+検索条件
-Structure paper
タイトル | Cryo-EM structure of respiratory complex IV. |
---|---|
ジャーナル・号・ページ | IUCrJ, Vol. 6, Issue Pt 4, Page 773-780, Year 2019 |
掲載日 | 2019年7月1日 |
![]() | Thomas Bausewein / Stephan Nussberger / Werner Kühlbrandt / ![]() |
PubMed 要旨 | In fungi, the mitochondrial respiratory chain complexes (complexes I-IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane ...In fungi, the mitochondrial respiratory chain complexes (complexes I-IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane protein from in lipid nanodiscs as cytochrome oxidase (complex IV) and its structure was determined at 5.5 Å resolution. The map closely resembles the cryo-EM structure of complex IV from . Its ten subunits are conserved in and , but other transmembrane subunits are missing. The different structure of the Cox5a subunit is typical for fungal complex IV and may affect the interaction with complex III in a respiratory supercomplex. Additional density was found between the matrix domains of the Cox4 and Cox5a subunits that appears to be specific to . |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) |
解像度 | 5.5 Å |
構造データ | ![]() EMDB-4720: |
由来 |
|