EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of translationally inactive mammalian ribosomes.
ジャーナル・号・ページ	Elife, Vol. 7, Year 2018
掲載日	2018年10月24日
著者	Alan Brown / Matthew R Baird / Matthew Cj Yip / Jason Murray / Sichen Shao /
PubMed 要旨	The cellular levels and activities of ribosomes directly regulate gene expression during numerous physiological processes. The mechanisms that globally repress translation are incompletely understood. ...The cellular levels and activities of ribosomes directly regulate gene expression during numerous physiological processes. The mechanisms that globally repress translation are incompletely understood. Here, we use electron cryomicroscopy to analyze inactive ribosomes isolated from mammalian reticulocytes, the penultimate stage of red blood cell differentiation. We identify two types of ribosomes that are translationally repressed by protein interactions. The first comprises ribosomes sequestered with elongation factor 2 (eEF2) by SERPINE mRNA binding protein 1 (SERBP1) occupying the ribosomal mRNA entrance channel. The second type are translationally repressed by a novel ribosome-binding protein, interferon-related developmental regulator 2 (IFRD2), which spans the P and E sites and inserts a C-terminal helix into the mRNA exit channel to preclude translation. IFRD2 binds ribosomes with a tRNA occupying a noncanonical binding site, the 'Z site', on the ribosome. These structures provide functional insights into how ribosomal interactions may suppress translation to regulate gene expression.
リンク	Elife / PubMed:30355441 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 5.9 Å
構造データ	EMDB-9234: Rabbit 80S ribosome with a P-site tRNA (unrotated state) 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-9235: Rabbit 80S ribosome with a P- and E-site tRNA 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-9236: Rabbit 80S ribosome with a Z-site tRNA (unrotated state) 手法: EM (単粒子) / 解像度: 4.2 Å 9237 6mtb EMDB-9237, PDB-6mtb: Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) 手法: EM (単粒子) / 解像度: 3.6 Å 9239 6mtc EMDB-9239, PDB-6mtc: Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) 手法: EM (単粒子) / 解像度: 3.4 Å 9240 6mtd EMDB-9240, PDB-6mtd: Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-9241: Rabbit 80S ribosome with A/P and P/E tRNAs (rotated state) 手法: EM (単粒子) / 解像度: 3.8 Å 9242 6mte EMDB-9242, PDB-6mte: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM
由来	oryctolagus cuniculus (ウサギ) unidentified (未定義) Rabbit (ウサギ) Bovine (ウシ)
キーワード	RIBOSOME / translation