EMDB-6878: Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)

Basic information

• Entry: Database: EMDB / ID: EMD-6878
• Title: Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)
• Map data: Unmasked cryo-EM map

Sample

• Complex: Rpf1-TAP pre-60S
  • RNA: x 3 types
  • Protein or peptide: x 32 types

• Keywords: ribosome / pre-60S / pre-ribosome / protein-RNA complex

Function / homology

Function:

snoRNA release from pre-rRNA / nuclear division / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / PeBoW complex / ATP-dependent activity, acting on RNA / rRNA primary transcript binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of 5.8S rRNA / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / proteasome binding / Major pathway of rRNA processing in the nucleolus and cytosol / ribosomal large subunit binding / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / preribosome, large subunit precursor / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / ribonucleoprotein complex binding / ribosomal subunit export from nucleus / maturation of LSU-rRNA / translation initiation factor activity / nuclear periphery / proteasome complex / assembly of large subunit precursor of preribosome / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / macroautophagy / small-subunit processome / protein catabolic process / maintenance of translational fidelity / rRNA processing / nuclear envelope / ribosomal small subunit biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / protein-macromolecule adaptor activity / cytosolic large ribosomal subunit / cytoplasmic translation / RNA helicase activity / negative regulation of translation / rRNA binding / structural constituent of ribosome / RNA helicase / ribosome / translation / mRNA binding / nucleolus / ATP hydrolysis activity / RNA binding / zinc ion binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytoplasm / cytosol

Domain/homology:

WDR74/Nsa1 / Ribosomal RNA processing protein 1-like / Nucleolar protein,Nop52 / Mak16 protein / Mak16 protein C-terminal region / Ribosome biogenesis protein 15, RNA recognition motif / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / Ribosome biogenesis protein BRX1 / DDX18/Has1, DEAD-box helicase domain / Domain of unknown function DUF4217 / ATP-dependent rRNA helicase SPB4-like, C-terminal extension / DUF4217 / U3 snoRNP protein/Ribosome production factor 1 / Pescadillo / Pescadillo N-terminus / BRCT domain / Brix domain / Brix domain / Brix domain profile. / Brix / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / DEAD-box RNA helicase Q motif profile. / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / breast cancer carboxy-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / : / Ribosomal protein L6e signature. / Ribosomal protein L13e / Ribosomal protein L13e / BRCT domain profile. / 60S ribosomal protein L18a/ L20, eukaryotes / BRCT domain / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L36e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / BRCT domain superfamily / Ribosomal Protein L6, KOW domain / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / 60S ribosomal protein L35 / Ribosomal protein L6e / 60S ribosomal protein L6E / Ribosomal protein L7A/L8 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L18e / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L14e domain / Ribosomal protein L14 / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L6, conserved site-2 / Ribosomal protein L6 signature 2. / Ribosomal protein L14 / Ribosomal protein L15e, conserved site / Ribosomal protein L15e signature. / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal_L15e / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal L15 / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L32e / Ribosomal protein L37ae/L37e / Ribosomal protein L32e superfamily / Ribosomal protein L32 / Ribosomal_L32e / Ribosomal protein L26/L24, eukaryotic/archaeal

Component:

Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein uL4A / Protein MAK16 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL13A / Ribosomal RNA-processing protein 1 / rRNA-processing protein EBP2 / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Proteasome-interacting protein CIC1 / Ribosome production factor 1 / Ribosome biogenesis protein RLP7 / Large ribosomal subunit protein eL37A / Ribosome biogenesis protein NSA1 / Pescadillo homolog / Ribosome biogenesis protein 15 / Large ribosomal subunit protein eL6A / ATP-dependent RNA helicase HAS1 / Ribosome biogenesis protein BRX1 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein eL13A

• Biological species: Saccharomyces cerevisiae (brewer's yeast) / Saccharomyces cerevisiae S288c (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 3.65 Å
• Authors: Zhou D / Zhu X / Ye K
• Citation: Journal: Protein Cell / Year: 2019; Title: Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.; Authors: Dejian Zhou / Xing Zhu / Sanduo Zheng / Dan Tan / Meng-Qiu Dong / Keqiong Ye / ; Abstract: Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.

History

Deposition	Jan 6, 2018	-
Header (metadata) release	Apr 11, 2018	-
Map release	Apr 11, 2018	-
Update	Nov 6, 2024	-
Current status	Nov 6, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_6878.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Unmasked cryo-EM map
• Voxel size: X=Y=Z: 1.38 Å

Density

Contour Level	By AUTHOR: 0.09 / Movie #1: 0.09
Minimum - Maximum	-0.2651229 - 0.5640423
Average (Standard dev.)	0.0001233261 (±0.018730178)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 496.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.38	1.38	1.38
M x/y/z	360	360	360
origin x/y/z	0.000	0.000	0.000
length x/y/z	496.800	496.800	496.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	360	360	360
D min/max/mean	-0.265	0.564	0.000

Supplemental data

Sample components

Entire : Rpf1-TAP pre-60S

• Entire: Name: Rpf1-TAP pre-60S

Components

• Complex: Rpf1-TAP pre-60S
  • RNA: 25S rRNA
  • RNA: 5.8S rRNA
  • RNA: ITS2 RNA
  • Protein or peptide: Ribosome biogenesis protein RLP7
  • Protein or peptide: Ribosome biogenesis protein 15
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L4-A
  • Protein or peptide: Proteasome-interacting protein CIC1
  • Protein or peptide: 60S ribosomal protein L6-A
  • Protein or peptide: 60S ribosomal protein L7-A
  • Protein or peptide: 60S ribosomal protein L8-A
  • Protein or peptide: 60S ribosomal protein L9-A
  • Protein or peptide: Nucleolar protein 7
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • Protein or peptide: Ribosome production factor 1
  • Protein or peptide: 60S ribosomal protein L13-A
  • Protein or peptide: 60S ribosomal protein L14-A
  • Protein or peptide: 60S ribosomal protein L15-A
  • Protein or peptide: 60S ribosomal protein L16-A
  • Protein or peptide: 60S ribosomal protein L17-A
  • Protein or peptide: 60S ribosomal protein L18-A
  • Protein or peptide: Ribosome biogenesis protein NSA1
  • Protein or peptide: 60S ribosomal protein L20-A
  • Protein or peptide: Ribosomal RNA-processing protein 1
  • Protein or peptide: ATP-dependent RNA helicase HAS1
  • Protein or peptide: Protein MAK16
  • Protein or peptide: Ribosome biogenesis protein BRX1
  • Protein or peptide: rRNA-processing protein EBP2
  • Protein or peptide: 60S ribosomal protein L26-A
  • Protein or peptide: Unassigned
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L33-A
  • Protein or peptide: 60S ribosomal protein L35-A
  • Protein or peptide: 60S ribosomal protein L36-A
  • Protein or peptide: 60S ribosomal protein L37-A

Supramolecule #1: Rpf1-TAP pre-60S

• Supramolecule: Name: Rpf1-TAP pre-60S / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: 25S rRNA

• Macromolecule: Name: 25S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 1.0974938749999998 MDa

Sequence

String:

GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG GCCAGCAUCA GUUUUGGUGG CAGGAUAAAU CCAUAGGAAU GUAGCUUGCC UCGGUAAGUA UUAUAG CCU GUGGGAAUAC UGCCAGCUGG GACUGAGGAC UGCGACGUAA GUCAAGGAUG CUGGCAUAAU GGUUAUAUGC CGCCCGU CU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUA G GUUGGGGCCU CGCAAGAGGU GCACAAUCGA CCGAUCCUGA UGUCUUCGGA UGGAUUUGAG UAAGAGCAUA GCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAUA GGGUGAAGCC AGAGGAAACU CUGGUGGAGG CUCGUAGCGG UUCUGACGUG CAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCUGCC GAAGUUUCCC U CAGGAUAG CAGAAGCUCG UAUCAGUUUU AUGAGGUAAA GCGAAUGAUU AGAGGUUCCG GGGUCGAAAU GACCUUGACC UA UUCUCAA ACUUUAAAUA UGUAAGAAGU CCUUGUUACU UAAUUGAACG UGGACAUUUG AAUGAAGAGC UUUUAGUGGG CCA UUUUUG GUAAGCAGAA CUGGCGAUGC GGGAUGAACC GAACGUAGAG UUAAGGUGCC GGAAUACACG CUCAUCAGAC ACCA CAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACC GGCC GAAUGAACUA GCCCUGAAAA UGGAUGGCGC UCAAGCGUGU UACCUAUACU CUACCGUCAG GGUUGAUAUG AUGCCC UGA CGAGUAGGCA GGCGUGGAGG UCAGUGACGA AGCCUAGACC GUAAGGUCGG GUCGAACGGC CUCUAGUGCA GAUCUUG GU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGG G UUAGUCGAUC CUAAGAGAUG GGGAAGCUCC GUUUCAAAGG CCUGAUUUUA UGCAGGCCAC CAUCGAAAGG GAAUCCGGU UAAGAUUCCG GAACCUGGAU AUGGAUUCUU CACGGUAACG UAACUGAAUG UGGAGACGUC GGCGCGAGCC CUGGGAGGAG UUAUCUUUU CUUCUUAACA GCUUAUCACC CCGGAAUUGG UUUAUCCGGA GAUGGGGUCU UAUGGCUGGA AGAGGCCAGC A CCUUUGCU GGCUCCGGUG CGCUUGUGAC GGCCCGUGAA AAUCCACAGG AAGGAAUAGU UUUCAUGCCA GGUCGUACUG AU AACCGCA GCAGGUCUCC AAGGUGAACA GCCUCUAGUU GAUAGAAUAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGU AACUUC GGGAUAAGGA UUGGCUCUAA GGGUCGGGUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUAAC GAUCAACUUA GAACUGGUAC GGACAAGGGG AAUCUGACUG UCUAAUUAAA ACAUAGCAUU GCGAUG GUC AGAAAGUGAU GUUGACGCAA UGUGAUUUCU GCCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAACCAA GCGCGGG UA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUA A CGAGAUUCCC ACUGUCCCUA UCUACUAUCU AGCGAAACCA CAGCCAAGGG AACGGGCUUG GCAGAAUCAG CGGGGAAAG AAGACCCUGU UGAGCUUGAC UCUAGUUUGA CAUUGUGAAG AGACAUAGAG GGUGUAGAAU AAGUGGGAGC UUCGGCGCCA GUGAAAUAC CACUACCUUU AUAGUUUCUU UACUUAUUCA AUGAAGCGGA GCUGGAAUUC AUUUUCCACG UUCUAGCAUU C AAGGUCCC AUUCGGGGCU GAUCCGGGUU GAAGACAUUG UCAGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC GA UAACGCA GAUGUCCUAA GGGGGGCUCA UGGAGAACAG AAAUCUCCAG UAGAACAAAA GGGUAAAAGC CCCCUUGAUU UUG AUUUUC AGUGUGAAUA CAAACCAUGA AAGUGUGGCC UAUCGAUCCU UUAGUCCCUC GGAAUUUGAG GCUAGAGGUG CCAG AAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCU CUUC CUAUCAUACC GAAGCAGAAU UCGGUAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACC GUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AAUGUUACCG CAAUAGUAAU UGAACUUAGU ACGAGAGGAA CAGUUCA UU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUC U AAGUCAGAAU CCAUGCUAGA ACGCGGUGAU UUCUUUGCUC CACACAAUAU AGAUGGAUAC GAAUAAGGCG UCCUUGUGG CGUCGCUGAA CCAUAGCAGG CUAGCAACGG UGCACUUGGC GGAAAGGCCU UGGGUGCUUG CUGGCGAAUU GCAAUGUCAU UUUGCGUGG GGAUAAAUCA UUUGUAUACG ACUUAGAUGU ACAACGGGGU AUUGUAAGCA GUAGAGUAGC CUUGUUGUUA C GAUCUGCU GAGAUUAAGC CUUUGUUGUC UGAUUUGU

GENBANK: GENBANK: CP011821.1

Macromolecule #2: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MG214540.1

Macromolecule #3: ITS2 RNA

• Macromolecule: Name: ITS2 RNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 74.308391 KDa

Sequence

String:

CCUUCUCAAA CAUUCUGUUU GGUAGUGAGU GAUACUCUUU GGAGUUAACU UGAAAUUGCU GGCCUUUUCA UUGGAUGUUU UUUUUCCAA AGAGAGGUUU CUCUGCGUGC UUGAGGUAUA AUGCAAGUAC GGUCGUUUUA GGUUUUACCA ACUGCGGCUA A UCUUUUUU UAUACUGAGC GUAUUGGAAC GUUAUCGAUA AGAAGAGAGC GUCUAGGCGA ACAAUGUUCU UAAA

GENBANK: GENBANK: KY203910.1

Macromolecule #4: Ribosome biogenesis protein RLP7

• Macromolecule: Name: Ribosome biogenesis protein RLP7 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 36.621074 KDa

Sequence

String:

MSSTQDSKAQ TLNSNPEILL RKRRNADRTR IERQELAKKK REEQIKKKRS NKNKFVRAES IVAKTLATSR EKERIKRVSI LEDKKAKNE TQHIASGKDF ILKITEKANG AEENSVDLEE TEEEEDDGLI REKTTYDGKP ALLFIVRVRG PLAVNIPNKA F KILSLLRL VETNTGVFVK LTKNVYPLLK VIAPYVVIGK PSLSSIRSLI QKRGRIIYKG ENEAEPHEIV LNDNNIVEEQ LG DHGIICV EDIIHEIATM GESFSVCNFF LQPFKLNREV SGFGSLNRLR KIKQREAESR TRQFSNAATA PVIEVDIDSL LAK LN

UniProtKB: Ribosome biogenesis protein RLP7

Macromolecule #5: Ribosome biogenesis protein 15

• Macromolecule: Name: Ribosome biogenesis protein 15 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.499186 KDa

Sequence

String:

MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEY SGIIYVSRLP HGFHEKELSK YFAQFGDLKE VRLARNKKTG NSRHYGFLEF VNKEDAMIAQ ESMNNYLLMG H LLQVRVLP KGAKIEKLYK YKKRVLVEKG ITKPVKQLKD NMKQKHEERI KKLAKSGIEF KW

UniProtKB: Ribosome biogenesis protein 15

Macromolecule #6: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 43.850793 KDa

Sequence

String:

MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #7: 60S ribosomal protein L4-A

• Macromolecule: Name: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 39.159125 KDa

Sequence

String:

MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

UniProtKB: Large ribosomal subunit protein uL4A

Macromolecule #8: Proteasome-interacting protein CIC1

• Macromolecule: Name: Proteasome-interacting protein CIC1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 42.596691 KDa

Sequence

String:

MAKKSNSKKS TPVSTPSKEK KKVIEKKSST AIPRERVIKA VNELIKFTSK PQDENNEEGN NGKKNLLEDD EEELKKDLQL IVVNNKSFT GTSKSFKLKL LNVKHSFYKP WKEASATAVK DFKVLLILKD SDIKKVSEDD LFDQLDSEGI KVDEIICGKD L KTVYKAYE ARNAFISQFS LILADDSIVT SLPKLMGGKA YNKVETTPIS IRTHANKEFS LTTLTNNIKK VYMNQLPVKL PR GTTLNVH LGNLEWLRPE EFVDNVELIS EQLIKAYQIR SIFIKTNRSP VLPLYYNQDV LDELEAKKDK IEETHEDDMV TID GVQVHL STFNKGLMEI ANPSELGSIF SKQINNAKKR SSSELEKESS ESEAVKKAKS

UniProtKB: Proteasome-interacting protein CIC1

Macromolecule #9: 60S ribosomal protein L6-A

• Macromolecule: Name: 60S ribosomal protein L6-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.000564 KDa

Sequence

String:

MSAQKAPKWY PSEDVAALKK TRKAARPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLISGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQNKE IKAERVEDQK VVDKALIAEI KKTPLLKQYL S ASFSLKNG DKPHMLKF

UniProtKB: Large ribosomal subunit protein eL6A

Macromolecule #10: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.686281 KDa

Sequence

String:

MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #11: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 28.17582 KDa

Sequence

String:

MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #12: 60S ribosomal protein L9-A

• Macromolecule: Name: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

Macromolecule #13: Nucleolar protein 7

• Macromolecule: Name: Nucleolar protein 7 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 69.984148 KDa

Sequence

String:

MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARK LTRALGRGEV SSAKRLEENR DSYTLDHIIK ERYPSFPDAI RDIDDALNML FLFSNLPSTN QVSSKIINDA Q KICNQWLA YVAKERLVRK VFVSIKGVYY QANIKGEEVR WLVPFKFPEN IPSDVDFRIM LTFLEFYSTL LHFVLYKLYT DS GLIYPPK LDLKKDKIIS GLSSYILESR QEDSLLKLDP TEIEEDVKVE SLDASTLKSA LNADEANTDE TEKEEEQEKK QEK EQEKEQ NEETELDTFE DNNKNKGDIL IQPSKYDSPV ASLFSAFVFY VSREVPIDIL EFLILSCGGN VISEAAMDQI ENKK DIDMS KVTHQIVDRP VLKNKVAGRT YIQPQWIFDC INKGELVPAN KYLPGEALPP HLSPWGDAIG YDPTAPVEEG EEEES ESES ESEDQVEEED QEVVAGEEDD DDDEELQAQK ELELEAQGIK YSETSEADKD VNKSKNKKRK VDEEEEEKKL KMIMMS NKQ KKLYKKMKYS NAKKEEQAEN LKKKKKQIAK QKAKLNKLDS KK

UniProtKB: Pescadillo homolog

Macromolecule #14: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 26.476605 KDa

Sequence

String:

MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ DQEELSSLLQ VPLVAGTVNR GSSVVGAGMV VNDYLAVTGL DTTAPELSVI ESIFRLQDAQ PESISGNLRD TL IETYS

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #15: Ribosome production factor 1

• Macromolecule: Name: Ribosome production factor 1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 35.184387 KDa

Sequence

String:

MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ RLKENVTQTI ENTRVYDETI NKEVEGDEDD LMRYFNSNS NEPPKIFLTT NVNAKKSAYE FANILIEILP NVTFVKRKFG YKLKEISDIC IKRNFTDIVI INEDKKKVTG L TFIHLPEG PTFYFKLSSF VEVKKIVGHG RPTSHIPELI LNNFQTRLGQ TVGRLFQSIL PQNPDIEGRQ VITLHNQRDY IF FRRHRYV FKDNERVGLQ ELGPQFTLKL KRLQRGIKEE TEWEHKPEMD KEKKKFYL

UniProtKB: Ribosome production factor 1

Macromolecule #16: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.604164 KDa

Sequence

String:

MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

UniProtKB: Large ribosomal subunit protein eL13A

Macromolecule #17: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.195066 KDa

Sequence

String:

MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #18: 60S ribosomal protein L15-A

• Macromolecule: Name: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.482357 KDa

Sequence

String:

MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

UniProtKB: Large ribosomal subunit protein eL15A

Macromolecule #19: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.247227 KDa

Sequence

String:

MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #20: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.589518 KDa

Sequence

String:

MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #21: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.609252 KDa

Sequence

String:

MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

Macromolecule #22: Ribosome biogenesis protein NSA1

• Macromolecule: Name: Ribosome biogenesis protein NSA1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 51.982457 KDa

Sequence

String:

MRLLVSCVDS GSIKEVLCNI GTDTSVQSAL QPFHVAPHLA EGLKAYVDRM WVISEDEAIL ARNSGVVELV KISKHLKENE ALQVDPKGE SKNEKSLSDD LPKFDISEFE ITSSVSDLFD DAKLESLSSK SVKRTKLVDG FVTLCPIKKD SSNNTFVAAT K SGLLHIIK KGEDKKLIKL ASLGLKAPVE FLQLYDLEDT DTDKYIFAYG GEENLIKLVE IDSSFQSLKQ IWEAKNVKND RL DMRVPVW PMALRFLEPS PGKTEKGKLN YQFAAITRWS HLTKYSTQHG RKPFAQIDLL PNREPLSQME VFDAKGENVV SSL GNFQSE TFNELNVITT DYKKNVFKFD GNGRMLGKVG RDDITGSSTY IHVHDGKYLL QGGLDRYVRI FDIKTNKMLV KVYV GSRIN FIVMLDDVEI EMPLSPSAKA AKGKQKRKVT ELEEDADELW NKLEGKVAAS KASKKSKI

UniProtKB: Ribosome biogenesis protein NSA1

Macromolecule #23: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.478852 KDa

Sequence

String:

MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #24: Ribosomal RNA-processing protein 1

• Macromolecule: Name: Ribosomal RNA-processing protein 1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.250531 KDa

Sequence

String:

METSNFVKQL SSNNRKTRVN ALEALKKYLT AKQFKENKQI EFNKLWKGLY YAMWFSDRPR PQQRLANELG ELHGLYFDPK DNSTADELT TNDKAFIKFS RGFWKVMCFE WFNIDRYRLD KYLLLIRRVL FSQLKYLQSR NWDKKLVDEY IKKVLRWLPL S GSPKVYTG IPIHIVDILL DEWERLLKDG DEDDEDEENK EEEMRKIAES AKKTPLADVI AIFQDIVADY NNSKVLREKI KE DLFSDTR LVSWDILEGE TQHNDSSNES EEEEEEEWKG F

UniProtKB: Ribosomal RNA-processing protein 1

Macromolecule #25: ATP-dependent RNA helicase HAS1

• Macromolecule: Name: ATP-dependent RNA helicase HAS1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 56.798348 KDa

Sequence

String:

MATPSNKRSR DSESTEEPVV DEKSTSKQNN AAPEGEQTTC VEKFEELKLS QPTLKAIEKM GFTTMTSVQA RTIPPLLAGR DVLGAAKTG SGKTLAFLIP AIELLHSLKF KPRNGTGIIV ITPTRELALQ IFGVARELME FHSQTFGIVI GGANRRQEAE K LMKGVNML IATPGRLLDH LQNTKGFVFK NLKALIIDEA DRILEIGFED EMRQIIKILP NEDRQSMLFS ATQTTKVEDL AR ISLRPGP LFINVVPETD NSTADGLEQG YVVCDSDKRF LLLFSFLKRN QKKKIIVFLS SCNSVKYYAE LLNYIDLPVL ELH GKQKQQ KRTNTFFEFC NAERGILICT DVAARGLDIP AVDWIIQFDP PDDPRDYIHR VGRTARGTKG KGKSLMFLTP NELG FLRYL KASKVPLNEY EFPENKIANV QSQLEKLIKS NYYLHQTAKD GYRSYLQAYA SHSLKTVYQI DKLDLAKVAK SYGFP VPPK VNITIGASGK TPNTKRRKTH K

UniProtKB: ATP-dependent RNA helicase HAS1

Macromolecule #26: Protein MAK16

• Macromolecule: Name: Protein MAK16 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 35.754605 KDa

Sequence

String:

MSDEIVWQVI NQSFCSHRIK APNGQNFCRN EYNVTGLCTR QSCPLANSKY ATVKCDNGKL YLYMKTPERA HTPAKLWERI KLSKNYTKA LQQIDEHLLH WSKFFRHKCK QRFTKLTQVM ITERRLALRE EERHYVGVAP KVKRREQNRE RKALVAAKIE K AIEKELMD RLKSGAYGDK PLNVDEKVWK KIMGQMEEEN SQDEEEDWDE EEESDDGEVE YVADDGEGEY VDVDDLEKWL AD SDREASS ASESESDSES ESDSDSDEEN KNSAKRRKKG TSAKTKRPKV EIEYEEEHEV QNAEQEVAQ

UniProtKB: Protein MAK16

Macromolecule #27: Ribosome biogenesis protein BRX1

• Macromolecule: Name: Ribosome biogenesis protein BRX1 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.585414 KDa

Sequence

String:

MSSIYKALAG KSKDNKSEKK QGNVKQFMNK QRTLLISSRG VNYRHRHLIQ DLSGLLPHSR KEPKLDTKKD LQQLNEIAEL YNCNNVLFF EARKHQDLYL WLSKPPNGPT IKFYIQNLHT MDELNFTGNC LKGSRPVLSF DQRFESSPHY QLIKELLVHN F GVPPNARK SKPFIDHVMS FSIVDDKIWV RTYEISHSTK NKEEYEDGEE DISLVEIGPR FVMTVILILE GSFGGPKIYE NK QYVSPNV VRAQIKQQAA EEAKSRAEAA VERKIKRREN VLAADPLSND ALFK

UniProtKB: Ribosome biogenesis protein BRX1

Macromolecule #28: rRNA-processing protein EBP2

• Macromolecule: Name: rRNA-processing protein EBP2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 49.842613 KDa

Sequence

String:

MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK LEKKEKKADV KKEVAADTEE YQSQALSKKE KRKLKKELK KMQEQDATEA QKHMSGDEDE SGDDREEEEE EEEEEEGRLD LEKLAKSDSE SEDDSESEND SEEDEDVVAK E ESEEKEEQ EEEQDVPLSD VEFDSDADVV PHHKLTVNNT KAMKHALERV QLPWKKHSFQ EHQSVTSETN TDEHIKDIYD DT ERELAFY KQSLDAVLVA RDELKRLKVP FKRPLDYFAE MVKSDEHMDK IKGKLIEEAS DKKAREEARR QRQLKKFGKQ VQN ATLQKR QLEKRETLEK IKSLKNKRKH NEIDHSEFNV GVEEEVEGKR FDRGRPNGKR AAKNAKYGQG GMKRFKRKND ATSS ADVSG FSSRKMKGKT NRPGKSRRAR RF

UniProtKB: rRNA-processing protein EBP2

Macromolecule #29: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.265784 KDa

Sequence

String:

MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #30: Unassigned

• Macromolecule: Name: Unassigned / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.12417 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #31: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.809441 KDa

Sequence

String:

MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #32: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.17713 KDa

Sequence

String:

MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #33: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.94264 KDa

Sequence

String:

MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #34: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.151259 KDa

Sequence

String:

MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #35: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.877395 KDa

Sequence

String:

MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

UniProtKB: Large ribosomal subunit protein eL37A

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

Buffer

pH: 8
Component:

Concentration	Name	Formula
10.0 mM	Tris-HCl
100.0 mM	sodium chloride	NaCl
2.0 mM	DTT

• Grid: Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: blot for 1-3 seconds before plunging.

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Number grids imaged: 1 / Number real images: 1983 / Average exposure time: 1.5 sec. / Average electron dose: 30.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 331816

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

4v88

Details: 40S ribosome

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.65 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 98155
• Initial angle assignment: Type: NOT APPLICABLE / Software - Name: RELION (ver. 1.4)
• Final angle assignment: Type: NOT APPLICABLE / Software - Name: RELION (ver. 1.4)
• Final 3D classification: Number classes: 6 / Software - Name: RELION (ver. 1.4)

Atomic model buiding 1

• Details: real space refinement
• Refinement: Space: REAL

Output model

PDB-5z3g:
Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)