EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic insights into E. coli recovery from growth arrest.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2026年4月11日
著者	Ahmed H Hassan / Yuko Nakano / Howard Gamper / Isao Masuda / Ludmila Vesela / Matyas Pinkas / Sathya Narayanan Nagarajan / Jonathan Dworkin / Gregor Blaha / Ya-Ming Hou / Gabriel Demo /
PubMed 要旨	Bacteria survive hostile conditions by shutting down protein synthesis, but how they restart growth remains poorly understood. Here, we use an E. coli ΔrimM strain, which exhibits a prolonged growth ...Bacteria survive hostile conditions by shutting down protein synthesis, but how they restart growth remains poorly understood. Here, we use an E. coli ΔrimM strain, which exhibits a prolonged growth arrest, as a model to investigate how bacteria recover from this state and restore protein synthesis. RimM is a conserved ribosome maturation factor for the 3'-major (head) domain of the 16S rRNA within the bacterial 30S subunit. The loss of RimM causes a longer delay in recovery than other 30S maturation factors, including RbfA. Cryo-EM analysis of ΔrimM ribosomes suggests a delayed recruitment of ribosomal proteins to the 30S head domain and increased occupancy of the initiation factors IF1 and IF3, as well as recruitment of the silencing factor RsfS to the 50S subunit. These coordinated changes provide a safeguarding mechanism to block the assembly of premature 70S ribosomes. Notably, while the delayed 30S assembly in ΔrimM reduces the activity of global protein synthesis during the recovery phase, bacteria attempt to compensate for this deficiency by producing higher levels of the ribosomal machinery, indicating a programmatic change in energy allocation. These findings highlight the importance of the RimM-assisted assembly of the ribosomal head domain for bacterial recovery from growth arrest.
リンク	Nat Commun / PubMed:41965359
手法	EM (単粒子)
解像度	2.6 - 3.1 Å
構造データ	55171 9ss0 EMDB-55171, PDB-9ss0: 30S ribosomal subunit RimM-KO with IF1 and IF3 (State I) 手法: EM (単粒子) / 解像度: 3.0 Å 55173 9ss1 EMDB-55173, PDB-9ss1: 30S ribosomal subunit RimM-KO with IF1 and IF3 (State II) 手法: EM (単粒子) / 解像度: 2.9 Å 55174 9ss2 EMDB-55174, PDB-9ss2: 30S ribosomal subunit RimM-KO with IF1 and IF3 (State III) 手法: EM (単粒子) / 解像度: 2.9 Å 55176 9ss4 EMDB-55176, PDB-9ss4: pre-50S ribosomal subunit in rimM-KO with RsfS (-H68) 手法: EM (単粒子) / 解像度: 2.7 Å 55177 9ss5 EMDB-55177, PDB-9ss5: 50S ribosomal subunit in rimM-KO with RsfS (+H68) 手法: EM (単粒子) / 解像度: 2.7 Å 55178 9ss6 EMDB-55178, PDB-9ss6: 70S ribosome from RimM-KO 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-55181: 30S ribosomal subunit RimM-WT with IF1 and IF3 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-55182: pre-50S ribosomal subunit from RimM-WT with RsfS (-H68) 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-55183: 50S ribosomal subunit from RimM-WT with RsfS (+H68) 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-55185: 70S ribosome from RimM-WT 手法: EM (単粒子) / 解像度: 3.0 Å
由来	escherichia coli (大腸菌)
キーワード	RIBOSOME / Ribosome assembly / RimM / initiation factor IF1 / initiation factor IF3 / anti-association