+検索条件
-Structure paper
タイトル | Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. |
---|---|
ジャーナル・号・ページ | Neuron, Vol. 97, Issue 6, Page 1261-11267.e4, Year 2018 |
掲載日 | 2018年3月21日 |
![]() | Ying Liu / Tuhin Bhowmick / Yiqiong Liu / Xuefan Gao / Haydyn D T Mertens / Dmitri I Svergun / Junyu Xiao / Yan Zhang / Jia-Huai Wang / Rob Meijers / ![]() ![]() ![]() |
PubMed 要旨 | Axon guidance involves the spatiotemporal interplay between guidance cues and membrane-bound cell-surface receptors, present on the growth cone of the axon. Netrin-1 is a prototypical guidance cue ...Axon guidance involves the spatiotemporal interplay between guidance cues and membrane-bound cell-surface receptors, present on the growth cone of the axon. Netrin-1 is a prototypical guidance cue that binds to deleted in colorectal cancer (DCC), and it has been proposed that the guidance cue Draxin modulates this interaction. Here, we present structural snapshots of Draxin/DCC and Draxin/Netrin-1 complexes, revealing a triangular relationship that affects Netrin-mediated haptotaxis and fasciculation. Draxin interacts with DCC through the N-terminal four immunoglobulin domains, and Netrin-1 through the EGF-3 domain, in the same region where DCC binds. Netrin-1 and DCC bind to adjacent sites on Draxin, which appears to capture Netrin-1 and tether it to the DCC receptor. We propose the conformational flexibility of the single-pass membrane receptor DCC is used to promote fasciculation and regulate axon guidance through concerted Netrin-1/Draxin binding. VIDEO ABSTRACT. |
![]() | ![]() ![]() ![]() |
手法 | SAS (X-ray synchrotron) / X線回折 |
解像度 | 2.493 - 3.07 Å |
構造データ | ![]() SASDBZ6: ![]() PDB-5z5k: ![]() PDB-6fkq: |
化合物 | ![]() ChemComp-NAG: ![]() ChemComp-HOH: ![]() ChemComp-CA: ![]() ChemComp-SO4: ![]() ChemComp-GOL: |
由来 |
|
![]() | APOPTOSIS/INBITITOR / axon guidance / APOPTOSIS-INBITITOR complex / SIGNALING PROTEIN / AXON GUIDANCE CUE / NETRIN-1 / DRAXIN / COMMISSURAL NEURON |