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-Structure paper
タイトル | Shielding and activation of a viral membrane fusion protein. |
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ジャーナル・号・ページ | Nat Commun, Vol. 9, Issue 1, Page 349, Year 2018 |
掲載日 | 2018年1月24日 |
著者 | Steinar Halldorsson / Sai Li / Mengqiu Li / Karl Harlos / Thomas A Bowden / Juha T Huiskonen / |
PubMed 要旨 | Entry of enveloped viruses relies on insertion of hydrophobic residues of the viral fusion protein into the host cell membrane. However, the intermediate conformations during fusion remain unknown. ...Entry of enveloped viruses relies on insertion of hydrophobic residues of the viral fusion protein into the host cell membrane. However, the intermediate conformations during fusion remain unknown. Here, we address the fusion mechanism of Rift Valley fever virus. We determine the crystal structure of the Gn glycoprotein and fit it with the Gc fusion protein into cryo-electron microscopy reconstructions of the virion. Our analysis reveals how the Gn shields the hydrophobic fusion loops of the Gc, preventing premature fusion. Electron cryotomography of virions interacting with membranes under acidic conditions reveals how the fusogenic Gc is activated upon removal of the Gn shield. Repositioning of the Gn allows extension of Gc and insertion of fusion loops in the outer leaflet of the target membrane. These data show early structural transitions that enveloped viruses undergo during host cell entry and indicate that analogous shielding mechanisms are utilized across diverse virus families. |
リンク | Nat Commun / PubMed:29367607 / PubMed Central |
手法 | EM (単粒子) / EM (サブトモグラム平均) / X線回折 |
解像度 | 1.6 - 20.0 Å |
構造データ | EMDB-4197: Icosahedral reconstruction of Rift Valley fever virus virion EMDB-4198: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 1 EMDB-4199: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 2 EMDB-4200: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 3 EMDB-4201: Localized reconstruction of the Rift Valley fever virus glycoprotein pentamer EMDB-4202: EMDB-4203: EMDB-4204: EMDB-4205: EMDB-4206: EMDB-4207: EMDB-4208: EMDB-4209: EMDB-4210: EMDB-4211: PDB-6f8p: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | VIRAL PROTEIN / phlebovirus / glycoprotein / bunyavirus / host cell entry / VIRUS / enveloped virus / RVFV / fusion protein |