EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural studies of ribosome from an anaerobic Bacteroidetes human pathogen Porphyromonas gingivalis.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 53, Issue 10, Year 2025
掲載日	2025年5月22日
著者	Disha-Gajanan Hiregange / Sarit Samiya / Danuta Mizgalska / Efrat Ben-Zeev / Miriam Waghalter / Andre Rivalta / K Shanmugha Rajan / Yehuda Halfon / Elinor Breiner-Goldstein / Igor Kaczmarczyk / Aneta Sroka / Masato Taoka / Yuko Nobe / Toshiaki Isobe / Susanne Paukner / Ella Zimmerman / Anat Bashan / Jan Potempa / Ada Yonath /
PubMed 要旨	Porphyromonas gingivalis, an anaerobic pathogen in chronic periodontitis, belongs to the Bacteroidota phylum and is associated with various virulence factors. Its antibiotic-resistant strains and its ...Porphyromonas gingivalis, an anaerobic pathogen in chronic periodontitis, belongs to the Bacteroidota phylum and is associated with various virulence factors. Its antibiotic-resistant strains and its propensity to form biofilms pose a challenge to effective treatment. To explore therapeutic avenues, we studied the high-resolution cryogenic electron microscope structures of ribosomes from the wild-type P. gingivalis W83 and the macrolide-resistant mutant strain ermΔporN. The structural analysis revealed unique features primarily at the ribosome periphery. Together with the distinctive distribution of ribosomal RNA modifications, these findings offer insights into the therapeutical potential, such as creation of novel therapeutic compounds inhibiting the specific cellular functions of the P. gingivalis ribosomes. Moreover, the high-resolution structure of the ermΔporN ribosome in its complex with the approved antibiotic lefamulin suggests its repurposing against P. gingivalis. Furthermore, we provide a foundation for additional effective strategies to treat periodontitis and associated systemic diseases.
リンク	Nucleic Acids Res / PubMed:40444637 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.1 Å
構造データ	52635 9i5t EMDB-52635, PDB-9i5t: 50S subunit of P. gingivalis ribosome with Lefamulin 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-52636: 70S P. gingivalis ribosome erm-delta-porN strain consensus map 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-52637: Focus refined 50S map of 70S P. gingivalis ribosome erm-delta-porN strain 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-52638: Focus refined 30S body map of 70S P. gingivalis ribosome erm-delta-porN strain 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-52639: Focus refined 30S head map of 70S P. gingivalis ribosome erm-delta-porN strain 手法: EM (単粒子) / 解像度: 2.9 Å 52640 9i5v EMDB-52640, PDB-9i5v: 70S P. gingivalis ribosome erm-delta-porN strain 手法: EM (単粒子) / 解像度: 2.7 Å 52641 9i5x EMDB-52641, PDB-9i5x: Porphyromonas gingivalis 70S ribosome (W83 Strain) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-52767: Focus refined 50S map of 70S P. gingivalis ribosome W83 strain 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-52768: Focus refined 30S body map of 70S P. gingivalis ribosome W83 strain 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-52770: Focus refined 30S head map of 70S P. gingivalis ribosome W83 strain 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-62B: Lefamulin / 薬剤, 抗生剤YM ChemComp-K: Unknown entry ChemComp-NA: Unknown entry ChemComp-HOH*: WATER
由来	porphyromonas gingivalis (バクテリア)
キーワード	RIBOSOME / antibiotics / macrolide-resistance / macrolide-resistant / Antibiotic / Lefamulin