EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains.
ジャーナル・号・ページ	Nat Commun, Vol. 8, Page 15092, Year 2017
掲載日	2017年4月10日
著者	Yuan Yuan / Duanfang Cao / Yanfang Zhang / Jun Ma / Jianxun Qi / Qihui Wang / Guangwen Lu / Ying Wu / Jinghua Yan / Yi Shi / Xinzheng Zhang / George F Gao /
PubMed 要旨	The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and ...The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies.
リンク	Nat Commun / PubMed:28393837 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.5 - 4.2 Å
構造データ	6703 5x58 EMDB-6703, PDB-5x58: Prefusion structure of SARS-CoV spike glycoprotein, conformation 1 手法: EM (単粒子) / 解像度: 3.2 Å 6704 5x59 EMDB-6704, PDB-5x59: Prefusion structure of MERS-CoV spike glycoprotein, three-fold symmetry 手法: EM (単粒子) / 解像度: 3.7 Å 6705 5x5b EMDB-6705, PDB-5x5b: Prefusion structure of SARS-CoV spike glycoprotein, conformation 2 手法: EM (単粒子) / 解像度: 3.7 Å 6706 5x5c EMDB-6706, PDB-5x5c: Prefusion structure of MERS-CoV spike glycoprotein, conformation 1 手法: EM (単粒子) / 解像度: 4.1 Å 6707 5x5f EMDB-6707, PDB-5x5f: Prefusion structure of MERS-CoV spike glycoprotein, conformation 2 手法: EM (単粒子) / 解像度: 4.2 Å PDB-5x4r: Structure of the N-terminal domain (NTD) of MERS-CoV spike protein 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å PDB-5x4s: Structure of the N-terminal domain (NTD)of SARS-CoV spike protein 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å
化合物	ChemComp-HOH: WATER / 水 ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	sars coronavirus bj01 (SARSコロナウイルス) middle east respiratory syndrome coronavirus (MERSコロナウイルス) human sars coronavirus (SARSコロナウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / MERS-CoV (MERSコロナウイルス) / spike / N-terminal domain (N末端) / SARS-CoV (SARSコロナウイルス) / spike glycoprotein (スパイクタンパク質) / prefusion / single particle (単粒子解析法)