タイトル | Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility. |
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ジャーナル・号・ページ | Science, Vol. 366, Year 2019 |
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掲載日 | 2018年9月12日 (構造データの登録日) |
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著者 | Reimer, J.M. / Eivaskhani, M. / Harb, I. / Guarne, A. / Weigt, M. / Schmeing, T.M. |
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リンク | Science / PubMed:31699907 |
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手法 | X線回折 |
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解像度 | 2.2 - 6 Å |
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構造データ | PDB-6mfw: Crystal structure of a 4-domain construct of LgrA in the substrate donation state 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-6mfx: Crystal structure of a 4-domain construct of a mutant of LgrA in the substrate donation state 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å PDB-6mfy: Crystal structure of a 5-domain construct of LgrA in the substrate donation state 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-6mfz: Crystal structure of dimodular LgrA in a condensation state 手法: X-RAY DIFFRACTION / 解像度: 6.0 Å PDB-6mg0: Crystal structure of a 5-domain construct of LgrA in the thiolation state 手法: X-RAY DIFFRACTION / 解像度: 6.0 Å |
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化合物 | ChemComp-JQG: (2~{R})-~{N}-[3-[2-[[(2~{S})-2-formamido-3-methyl-butanoyl]amino]ethylamino]-3-oxidanylidene-propyl]-3,3-dimethyl-2-oxidanyl-4-[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxy-butanamide
ChemComp-FON: N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid / (6R)-5-ホルミルテトラヒドロ葉酸
ChemComp-9EF: N-[2-(acetylamino)ethyl]-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide
ChemComp-DG9: 5'-({[(2R,3R)-3-amino-2-{[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-{[oxido(oxo)phosphonio]oxy}butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}-4-methylpentyl]sulfonyl}amino)-5'-deoxyadenosine
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由来 | brevibacillus parabrevis (バクテリア)
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キーワード | LIGASE / nonribosomal peptide synthetase / tailoring domain / NRPS / enzyme / natural product / linear gramicidin |
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