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-Structure paper
タイトル | Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains. |
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ジャーナル・号・ページ | Nat Commun, Vol. 8, Page 15092, Year 2017 |
掲載日 | 2017年4月10日 |
![]() | Yuan Yuan / Duanfang Cao / Yanfang Zhang / Jun Ma / Jianxun Qi / Qihui Wang / Guangwen Lu / Ying Wu / Jinghua Yan / Yi Shi / Xinzheng Zhang / George F Gao / ![]() |
PubMed 要旨 | The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and ...The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies. |
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手法 | EM (単粒子) / X線回折 |
解像度 | 1.5 - 4.2 Å |
構造データ | EMDB-6703, PDB-5x58: EMDB-6704, PDB-5x59: EMDB-6705, PDB-5x5b: EMDB-6706, PDB-5x5c: EMDB-6707, PDB-5x5f: ![]() PDB-5x4r: ![]() PDB-5x4s: |
化合物 | ![]() ChemComp-HOH: ![]() ChemComp-NAG: |
由来 |
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![]() | VIRAL PROTEIN / MERS-CoV / spike / N-terminal domain / SARS-CoV / spike glycoprotein / prefusion / single particle |