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-Structure paper
タイトル | How a circularized tmRNA moves through the ribosome. |
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ジャーナル・号・ページ | Science, Vol. 363, Issue 6428, Page 740-744, Year 2019 |
掲載日 | 2019年2月15日 |
著者 | Christopher D Rae / Yuliya Gordiyenko / V Ramakrishnan / |
PubMed 要旨 | During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo- ...During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA. |
リンク | Science / PubMed:30765567 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.7 - 4.3 Å |
構造データ | EMDB-4475, PDB-6q95: EMDB-4476, PDB-6q97: |
化合物 | ChemComp-MG: ChemComp-ZN: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / tmRNA SmpB trans-translation translation |