzel / Felix Weis / Christoph W Müller / |
PubMed 要旨 | RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I ...RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III. |
リンク | Nat Commun / PubMed:31804486 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.7 - 4.0 Å |
構造データ | EMDB-10006, PDB-6rui: EMDB-10007, PDB-6ruo: EMDB-10019: EMDB-10020: EMDB-10038, PDB-6rwe: |
化合物 | ChemComp-ZN: |
由来 |
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キーワード | TRANSCRIPTION / RNA Polymerase I / Pre-initiation complex / PIC / Closed Complex / CC / Core Factor / CF / Rrn3 / POL1 / tandem winged helix / tWH / Promoter escape / Open Complex / OC / DNA opening |