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-Structure paper
タイトル | Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate. |
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ジャーナル・号・ページ | Protein Cell, Vol. 10, Issue 2, Page 120-130, Year 2019 |
掲載日 | 2018年3月19日 |
著者 | Dejian Zhou / Xing Zhu / Sanduo Zheng / Dan Tan / Meng-Qiu Dong / Keqiong Ye / |
PubMed 要旨 | Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is ...Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits. |
リンク | Protein Cell / PubMed:29557065 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.294 - 3.65 Å |
構造データ | EMDB-6878, PDB-5z3g: PDB-5z1g: |
化合物 | ChemComp-SO4: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / BRIX domain / pre-60S / pre-ribosome / protein-RNA complex |