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-Structure paper
タイトル | Electron cryotomography analysis of Dam1C/DASH at the kinetochore-spindle interface in situ. |
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ジャーナル・号・ページ | J Cell Biol, Vol. 218, Issue 2, Page 455-473, Year 2019 |
掲載日 | 2019年2月4日 |
著者 | Cai Tong Ng / Li Deng / Chen Chen / Hong Hwa Lim / Jian Shi / Uttam Surana / Lu Gan / |
PubMed 要旨 | In dividing cells, depolymerizing spindle microtubules move chromosomes by pulling at their kinetochores. While kinetochore subcomplexes have been studied extensively in vitro, little is known about ...In dividing cells, depolymerizing spindle microtubules move chromosomes by pulling at their kinetochores. While kinetochore subcomplexes have been studied extensively in vitro, little is known about their in vivo structure and interactions with microtubules or their response to spindle damage. Here we combine electron cryotomography of serial cryosections with genetic and pharmacological perturbation to study the yeast chromosome segregation machinery in vivo. Each kinetochore microtubule has one (rarely, two) Dam1C/DASH outer kinetochore assemblies. Dam1C/DASH contacts the microtubule walls and does so with its flexible "bridges"; there are no contacts with the protofilaments' curved tips. In metaphase, ∼40% of the Dam1C/DASH assemblies are complete rings; the rest are partial rings. Ring completeness and binding position along the microtubule are sensitive to kinetochore attachment and tension, respectively. Our study and those of others support a model in which each kinetochore must undergo cycles of conformational change to couple microtubule depolymerization to chromosome movement. |
リンク | J Cell Biol / PubMed:30504246 / PubMed Central |
手法 | EM (トモグラフィー) |
解像度 | 32.0 Å |
構造データ | EMDB-6912: EMDB-6914: |
由来 |
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