EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 1722, Year 2019
掲載日	2019年4月12日
著者	Ricardo D Righetto / Nikhil Biyani / Julia Kowal / Mohamed Chami / Henning Stahlberg /
PubMed 要旨	Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are ...Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are perfectly flat and highly ordered. In practice, such crystals are difficult to obtain. Available image unbending algorithms correct for disorder, but only perform well on images of non-tilted, flat crystals, while out-of-plane distortions are not addressed. Here, we present an approach that employs single-particle refinement procedures to locally unbend crystals in 3D. With this method, density maps of the MloK1 potassium channel with a resolution of 4 Å were obtained from images of 2D crystals that do not diffract beyond 10 Å. Furthermore, 3D classification allowed multiple structures to be resolved, revealing a series of MloK1 conformations within a single 2D crystal. This conformational heterogeneity explains the poor diffraction observed and is related to channel function. The approach is implemented in the FOCUS package.
リンク	Nat Commun / PubMed:30979902 / PubMed Central
手法	EM (単粒子)
解像度	4.0 - 6.0 Å
構造データ	4432 6i9d EMDB-4432: MloK1 consensus map from single particle analysis of 2D crystals PDB-6i9d: MloK1 consensus structure from single particle analysis of 2D crystals 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-4439: Full MloK1 consensus map from single particle analysis of 2D crystals 手法: EM (単粒子) / 解像度: 6.0 Å 4441 6iax EMDB-4441: MloK1 map from single particle analysis of 2D crystals, class 1 (extended conformation) PDB-6iax: MloK1 model from single particle analysis of 2D crystals, class 1 (extended conformation) 手法: EM (単粒子) / 解像度: 5.2 Å 4513 6qcy EMDB-4513: MloK1 map from single particle analysis of 2D crystals, class 2 (intermediate conformation) PDB-6qcy: MloK1 model from single particle analysis of 2D crystals, class 2 (intermediate conformation) 手法: EM (単粒子) / 解像度: 4.7 Å 4514 6qcz EMDB-4514: MloK1 map from single particle analysis of 2D crystals, class 3 (intermediate extended conformation) PDB-6qcz: MloK1 model from single particle analysis of 2D crystals, class 3 (intermediate extended conformation) 手法: EM (単粒子) / 解像度: 4.4 Å 4515 6qd0 EMDB-4515: MloK1 map from single particle analysis of 2D crystals, class 4 (compact/open conformation) PDB-6qd0: MloK1 model from single particle analysis of 2D crystals, class 4 (compact/open conformation) 手法: EM (単粒子) / 解像度: 4.5 Å 4516 6qd1 EMDB-4516: MloK1 map from single particle analysis of 2D crystals, class 5 (intermediate compact conformation) PDB-6qd1: MloK1 model from single particle analysis of 2D crystals, class 5 (intermediate compact conformation) 手法: EM (単粒子) / 解像度: 5.4 Å 4517 6qd2 EMDB-4517: MloK1 map from single particle analysis of 2D crystals, class 6 (intermediate compact conformation) PDB-6qd2: MloK1 model from single particle analysis of 2D crystals, class 6 (intermediate compact conformation) 手法: EM (単粒子) / 解像度: 4.8 Å 4518 6qd3 EMDB-4518: MloK1 map from single particle analysis of 2D crystals, class 7 (intermediate conformation) PDB-6qd3: MloK1 model from single particle analysis of 2D crystals, class 7 (intermediate conformation) 手法: EM (単粒子) / 解像度: 5.0 Å 4519 6qd4 EMDB-4519, PDB-6qd4: MloK1 model from single particle analysis of 2D crystals, class 8 (intermediate conformation) 手法: EM (単粒子) / 解像度: 5.6 Å
化合物	ChemComp-K: Unknown entry / カリウムカチオン
由来	mesorhizobium loti maff303099 (根粒菌) mesorhizobium japonicum (strain lmg 29417 / cect 9101 / maff 303099) (根粒菌)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Voltage-gated potassium channel / Cyclic nucleotide-binding domain / ion channel (イオンチャネル) / ion transport