EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A refined model of the prototypical Salmonella SPI-1 T3SS basal body reveals the molecular basis for its assembly.
ジャーナル・号・ページ	PLoS Pathog, Vol. 9, Issue 4, Page e1003307, Year 2013
掲載日	2013年4月25日
著者	Julien R C Bergeron / Liam J Worrall / Nikolaos G Sgourakis / Frank DiMaio / Richard A Pfuetzner / Heather B Felise / Marija Vuckovic / Angel C Yu / Samuel I Miller / David Baker / Natalie C J Strynadka /
PubMed 要旨	The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed ...The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a "basal body", a lock-nut structure spanning both bacterial membranes, and a "needle" that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism.
リンク	PLoS Pathog / PubMed:23633951 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.801 - 11.7 Å
構造データ	PDB-3j1v: A refined model of the prototypical Salmonella typhimurium T3SS basal body reveals the molecular basis for its assembly 手法: ELECTRON MICROSCOPY / 解像度: 11.7 Å PDB-3j1w: A refined model of the prototypical Salmonella typhimurium T3SS basal body reveals the molecular basis for its assembly 手法: ELECTRON MICROSCOPY / 解像度: 11.7 Å PDB-3j1x: A refined model of the prototypical Salmonella typhimurium T3SS basal body reveals the molecular basis for its assembly 手法: ELECTRON MICROSCOPY / 解像度: 11.7 Å PDB-4g08: Crystal structure of the periplasmic domain of InvG 手法: X-RAY DIFFRACTION / 解像度: 1.801 Å PDB-4g1i: Structure of the PrgH periplasmic domain 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-4g2s: Crystal structure of a Salmonella type III secretion system protein 手法: X-RAY DIFFRACTION / 解像度: 1.858 Å
化合物	ChemComp-HOH: WATER ChemComp-1PE: PENTAETHYLENE GLYCOL / ペンタエチレングリコ-ル / 沈殿剤YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-CA*: Unknown entry / カルシウムジカチオン
由来	salmonella enterica subsp. enterica serovar typhimurium (サルモネラ菌)
キーワード	CELL INVASION / T3SS / Secretin / Ring-building motif / protein secretion / PrgH / Type III secretion / InvG / FHA domain