+検索条件
-Structure paper
タイトル | Arrangement of the Polymerase Complexes inside a Nine-Segmented dsRNA Virus. |
---|---|
ジャーナル・号・ページ | Structure, Vol. 28, Issue 6, Page 604-612.e3, Year 2020 |
掲載日 | 2020年6月2日 |
著者 | Jason T Kaelber / Wen Jiang / Scott C Weaver / Albert J Auguste / Wah Chiu / |
PubMed 要旨 | Members of the family Reoviridae package several copies of the viral polymerase complex into their capsid to carry out replication and transcription within viral particles. Classical single-particle ...Members of the family Reoviridae package several copies of the viral polymerase complex into their capsid to carry out replication and transcription within viral particles. Classical single-particle reconstruction encounters difficulties resolving structures such as the intraparticle polymerase complex because refinement can converge to an incorrect map and because the map could depict a nonrepresentative subset of particles or an average of heterogeneous particles. Using the nine-segmented Fako virus, we tested hypotheses for the arrangement and number of polymerase complexes within the virion by measuring how well each hypothesis describes the set of cryoelectron microscopy images of individual viral particles. We find that the polymerase complex in Fako virus binds at ten possible sites despite having only nine genome segments. A single asymmetric configuration describes the arrangement of these complexes in both virions and genome-free capsids. Similarities between the arrangements of Reoviridae with 9, 10, and 11 segments indicate the generalizability of this architecture. |
リンク | Structure / PubMed:32049031 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.9 - 11.4 Å |
構造データ | EMDB-7941: EMDB-7944: Fako virus full particles, icosahedral reconstruction EMDB-7945: EMDB-7948: EMDB-7949: EMDB-7953: EMDB-7954: |
由来 |
|
キーワード | VIRUS / capsid / virion / Reoviridae / T=2 |