EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.
ジャーナル・号・ページ	Cell, Vol. 162, Issue 4, Page 849-859, Year 2015
掲載日	2015年8月13日
著者	Rui Zhang / Gregory M Alushin / Alan Brown / Eva Nogales /
PubMed 要旨	Microtubule (MT) dynamic instability is driven by GTP hydrolysis and regulated by microtubule-associated proteins, including the plus-end tracking end-binding protein (EB) family. We report six cryo- ...Microtubule (MT) dynamic instability is driven by GTP hydrolysis and regulated by microtubule-associated proteins, including the plus-end tracking end-binding protein (EB) family. We report six cryo-electron microscopy (cryo-EM) structures of MTs, at 3.5 Å or better resolution, bound to GMPCPP, GTPγS, or GDP, either decorated with kinesin motor domain after polymerization or copolymerized with EB3. Subtle changes around the E-site nucleotide during hydrolysis trigger conformational changes in α-tubulin around an "anchor point," leading to global lattice rearrangements and strain generation. Unlike the extended lattice of the GMPCPP-MT, the EB3-bound GTPγS-MT has a compacted lattice that differs in lattice twist from that of the also compacted GDP-MT. These results and the observation that EB3 promotes rapid hydrolysis of GMPCPP suggest that EB proteins modulate structural transitions at growing MT ends by recognizing and promoting an intermediate state generated during GTP hydrolysis. Our findings explain both EBs end-tracking behavior and their effect on microtubule dynamics.
リンク	Cell / PubMed:26234155 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	3.3 - 4.4 Å
構造データ	EMDB-6347: Cryo-EM structure of GTPgammaS microtubule co-polymerized with EB3 手法: EM (らせん対称) / 解像度: 3.4 Å EMDB-6348: Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 and decorated with kinesin 手法: EM (らせん対称) / 解像度: 3.5 Å 6349 3jak EMDB-6349, PDB-3jak: Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound) 手法: EM (らせん対称) / 解像度: 3.3 Å 6350 3jal EMDB-6350, PDB-3jal: Cryo-EM structure of GMPCPP-microtubule co-polymerized with EB3 手法: EM (らせん対称) / 解像度: 3.5 Å 6351 3jar EMDB-6351, PDB-3jar: Cryo-EM structure of GDP-microtubule co-polymerized with EB3 手法: EM (らせん対称) / 解像度: 3.4 Å 6352 3jat EMDB-6352, PDB-3jat: Cryo-EM structure of GMPCPP-microtubule (14 protofilaments) decorated with kinesin 手法: EM (らせん対称) / 解像度: 3.5 Å 6353 3jas EMDB-6353, PDB-3jas: Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin 手法: EM (らせん対称) / 解像度: 3.5 Å 6354 3jaw EMDB-6354: Asymmetric (C1) reconstruction of EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP and GDP) PDB-3jaw: Atomic model of a microtubule seam based on a cryo-EM reconstruction of the EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-6355: Asymmetric (C1) reconstruction of GMPCPP-microtubule (13 protofilaments) decorated with kinesin 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GSP: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / GTP-γ-S ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM / グアノシン二リン酸
由来	sus scrofa (ブタ) homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN (タンパク質) / microtubule (微小管) / EB3 / GTPgammaS / kinesin (キネシン) / GMPCPP / GDP / seam