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-Structure paper
タイトル | Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 3, Issue 12, Page 1429-1440, Year 2018 |
掲載日 | 2018年10月22日 |
![]() | Germán G Sgro / Tiago R D Costa / William Cenens / Diorge P Souza / Alexandre Cassago / Luciana Coutinho de Oliveira / Roberto K Salinas / Rodrigo V Portugal / Chuck S Farah / Gabriel Waksman / ![]() ![]() ![]() |
PubMed 要旨 | Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically ...Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically composed of 12 components that form 2 major assemblies: the inner membrane complex embedded in the inner membrane and the core complex embedded in both the inner and outer membranes. Here we present the 3.3 Å-resolution cryo-electron microscopy model of the T4S system core complex from Xanthomonas citri, a phytopathogen that utilizes this system to kill bacterial competitors. An extensive mutational investigation was performed to probe the vast network of protein-protein interactions in this 1.13-MDa assembly. This structure expands our knowledge of the molecular details of T4S system organization, assembly and evolution. |
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手法 | EM (単粒子) |
解像度 | 3.28 Å |
構造データ | |
由来 |
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![]() | MEMBRANE PROTEIN / core complex / bacterial killing / protein transport / bacterial Type IV Secretion System |