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-Structure paper
タイトル | Conformational changes in tubulin in GMPCPP and GDP-taxol microtubules observed by cryoelectron microscopy. |
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ジャーナル・号・ページ | J Cell Biol, Vol. 198, Issue 3, Page 315-322, Year 2012 |
掲載日 | 2012年8月6日 |
著者 | Hiroaki Yajima / Toshihiko Ogura / Ryo Nitta / Yasushi Okada / Chikara Sato / Nobutaka Hirokawa / |
PubMed 要旨 | Microtubules are dynamic polymers that stochastically switch between growing and shrinking phases. Microtubule dynamics are regulated by guanosine triphosphate (GTP) hydrolysis by β-tubulin, but the ...Microtubules are dynamic polymers that stochastically switch between growing and shrinking phases. Microtubule dynamics are regulated by guanosine triphosphate (GTP) hydrolysis by β-tubulin, but the mechanism of this regulation remains elusive because high-resolution microtubule structures have only been revealed for the guanosine diphosphate (GDP) state. In this paper, we solved the cryoelectron microscopy (cryo-EM) structure of microtubule stabilized with a GTP analogue, guanylyl 5'-α,β-methylenediphosphonate (GMPCPP), at 8.8-Å resolution by developing a novel cryo-EM image reconstruction algorithm. In contrast to the crystal structures of GTP-bound tubulin relatives such as γ-tubulin and bacterial tubulins, significant changes were detected between GMPCPP and GDP-taxol microtubules at the contacts between tubulins both along the protofilament and between neighboring protofilaments, contributing to the stability of the microtubule. These findings are consistent with the structural plasticity or lattice model and suggest the structural basis not only for the regulatory mechanism of microtubule dynamics but also for the recognition of the nucleotide state of the microtubule by several microtubule-binding proteins, such as EB1 or kinesin. |
リンク | J Cell Biol / PubMed:22851320 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 8.9 Å |
構造データ | |
化合物 | ChemComp-MG: ChemComp-GTP: |
由来 |
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キーワード | STRUCTURAL PROTEIN / Microtubule / Tubulin / GTP-state structure / GMPCPP / Microtubule stabilaization / Micotubule polymerization |