EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A structural model of flagellar filament switching across multiple bacterial species.
ジャーナル・号・ページ	Nat Commun, Vol. 8, Issue 1, Page 960, Year 2017
掲載日	2017年10月16日
著者	Fengbin Wang / Andrew M Burrage / Sandra Postel / Reece E Clark / Albina Orlova / Eric J Sundberg / Daniel B Kearns / Edward H Egelman /
PubMed 要旨	The bacterial flagellar filament has long been studied to understand how a polymer composed of a single protein can switch between different supercoiled states with high cooperativity. Here we ...The bacterial flagellar filament has long been studied to understand how a polymer composed of a single protein can switch between different supercoiled states with high cooperativity. Here we present near-atomic resolution cryo-EM structures for flagellar filaments from both Gram-positive Bacillus subtilis and Gram-negative Pseudomonas aeruginosa. Seven mutant flagellar filaments in B. subtilis and two in P. aeruginosa capture two different states of the filament. These reliable atomic models of both states reveal conserved molecular interactions in the interior of the filament among B. subtilis, P. aeruginosa and Salmonella enterica. Using the detailed information about the molecular interactions in two filament states, we successfully predict point mutations that shift the equilibrium between those two states. Further, we observe the dimerization of P. aeruginosa outer domains without any perturbation of the conserved interior of the filament. Our results give new insights into how the flagellin sequence has been "tuned" over evolution.Bacterial flagellar filaments are composed almost entirely of a single protein-flagellin-which can switch between different supercoiled states in a highly cooperative manner. Here the authors present near-atomic resolution cryo-EM structures of nine flagellar filaments, and begin to shed light on the molecular basis of filament switching.
リンク	Nat Commun / PubMed:29038601 / PubMed Central
手法	EM (らせん対称)
解像度	3.8 - 6.7 Å
構造データ	8847 5wjt EMDB-8847, PDB-5wjt: Cryo-EM structure of B. subtilis flagellar filaments N226Y 手法: EM (らせん対称) / 解像度: 3.8 Å 8848 5wju EMDB-8848: Cryo-EM structure of B. subtilis flagellar filaments A39VN133H PDB-5wju: Cryo-EM structure of B. subtilis flagellar filaments A39V, N133H 手法: EM (らせん対称) / 解像度: 4.3 Å 8849 5wjv EMDB-8849, PDB-5wjv: Cryo-EM structure of B. subtilis flagellar filaments A233V 手法: EM (らせん対称) / 解像度: 5.5 Å 8850 5wjw EMDB-8850, PDB-5wjw: Cryo-EM structure of B. subtilis flagellar filaments H84R 手法: EM (らせん対称) / 解像度: 4.4 Å 8851 5wjx EMDB-8851, PDB-5wjx: Cryo-EM structure of B. subtilis flagellar filaments S17P 手法: EM (らせん対称) / 解像度: 6.7 Å 8852 5wjy EMDB-8852, PDB-5wjy: Cryo-EM structure of B. subtilis flagellar filaments S285P 手法: EM (らせん対称) / 解像度: 4.5 Å 8853 5wjz EMDB-8853, PDB-5wjz: Cryo-EM structure of B. subtilis flagellar filaments E115G 手法: EM (らせん対称) / 解像度: 5.7 Å 8855 5wk5 EMDB-8855, PDB-5wk5: Cryo-EM structure of P. aeruginosa flagellar filaments A443V 手法: EM (らせん対称) / 解像度: 4.2 Å 8856 5wk6 EMDB-8856, PDB-5wk6: Cryo-EM structure of P. aeruginosa flagellar filaments G420A 手法: EM (らせん対称) / 解像度: 4.3 Å
由来	bacillus subtilis (枯草菌) Pseudomonas aeruginosa PAO1 (緑膿菌) pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (緑膿菌)
キーワード	PROTEIN FIBRIL / bacteria flagella / helical polymers / cryo-EM (低温電子顕微鏡法)