EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
ジャーナル・号・ページ	Mol Cell, Vol. 72, Issue 6, Page 999-1012.e6, Year 2018
掲載日	2018年12月20日
著者	Qin Yu / Kun Qu / Yorgo Modis /
PubMed 要旨	Double-stranded RNA (dsRNA) is a potent proinflammatory signature of viral infection. Long cytosolic dsRNA is recognized by MDA5. The cooperative assembly of MDA5 into helical filaments on dsRNA ...Double-stranded RNA (dsRNA) is a potent proinflammatory signature of viral infection. Long cytosolic dsRNA is recognized by MDA5. The cooperative assembly of MDA5 into helical filaments on dsRNA nucleates the assembly of a multiprotein type I interferon signaling platform. Here, we determined cryoelectron microscopy (cryo-EM) structures of MDA5-dsRNA filaments with different helical twists and bound nucleotide analogs at resolutions sufficient to build and refine atomic models. The structures identify the filament-forming interfaces, which encode the dsRNA binding cooperativity and length specificity of MDA5. The predominantly hydrophobic interface contacts confer flexibility, reflected in the variable helical twist within filaments. Mutation of filament-forming residues can result in loss or gain of signaling activity. Each MDA5 molecule spans 14 or 15 RNA base pairs, depending on the twist. Variations in twist also correlate with variations in the occupancy and type of nucleotide in the active site, providing insights on how ATP hydrolysis contributes to MDA5-dsRNA recognition.
リンク	Mol Cell / PubMed:30449722 / PubMed Central
手法	EM (らせん対称)
解像度	3.68 - 4.16 Å
構造データ	0012 6gjz EMDB-0012, PDB-6gjz: CryoEM structure of the MDA5-dsRNA filament in complex with AMPPNP 手法: EM (らせん対称) / 解像度: 4.06 Å 0023 6gkh EMDB-0023, PDB-6gkh: CryoEM structure of the MDA5-dsRNA filament in complex with ADP-AlF4 手法: EM (らせん対称) / 解像度: 4.06 Å 0024 6gkm EMDB-0024, PDB-6gkm: CryoEM structure of the MDA5-dsRNA filament in complex with ATP (10 mM) 手法: EM (らせん対称) / 解像度: 3.87 Å 0143 6h61 EMDB-0143, PDB-6h61: CryoEM structure of the MDA5-dsRNA filament with 89 degree twist and without nucleotide 手法: EM (らせん対称) / 解像度: 4.02 Å 0145 6h66 EMDB-0145, PDB-6h66: CryoEM structure of the MDA5-dsRNA filament with 93 degree twist and without nucleotide 手法: EM (らせん対称) / 解像度: 4.16 Å 4338 6g19 EMDB-4338, PDB-6g19: CryoEM structure of the MDA5-dsRNA filament with 74-degree helical twist 手法: EM (らせん対称) / 解像度: 3.68 Å 4340 6g1s EMDB-4340, PDB-6g1s: CryoEM structure of the MDA5-dsRNA filament with 87-degree helical twist 手法: EM (らせん対称) / 解像度: 3.93 Å 4341 6g1x EMDB-4341, PDB-6g1x: CryoEM structure of the MDA5-dsRNA filament with 91-degree helical twist 手法: EM (らせん対称) / 解像度: 3.93 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ALF: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM / アデノシン三リン酸
由来	mus musculus (ハツカネズミ) Pseudomonas savastanoi pv. phaseolicola (バクテリア) pseudomonas phage phi6 (ファージ)
キーワード	IMMUNE SYSTEM (免疫系) / Protein-RNA complex / helical filament / ATPase (ATPアーゼ) / innate immune receptor