EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The atomic structures of shrimp nodaviruses reveal new dimeric spike structures and particle polymorphism.
ジャーナル・号・ページ	Commun Biol, Vol. 2, Page 72, Year 2019
掲載日	2019年2月20日
著者	Nai-Chi Chen / Masato Yoshimura / Naoyuki Miyazaki / Hong-Hsiang Guan / Phimonphan Chuankhayan / Chien-Chih Lin / Shao-Kang Chen / Pei-Ju Lin / Yen-Chieh Huang / Kenji Iwasaki / Atsushi Nakagawa / Sunney I Chan / Chun-Jung Chen /
PubMed 要旨	Shrimp nodaviruses, including (PvNV) and nodaviruses (MrNV), cause white-tail disease in shrimps, with high mortality. The viral capsid structure determines viral assembly and host specificity ...Shrimp nodaviruses, including (PvNV) and nodaviruses (MrNV), cause white-tail disease in shrimps, with high mortality. The viral capsid structure determines viral assembly and host specificity during infections. Here, we show cryo-EM structures of = 3 and = 1 PvNV-like particles (PvNV-LPs), crystal structures of the protrusion-domains (P-domains) of PvNV and MrNV, and the crystal structure of the ∆N-ARM-PvNV shell-domain (S-domain) in = 1 subviral particles. The capsid protein of PvNV reveals five domains: the P-domain with a new jelly-roll structure forming cuboid-like spikes; the jelly-roll S-domain with two calcium ions; the linker between the S- and P-domains exhibiting new cross and parallel conformations; the N-arm interacting with nucleotides organized along icosahedral two-fold axes; and a disordered region comprising the basic -terminal arginine-rich motif (N-ARM) interacting with RNA. The N-ARM controls = 3 and = 1 assemblies. Increasing the /-termini flexibility leads to particle polymorphism. Linker flexibility may influence the dimeric-spike arrangement.
リンク	Commun Biol / PubMed:30820467 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.17 - 3.7 Å
構造データ	6999 6ab5 EMDB-6999, PDB-6ab5: Cryo-EM structure of T=1 Penaeus vannamei nodavirus 手法: EM (単粒子) / 解像度: 3.7 Å 9576 6ab6 EMDB-9576, PDB-6ab6: Cryo-EM structure of T=3 Penaeus vannamei nodavirus 手法: EM (単粒子) / 解像度: 3.5 Å PDB-5yku: The crystal structure of Macrobrachium rosenbergii nodavirus P-domain with Zn ions 手法: X-RAY DIFFRACTION / 解像度: 1.39 Å PDB-5ykv: The crystal structure of Macrobrachium rosenbergii nodavirus P-domain 手法: X-RAY DIFFRACTION / 解像度: 2.31 Å PDB-5ykx: The crystal structure of Macrobrachium rosenbergii nodavirus P-domain with Cd ion 手法: X-RAY DIFFRACTION / 解像度: 2.0 Å PDB-5ykz: The crystal structure of Penaeus vannamei nodavirus P-domain (P21) 手法: X-RAY DIFFRACTION / 解像度: 1.17 Å PDB-5yl0: The crystal structure of Penaeus vannamei nodavirus P-domain (P212121) 手法: X-RAY DIFFRACTION / 解像度: 1.22 Å PDB-5yl1: T=1 subviral particle of Penaeus vannamei nodavirus capsid protein deletion mutant (delta 1-37 & 251-368) 手法: X-RAY DIFFRACTION / 解像度: 3.12 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-HOH: WATER / 水 ChemComp-CD: Unknown entry ChemComp-EPE: 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES / pH緩衝剤YM / HEPES ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-CA*: Unknown entry / カルシウムジカチオン
由来	penaeus vannamei nodavirus (ウイルス) macrobrachium rosenbergii nodavirus (ウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / Protrusion domain of viral capsid protein / viral capsid protein (カプシド) / VIRUS LIKE PARTICLE (ウイルス様粒子) / Nodaviridae / shrimp nodavirus