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-Structure paper
タイトル | Hachiman is a genome integrity sensor. |
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ジャーナル・号・ページ | bioRxiv, Year 2024 |
掲載日 | 2024年2月29日 |
著者 | Owen T Tuck / Benjamin A Adler / Emily G Armbruster / Arushi Lahiri / Jason J Hu / Julia Zhou / Joe Pogliano / Jennifer A Doudna / |
PubMed 要旨 | Hachiman is a broad-spectrum antiphage defense system of unknown function. We show here that Hachiman comprises a heterodimeric nuclease-helicase complex, HamAB. HamA, previously a protein of unknown ...Hachiman is a broad-spectrum antiphage defense system of unknown function. We show here that Hachiman comprises a heterodimeric nuclease-helicase complex, HamAB. HamA, previously a protein of unknown function, is the effector nuclease. HamB is the sensor helicase. HamB constrains HamA activity during surveillance of intact dsDNA. When the HamAB complex detects DNA damage, HamB helicase activity liberates HamA, unleashing nuclease activity. Hachiman activation degrades all DNA in the cell, creating 'phantom' cells devoid of both phage and host DNA. We demonstrate Hachiman activation in the absence of phage by treatment with DNA-damaging agents, suggesting that Hachiman responds to aberrant DNA states. Phylogenetic similarities between the Hachiman helicase and eukaryotic enzymes suggest this bacterial immune system has been repurposed for diverse functions across all domains of life. |
リンク | bioRxiv / PubMed:38464307 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.65 - 3.19 Å |
構造データ | EMDB-43613, PDB-8vx9: EMDB-43615, PDB-8vxa: EMDB-43616, PDB-8vxc: EMDB-43643, PDB-8vxy: |
化合物 | ChemComp-ATP: |
由来 |
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キーワード | IMMUNE SYSTEM / Nuclease-helicase complex / antiphage defense system / IMMUNE SYSTEM/DNA / Helicase-DNA complex / IMMUNE SYSTEM-DNA complex |