EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	HIV-1 Nefs Are Cargo-Sensitive AP-1 Trimerization Switches in Tetherin Downregulation.
ジャーナル・号・ページ	Cell, Vol. 174, Issue 3, Page 659-671.e14, Year 2018
掲載日	2018年7月26日
著者	Kyle L Morris / Cosmo Z Buffalo / Christina M Stürzel / Elena Heusinger / Frank Kirchhoff / Xuefeng Ren / James H Hurley /
PubMed 要旨	The HIV accessory protein Nef counteracts immune defenses by subverting coated vesicle pathways. The 3.7 Å cryo-EM structure of a closed trimer of the clathrin adaptor AP-1, the small GTPase Arf1, ...The HIV accessory protein Nef counteracts immune defenses by subverting coated vesicle pathways. The 3.7 Å cryo-EM structure of a closed trimer of the clathrin adaptor AP-1, the small GTPase Arf1, HIV-1 Nef, and the cytosolic tail of the restriction factor tetherin suggested a mechanism for inactivating tetherin by Golgi retention. The 4.3 Å structure of a mutant Nef-induced dimer of AP-1 showed how the closed trimer is regulated by the dileucine loop of Nef. HDX-MS and mutational analysis were used to show how cargo dynamics leads to alternative Arf1 trimerization, directing Nef targets to be either retained at the trans-Golgi or sorted to lysosomes. Phosphorylation of the NL4-3 M-Nef was shown to regulate AP-1 trimerization, explaining how O-Nefs lacking this phosphosite counteract tetherin but most M-Nefs do not. These observations show how the higher-order organization of a vesicular coat can be allosterically modulated to direct cargoes to distinct fates.
リンク	Cell / PubMed:30053425 / PubMed Central
手法	EM (単粒子)
解像度	3.73 - 6.8 Å
構造データ	7453 6d83 EMDB-7453: Structure of the HIV-Nef dileucine mutant bound AP-1:Arf1 dimer monomeric subunit PDB-6d83: Structure of the cargo bound AP-1:Arf1:tetherin-Nef (L164A, L165A) dileucine mutant dimer monomeric subunit 手法: EM (単粒子) / 解像度: 4.27 Å 7454 6d84 EMDB-7454: Structure of the HIV-Nef dileucine mutant bound AP-1:Arf1 dimer PDB-6d84: Structure of the cargo bound AP-1:Arf1:tetherin-Nef (L164A, L165A) dileucine mutant dimer 手法: EM (単粒子) / 解像度: 6.72 Å 7455 6dff EMDB-7455, PDB-6dff: Structure of the cargo bound AP-1:Arf1:tetherin-Nef monomer 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-7456: Structure of the cargo bound AP-1:Arf1:tetherin-Nef closed trimer monomeric subunit after focussed classification 手法: EM (単粒子) / 解像度: 4.05 Å 7457 6cm9 EMDB-7457: Structure of the HIV-Nef bound AP-1:Arf1 closed trimer monomeric subunit PDB-6cm9: Structure of the cargo bound AP-1:Arf1:tetherin-Nef closed trimer monomeric subunit 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-7458: Structure of the cargo bound AP-1:Arf1:tetherin-Nef closed trimer 手法: EM (単粒子) / 解像度: 4.27 Å 7563 6cri EMDB-7563: Structure of the HIV-Nef tetherin cargo bound AP-1:Arf1 stable closed trimer PDB-6cri: Structure of the cargo bound AP-1:Arf1:tetherin-Nef stable closed trimer 手法: EM (単粒子) / 解像度: 6.8 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト) human immunodeficiency virus 1 (ヒト免疫不全ウイルス) mus musculus (ハツカネズミ)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / PROTEIN TRANSPORT / AP / HIV (ヒト免疫不全ウイルス) / Nef / trafficking / TRANSPORT PROTEIN (運搬体タンパク質)