EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.
ジャーナル・号・ページ	Elife, Vol. 8, Year 2019
掲載日	2019年6月18日
著者	Jean Paul Armache / Nathan Gamarra / Stephanie L Johnson / John D Leonard / Shenping Wu / Geeta J Narlikar / Yifan Cheng /
PubMed 要旨	The SNF2h remodeler slides nucleosomes most efficiently as a dimer, yet how the two protomers avoid a tug-of-war is unclear. Furthermore, SNF2h couples histone octamer deformation to nucleosome ...The SNF2h remodeler slides nucleosomes most efficiently as a dimer, yet how the two protomers avoid a tug-of-war is unclear. Furthermore, SNF2h couples histone octamer deformation to nucleosome sliding, but the underlying structural basis remains unknown. Here we present cryo-EM structures of SNF2h-nucleosome complexes with ADP-BeF that capture two potential reaction intermediates. In one structure, histone residues near the dyad and in the H2A-H2B acidic patch, distal to the active SNF2h protomer, appear disordered. The disordered acidic patch is expected to inhibit the second SNF2h protomer, while disorder near the dyad is expected to promote DNA translocation. The other structure doesn't show octamer deformation, but surprisingly shows a 2 bp translocation. FRET studies indicate that ADP-BeF predisposes SNF2h-nucleosome complexes for an elemental translocation step. We propose a model for allosteric control through the nucleosome, where one SNF2h protomer promotes asymmetric octamer deformation to inhibit the second protomer, while stimulating directional DNA translocation.
リンク	Elife / PubMed:31210637 / PubMed Central
手法	EM (単粒子)
解像度	3.39 - 8.4 Å
構造データ	EMDB-9351: Cryo-EM structure of singly-bound SNF2h-nucleosome complex, with SNF2h bound at SHL+2 手法: EM (単粒子) / 解像度: 8.4 Å EMDB-9352: Cryo-EM structure of singly-bound SNF2h-nucleosome complex at 3.4 A 手法: EM (単粒子) / 解像度: 3.39 Å EMDB-9353: Cryo-EM structure of SNF2h doubly-bound to the nucleosome 手法: EM (単粒子) / 解像度: 8.4 Å EMDB-9354: Cryo-EM structure of singly-bound SNF2h-nucleosome complex at 3.9A with SNF2h bound at SHL-2 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-9355: Cryo-EM structure of singly-bound SNF2h-nucleosome complex at 6.9A with SNF2h bound at SHL+2 手法: EM (単粒子) / 解像度: 6.9 Å 9356 6ne3 EMDB-9356: Cryo-EM structure of singly-bound SNF2h-nucleosome complex with SNF2h bound at the flanking DNA proximal side PDB-6ne3: Cryo-EM structure of singly-bound SNF2h-nucleosome complex with SNF2h bound at SHL-2 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM
由来	xenopus laevis (アフリカツメガエル) homo sapiens (ヒト)
キーワード	DNA BINDING PROTEIN/DNA / ISWI / Chromatin / Nucleosome / DNA / SNF2h / histones / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex