EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of 90 small ribosomal subunit precursors in transition states.
ジャーナル・号・ページ	Science, Vol. 369, Issue 6510, Page 1477-1481, Year 2020
掲載日	2020年9月18日
著者	Yifei Du / Weidong An / Xing Zhu / Qi Sun / Jia Qi / Keqiong Ye /
PubMed 要旨	The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by ...The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.
リンク	Science / PubMed:32943522
手法	EM (単粒子)
解像度	3.2 - 8.6 Å
構造データ	0949 6lqp EMDB-0949, PDB-6lqp: Cryo-EM structure of 90S small subunit preribosomes in transition states (State A) 手法: EM (単粒子) / 解像度: 3.2 Å 0950 6lqq EMDB-0950, PDB-6lqq: Cryo-EM structure of 90S small subunit preribosomes in transition states (State B) 手法: EM (単粒子) / 解像度: 4.1 Å 0951 6lqr EMDB-0951, PDB-6lqr: Cryo-EM structure of 90S small subunit preribosomes in transition states (State C) 手法: EM (単粒子) / 解像度: 8.6 Å 0952 6lqs EMDB-0952, PDB-6lqs: Cryo-EM structure of 90S small subunit preribosomes in transition states (State D) 手法: EM (単粒子) / 解像度: 3.8 Å 0953 6lqt EMDB-0953, PDB-6lqt: Cryo-EM structure of 90S small subunit preribosomes in transition states (State E) 手法: EM (単粒子) / 解像度: 4.9 Å 0954 6lqu EMDB-0954, PDB-6lqu: Cryo-EM structure of 90S small subunit preribosomes in transition states (State A1) 手法: EM (単粒子) / 解像度: 3.7 Å 0955 6lqv EMDB-0955, PDB-6lqv: Cryo-EM structure of 90S small subunit preribosomes in transition states (State C1) 手法: EM (単粒子) / 解像度: 4.8 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	RIBOSOME / ribosome assembly / 90S to pre-40S transition / cryo-EM / Dhr1