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-Structure paper
タイトル | Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex. |
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ジャーナル・号・ページ | Cell, Vol. 166, Issue 6, Page 1411-1422.e16, Year 2016 |
掲載日 | 2016年9月8日 |
著者 | Philip J Robinson / Michael J Trnka / David A Bushnell / Ralph E Davis / Pierre-Jean Mattei / Alma L Burlingame / Roger D Kornberg / |
PubMed 要旨 | A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass ...A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription. |
リンク | Cell / PubMed:27610567 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 15.3 - 21.9 Å |
構造データ | EMDB-8305: Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail (Focused Refinement) EMDB-8307: EMDB-8308: |
化合物 | ChemComp-ZN: ChemComp-MG: |
由来 |
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キーワード | TRANSCRIPTION / TRANSFERASE/DNA / Transcriptional Initiation / Mediator / Pre-Initiation Complex / Carboxy-terminal domain (CTD) / TRANSFERASE-DNA complex |