EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 115, Issue 6, Page 1292-1297, Year 2018
掲載日	2018年2月6日
著者	Ahmet Mentes / Andrew Huehn / Xueqi Liu / Adam Zwolak / Roberto Dominguez / Henry Shuman / E Michael Ostap / Charles V Sindelar /
PubMed 要旨	Myosins adjust their power outputs in response to mechanical loads in an isoform-dependent manner, resulting in their ability to dynamically adapt to a range of motile challenges. Here, we reveal the ...Myosins adjust their power outputs in response to mechanical loads in an isoform-dependent manner, resulting in their ability to dynamically adapt to a range of motile challenges. Here, we reveal the structural basis for force-sensing based on near-atomic resolution structures of one rigor and two ADP-bound states of myosin-IB (myo1b) bound to actin, determined by cryo-electron microscopy. The two ADP-bound states are separated by a 25° rotation of the lever. The lever of the first ADP state is rotated toward the pointed end of the actin filament and forms a previously unidentified interface with the N-terminal subdomain, which constitutes the upper half of the nucleotide-binding cleft. This pointed-end orientation of the lever blocks ADP release by preventing the N-terminal subdomain from the pivoting required to open the nucleotide binding site, thus revealing how myo1b is inhibited by mechanical loads that restrain lever rotation. The lever of the second ADP state adopts a rigor-like orientation, stabilized by class-specific elements of myo1b. We identify a role for this conformation as an intermediate in the ADP release pathway. Moreover, comparison of our structures with other myosins reveals structural diversity in the actomyosin binding site, and we reveal the high-resolution structure of actin-bound phalloidin, a potent stabilizer of filamentous actin. These results provide a framework to understand the spectrum of force-sensing capacities among the myosin superfamily.
リンク	Proc Natl Acad Sci U S A / PubMed:29358376 / PubMed Central
手法	EM (らせん対称) / X線回折
解像度	3.141 - 3.9 Å
構造データ	7329 6c1d EMDB-7329, PDB-6c1d: High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing 手法: EM (らせん対称) / 解像度: 3.2 Å 7330 6c1g EMDB-7330, PDB-6c1g: High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing 手法: EM (らせん対称) / 解像度: 3.8 Å 7331 6c1h EMDB-7331, PDB-6c1h: High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing 手法: EM (らせん対称) / 解像度: 3.9 Å PDB-5v7x: Crystal Structure of Myosin 1b residues 1-728 with bound sulfate and Calmodulin 手法: X-RAY DIFFRACTION / 解像度: 3.141 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	unidentified (未定義) Rabbit (ウサギ) Rat (クマネズミ属) death cap (タマゴテングタケ) rattus norvegicus (ドブネズミ) oryctolagus cuniculus (ウサギ) homo sapiens (ヒト) amanita phalloides (タマゴテングタケ)
キーワード	MOTOR PROTEIN (モータータンパク質) / myosin (ミオシン) / motor (機関 (機械)) / calmodulin (カルモジュリン) / unconventional (慣習) / actin (アクチン) / force (力 (物理学)) / STRUCTURAL PROTEIN (タンパク質) / Mechanochemistry / Mechanobiology / Structural Biology (構造生物学) / Cytoskeleton (細胞骨格) / Molecular Motor (分子モーター) / Myosin-I (ミオシン)