EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein.
ジャーナル・号・ページ	Science, Vol. 364, Issue 6438, Page 389-394, Year 2019
掲載日	2019年4月26日
著者	Chao Qi / Simona Sorrentino / Ohad Medalia / Volodymyr M Korkhov /
PubMed 要旨	Membrane-integral adenylyl cyclases (ACs) are key enzymes in mammalian heterotrimeric GTP-binding protein (G protein)-dependent signal transduction, which is important in many cellular processes. ...Membrane-integral adenylyl cyclases (ACs) are key enzymes in mammalian heterotrimeric GTP-binding protein (G protein)-dependent signal transduction, which is important in many cellular processes. Signals received by the G protein-coupled receptors are conveyed to ACs through G proteins to modulate the levels of cellular cyclic adenosine monophosphate (cAMP). Here, we describe the cryo-electron microscopy structure of the bovine membrane AC9 bound to an activated G protein αs subunit at 3.4-angstrom resolution. The structure reveals the organization of the membrane domain and helical domain that spans between the membrane and catalytic domains of AC9. The carboxyl-terminal extension of the catalytic domain occludes both the catalytic and the allosteric sites of AC9, inducing a conformation distinct from the substrate- and activator-bound state, suggesting a regulatory role in cAMP production.
リンク	Science / PubMed:31023924
手法	EM (単粒子)
解像度	3.1 - 4.2 Å
構造データ	4719 6r3q EMDB-4719, PDB-6r3q: The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein 手法: EM (単粒子) / 解像度: 3.4 Å 4721 6r4o EMDB-4721: Structure of a truncated adenylyl cyclase bound to MANT-GTP, forskolin and an activatedstimulatory Galphas protein PDB-6r4o: Structure of a truncated adenylyl cyclase bound to MANT-GTP, forskolin and an activated stimulatory Galphas protein 手法: EM (単粒子) / 解像度: 4.2 Å 4722 6r4p EMDB-4722: Structure of a soluble domain of adenylyl cyclase bound to an activatedstimulatory G protein PDB-6r4p: Structure of a soluble domain of adenylyl cyclase bound to an activated stimulatory G protein 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-4723: Soluble domain of membrane adenylyl cyclase bound to an activated stimulatory G protein, in the presence of MANT-GTP (SOL-M) 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-4724: Structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein (SOL-C map) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-4725: Structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein (AC9-Gas-TM) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-4726: The membrane portion of a membrane adenylyl cyclase bound to an activated stimulatory G protein (TM) 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-GSP: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / GTP-γ-S ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ONM: 3'-O-(N-METHYLANTHRANILOYL)-GUANOSINE-5'-TRIPHOSPHATE / 3′-O-(N-メチルアントラニロイル)GTP ChemComp-FOK: FORSKOLIN / ホルスコリン ChemComp-MN: Unknown entry
由来	bos taurus (ウシ)
キーワード	MEMBRANE PROTEIN / adenylyl cyclase / G protein / occluded state / MANT-GTP / forskolin