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-Structure paper
Title | Cryo-EM structure of respiratory complex IV. |
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Journal, issue, pages | IUCrJ, Vol. 6, Issue Pt 4, Page 773-780, Year 2019 |
Publish date | Jul 1, 2019 |
![]() | Thomas Bausewein / Stephan Nussberger / Werner Kühlbrandt / ![]() |
PubMed Abstract | In fungi, the mitochondrial respiratory chain complexes (complexes I-IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane ...In fungi, the mitochondrial respiratory chain complexes (complexes I-IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane protein from in lipid nanodiscs as cytochrome oxidase (complex IV) and its structure was determined at 5.5 Å resolution. The map closely resembles the cryo-EM structure of complex IV from . Its ten subunits are conserved in and , but other transmembrane subunits are missing. The different structure of the Cox5a subunit is typical for fungal complex IV and may affect the interaction with complex III in a respiratory supercomplex. Additional density was found between the matrix domains of the Cox4 and Cox5a subunits that appears to be specific to . |
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Methods | EM (single particle) |
Resolution | 5.5 Å |
Structure data | ![]() EMDB-4720: |
Source |
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