タイトル | Directed evolution and structural characterization of a simvastatin synthase |
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ジャーナル・号・ページ | Chem. Biol., Vol. 16, Page 1064-1074, Year 2009 |
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掲載日 | 2009年5月27日 (構造データの登録日) |
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著者 | Gao, X. / Xie, X. / Pashkov, I. / Sawaya, M.R. / Laidman, J. / Zhang, W. / Cacho, R. / Yeates, T.O. / Tang, Y. |
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リンク | Chem. Biol. / PubMed:19875080 |
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手法 | X線回折 |
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解像度 | 2 - 3.4 Å |
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構造データ | PDB-3hl9: Simvastatin Synthase (LovD) from Aspergillus terreus, unliganded 手法: X-RAY DIFFRACTION / 解像度: 3.4 Å PDB-3hlb: Simvastatin Synthase (LovD) from Aspergillus terreus, unliganded, selenomethionyl derivative 手法: X-RAY DIFFRACTION / 解像度: 2.5 Å PDB-3hlc: Simvastatin Synthase (LovD) from Aspergillus terreus, S5 mutant, unliganded 手法: X-RAY DIFFRACTION / 解像度: 2 Å PDB-3hld: Simvastatin Synthase (LovD), from Aspergillus terreus, S5 mutant complex with monacolin J acid 手法: X-RAY DIFFRACTION / 解像度: 2 Å PDB-3hle: Simvastatin Synthase (LovD), from Aspergillus terreus, S5 mutant, S76A mutant, complex with monacolin J acid 手法: X-RAY DIFFRACTION / 解像度: 2.06 Å PDB-3hlf: Simvastatin Synthase (LovD), from Aspergillus terreus, S5 mutant, S76A mutant, complex with simvastatin 手法: X-RAY DIFFRACTION / 解像度: 2 Å PDB-3hlg: Simvastatin Synthase (LovD), from Aspergillus terreus, S5 mutant, S76A mutant, complex with lovastatin 手法: X-RAY DIFFRACTION / 解像度: 2.01 Å |
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化合物 | ChemComp-MJA: (3R,5R)-3,5-dihydroxy-7-[(1S,2S,6R,8S,8aR)-8-hydroxy-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]heptanoic acid
ChemComp-LVA: (3R,5R)-7-((1R,2R,6S,8R,8AS)-2,6-DIMETHYL-8-{[(2R)-2-METHYLBUTANOYL]OXY}-1,2,6,7,8,8A-HEXAHYDRONAPHTHALEN-1-YL)-3,5-DIHYDROXYHEPTANOIC ACID / ロバスタチン酸
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由来 | - aspergillus terreus (カビ)
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キーワード | TRANSFERASE / alpha/beta hydrolase fold |
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