EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity.
ジャーナル・号・ページ	PLoS Pathog, Vol. 9, Issue 10, Page e1003690, Year 2013
掲載日	2013年10月10日
著者	Kwangkook Lee / Shenyan Gu / Lei Jin / Thi Tuc Nghi Le / Luisa W Cheng / Jasmin Strotmeier / Anna Magdalena Kruel / Guorui Yao / Kay Perry / Andreas Rummel / Rongsheng Jin /
PubMed 要旨	Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the fatal disease botulism, a flaccid paralysis of the muscle. BoNTs are released together with several auxiliary ...Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the fatal disease botulism, a flaccid paralysis of the muscle. BoNTs are released together with several auxiliary proteins as progenitor toxin complexes (PTCs) to become highly potent oral poisons. Here, we report the structure of a ∼760 kDa 14-subunit large PTC of serotype A (L-PTC/A) and reveal insight into its absorption mechanism. Using a combination of X-ray crystallography, electron microscopy, and functional studies, we found that L-PTC/A consists of two structurally and functionally independent sub-complexes. A hetero-dimeric 290 kDa complex protects BoNT, while a hetero-dodecameric 470 kDa complex facilitates its absorption in the harsh environment of the gastrointestinal tract. BoNT absorption is mediated by nine glycan-binding sites on the dodecameric sub-complex that forms multivalent interactions with carbohydrate receptors on intestinal epithelial cells. We identified monosaccharides that blocked oral BoNT intoxication in mice, which suggests a new strategy for the development of preventive countermeasures for BoNTs based on carbohydrate receptor mimicry.
リンク	PLoS Pathog / PubMed:24130488 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.055 - 30.0 Å
構造データ	EMDB-2416: Negative staining 3D-EM of the HA complex of Botulinum toxin type A 手法: EM (単粒子) / 解像度: 30.0 Å EMDB-2417: Negative staining 3D-EM of the progenitor toxin complex (PTC) of Botulinum toxin type A 手法: EM (単粒子) / 解像度: 30.0 Å PDB-4lo0: Apo HA17-HA33 手法: X-RAY DIFFRACTION / 解像度: 2.055 Å PDB-4lo1: HA17-HA33-Gal 手法: X-RAY DIFFRACTION / 解像度: 2.254 Å PDB-4lo2: HA17-HA33-Lac 手法: X-RAY DIFFRACTION / 解像度: 2.254 Å PDB-4lo3: HA17-HA33-LacNac 手法: X-RAY DIFFRACTION / 解像度: 2.249 Å PDB-4lo4: Apo HA-70 手法: X-RAY DIFFRACTION / 解像度: 2.871 Å PDB-4lo5: HA70-alpha2,3-SiaLC 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-4lo6: HA70-alpha2,6-SiaLC 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å PDB-4lo7: HA70(D3)-HA17-HA33 手法: X-RAY DIFFRACTION / 解像度: 3.73 Å PDB-4lo8: HA70(D3)-HA17 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å
化合物	ChemComp-HOH: WATER ChemComp-GAL: beta-D-galactopyranose / β-D-ガラクトピラノ-ス ChemComp-CL: Unknown entry / クロリド
由来	clostridium botulinum (ボツリヌス菌)
キーワード	PROTEIN TRANSPORT / progenitor toxin complex / botulinum neurotoxin / botulism / neurotoxin associated protein / hemagglutinin / carbohydrate/sugar binding / secreted protein