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-Structure paper
タイトル | The crystal structure of coxsackievirus A21 and its interaction with ICAM-1. |
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ジャーナル・号・ページ | Structure, Vol. 13, Issue 7, Page 1019-1033, Year 2005 |
掲載日 | 2005年12月9日 |
著者 | Chuan Xiao / Carol M Bator-Kelly / Elizabeth Rieder / Paul R Chipman / Alister Craig / Richard J Kuhn / Eckard Wimmer / Michael G Rossmann / |
PubMed 要旨 | CVA21 and polioviruses both belong to the Enterovirus genus in the family of Picornaviridae, whereas rhinoviruses form a distinct picornavirus genus. Nevertheless, CVA21 and the major group of human ...CVA21 and polioviruses both belong to the Enterovirus genus in the family of Picornaviridae, whereas rhinoviruses form a distinct picornavirus genus. Nevertheless, CVA21 and the major group of human rhinoviruses recognize intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor, whereas polioviruses use poliovirus receptor. The crystal structure of CVA21 has been determined to 3.2 A resolution. Its structure has greater similarity to poliovirus structures than to other known picornavirus structures. Cryo-electron microscopy (cryo-EM) was used to determine an 8.0 A resolution structure of CVA21 complexed with an ICAM-1 variant, ICAM-1(Kilifi). The cryo-EM map was fitted with the crystal structures of ICAM-1 and CVA21. Significant differences in the structure of CVA21 with respect to the poliovirus structures account for the inability of ICAM-1 to bind polioviruses. The interface between CVA21 and ICAM-1 has shape and electrostatic complementarity with many residues being conserved among those CVAs that bind ICAM-1. |
リンク | Structure / PubMed:16004874 |
手法 | EM (単粒子) / X線回折 |
解像度 | 3.2 - 8.0 Å |
構造データ | EMDB-1114: The crystal structure of coxsackievirus A21 and its interaction with ICAM-1. PDB-1z7s: |
化合物 | ChemComp-CA: ChemComp-MYR: ChemComp-5GP: ChemComp-HOH: ChemComp-NAG: |
由来 |
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キーワード | VIRUS / PICORNAVIRUS / Coxsackievirus / A21 / Capsid Protein / Viral Protein / Icosahedral virus / Virus/Receptor / ICAM-1 / Kilifi / CD54 / Human Coxsackievirus A21 / virus-receptor complex |