EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of Respiratory Supercomplex I+III Reveal Functional and Conformational Crosstalk.
ジャーナル・号・ページ	Mol Cell, Vol. 75, Issue 6, Page 1131-11146.e6, Year 2019
掲載日	2019年9月19日
著者	James A Letts / Karol Fiedorczuk / Gianluca Degliesposti / Mark Skehel / Leonid A Sazanov /
PubMed 要旨	The mitochondrial electron transport chain complexes are organized into supercomplexes (SCs) of defined stoichiometry, which have been proposed to regulate electron flux via substrate channeling. We ...The mitochondrial electron transport chain complexes are organized into supercomplexes (SCs) of defined stoichiometry, which have been proposed to regulate electron flux via substrate channeling. We demonstrate that CoQ trapping in the isolated SC I+III limits complex (C)I turnover, arguing against channeling. The SC structure, resolved at up to 3.8 Å in four distinct states, suggests that CoQ oxidation may be rate limiting because of unequal access of CoQ to the active sites of CIII. CI shows a transition between "closed" and "open" conformations, accompanied by the striking rotation of a key transmembrane helix. Furthermore, the state of CI affects the conformational flexibility within CIII, demonstrating crosstalk between the enzymes. CoQ was identified at only three of the four binding sites in CIII, suggesting that interaction with CI disrupts CIII symmetry in a functionally relevant manner. Together, these observations indicate a more nuanced functional role for the SCs.
リンク	Mol Cell / PubMed:31492636 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 7.5 Å
構造データ	4479 6q9b EMDB-4479, PDB-6q9b: CI Membrane Arm focused refinement from Ovine respiratory SC I+III2 手法: EM (単粒子) / 解像度: 3.9 Å 4480 6q9d EMDB-4480, PDB-6q9d: CI Peripheral Arm focused refinement from Ovine respiratory SC I+III2 手法: EM (単粒子) / 解像度: 3.8 Å 4481 6q9e EMDB-4481, PDB-6q9e: Complex III2 focused refinement from Ovine respiratory supercomplex I+III2 手法: EM (単粒子) / 解像度: 3.9 Å 4482 6qa9 EMDB-4482, PDB-6qa9: Isolated complex I class refinement from Ovine respiratory supercomplex I+III2 手法: EM (単粒子) / 解像度: 4.1 Å 4493 6qbx EMDB-4493, PDB-6qbx: Ovine respiratory supercomplex I+III2 closed class. 手法: EM (単粒子) / 解像度: 4.2 Å 4494 6qc2 EMDB-4494, PDB-6qc2: Ovine respiratory supercomplex I+III2 open class 2 手法: EM (単粒子) / 解像度: 4.2 Å 4495 6qc3 EMDB-4495, PDB-6qc3: Ovine respiratory supercomplex I+III2 open class 1 手法: EM (単粒子) / 解像度: 4.2 Å 4496 6qc4 EMDB-4496, PDB-6qc4: Ovine respiratory supercomplex I+III2 open class 3 手法: EM (単粒子) / 解像度: 4.6 Å 4497 6qc5 EMDB-4497, PDB-6qc5: Ovine respiratory complex I FRC closed class 1 手法: EM (単粒子) / 解像度: 4.3 Å 4498 6qc6 EMDB-4498, PDB-6qc6: Ovine respiratory complex I FRC open class 1 手法: EM (単粒子) / 解像度: 4.1 Å 4499 6qc7 EMDB-4499, PDB-6qc7: Ovine respiratory complex I FRC open class 3 手法: EM (単粒子) / 解像度: 4.4 Å 4500 6qc8 EMDB-4500, PDB-6qc8: Ovine respiratory complex I FRC open class 2 手法: EM (単粒子) / 解像度: 4.2 Å 4501 6qc9 EMDB-4501, PDB-6qc9: Ovine respiratory complex I FRC open class 4 手法: EM (単粒子) / 解像度: 5.7 Å 4502 6qca EMDB-4502, PDB-6qca: Ovine respiratory complex I FRC open class 5 手法: EM (単粒子) / 解像度: 6.2 Å 4505 6qcf EMDB-4505, PDB-6qcf: Ovine respiratory complex I FRC open class 6 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-4506: Ovine respiratory complex I FRC poor closed class 手法: EM (単粒子) / 解像度: 7.0 Å EMDB-4507: Ovine respiratory complex I FRC poor open class 手法: EM (単粒子) / 解像度: 7.5 Å
化合物	ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN / フラビンモノヌクレオチド ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-ZN: Unknown entry ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH / ニコチンアミドアデニンジヌクレオチドリン酸 ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE / Heme B ChemComp-U10: UBIQUINONE-10 / 2-(3,7,11,15,19,23,27,31,35,39-デカメチルテトラコンタ-2,6,10,14,18,22,(以下略) / ユビキノン ChemComp-HEC*: HEME C / Heme C
由来	ovis aries (ヒツジ) Sheep (ヒツジ)
キーワード	ELECTRON TRANSPORT (電子伝達系) / complex I (NADH:ユビキノン還元酵素 (水素イオン輸送型)) / membrane arm / cellular respiration (細胞呼吸) / mitochondria (ミトコンドリア) / peripheral arm / complex III (ユビキノール-シトクロムcレダクターゼ) / supercomplex