EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic insights into the recycling machine of the SNARE complex.
ジャーナル・号・ページ	Nature, Vol. 518, Issue 7537, Page 61-67, Year 2015
掲載日	2015年2月5日
著者	Minglei Zhao / Shenping Wu / Qiangjun Zhou / Sandro Vivona / Daniel J Cipriano / Yifan Cheng / Axel T Brunger /
PubMed 要旨	Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. The ATPase NSF (N- ...Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. The ATPase NSF (N-ethylmaleimide sensitive factor), together with SNAPs (soluble NSF attachment protein), disassembles the SNARE complex into its protein components, making individual SNAREs available for subsequent rounds of fusion. Here we report structures of ATP- and ADP-bound NSF, and the NSF/SNAP/SNARE (20S) supercomplex determined by single-particle electron cryomicroscopy at near-atomic to sub-nanometre resolution without imposing symmetry. Large, potentially force-generating, conformational differences exist between ATP- and ADP-bound NSF. The 20S supercomplex exhibits broken symmetry, transitioning from six-fold symmetry of the NSF ATPase domains to pseudo four-fold symmetry of the SNARE complex. SNAPs interact with the SNARE complex with an opposite structural twist, suggesting an unwinding mechanism. The interfaces between NSF, SNAPs, and SNAREs exhibit characteristic electrostatic patterns, suggesting how one NSF/SNAP species can act on many different SNARE complexes.
リンク	Nature / PubMed:25581794 / PubMed Central
手法	EM (単粒子)
解像度	4.2 - 8.4 Å
構造データ	6204 3j94 EMDB-6204, PDB-3j94: Structure of ATP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopy 手法: EM (単粒子) / 解像度: 4.2 Å 6205 3j95 EMDB-6205, PDB-3j95: Structure of ADP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopy 手法: EM (単粒子) / 解像度: 7.6 Å 6206 3j96 EMDB-6206: Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state I PDB-3j96: Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I) 手法: EM (単粒子) / 解像度: 7.6 Å 6207 3j97 EMDB-6207: Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state II PDB-3j97: Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State II) 手法: EM (単粒子) / 解像度: 7.8 Å 6208 3j98 EMDB-6208: Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state IIIa PDB-3j98: Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIa) 手法: EM (単粒子) / 解像度: 8.4 Å 6209 3j99 EMDB-6209: Structure of 20S supercomplex determined by single particle cryoelectron microscopy, state IIIb PDB-3j99: Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIb) 手法: EM (単粒子) / 解像度: 8.2 Å EMDB-6210: Structure of 20S supercomplex with V7-SNARE determined by single particle cryoelectron microscopy 手法: EM (単粒子) / 解像度: 8.0 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	cricetulus griseus (モンゴルキヌゲネズミ) rattus norvegicus (ドブネズミ)
キーワード	HYDROLASE / ATPases associated with diverse cellular activities / vesicle trafficking