EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 3296, Year 2024
掲載日	2024年4月17日
著者	Victor E Cruz / Christine S Weirich / Nagesh Peddada / Jan P Erzberger /
PubMed 要旨	DEAD-box ATPases play crucial roles in guiding rRNA restructuring events during the biogenesis of large (60S) ribosomal subunits, but their precise molecular functions are currently unknown. In this ...DEAD-box ATPases play crucial roles in guiding rRNA restructuring events during the biogenesis of large (60S) ribosomal subunits, but their precise molecular functions are currently unknown. In this study, we present cryo-EM reconstructions of nucleolar pre-60S intermediates that reveal an unexpected, alternate secondary structure within the nascent peptidyl-transferase-center (PTC). Our analysis of three sequential nucleolar pre-60S intermediates reveals that the DEAD-box ATPase Dbp10/DDX54 remodels this alternate base pairing and enables the formation of the rRNA junction that anchors the mature form of the universally conserved PTC A-loop. Post-catalysis, Dbp10 captures rRNA helix H61, initiating the concerted exchange of biogenesis factors during late nucleolar 60S maturation. Our findings show that Dbp10 activity is essential for the formation of the ribosome active site and reveal how this function is integrated with subsequent assembly steps to drive the biogenesis of the large ribosomal subunit.
リンク	Nat Commun / PubMed:38632236 / PubMed Central
手法	EM (単粒子)
解像度	2.47 - 3.58 Å
構造データ	43017 8v83 EMDB-43017, PDB-8v83: 60S ribosome biogenesis intermediate (Dbp10 pre-catalytic structure - Overall map) 手法: EM (単粒子) / 解像度: 2.53 Å EMDB-43018: 60S ribosome biogenesis intermediate (Dbp10 pre-catalytic structure - PTC Local map) 手法: EM (単粒子) / 解像度: 2.69 Å EMDB-43019: 60S ribosome biogenesis intermediate (Dbp10 pre-catalytic structure - Local map L1 region) 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-43020: 60S ribosome biogenesis intermediate (Dbp10 pre-catalytic structure - Local map Rrp14/Rrp15/Ssf1 region) 手法: EM (単粒子) / 解像度: 2.47 Å 43021 8v84 EMDB-43021, PDB-8v84: 60S ribosome biogenesis intermediate (Dbp10 catalytic structure - Overall map) 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-43022: 60S ribosome biogenesis intermediate (Dbp10 catalytic structure - Dbp10 Local map) 手法: EM (単粒子) / 解像度: 2.9 Å 43023 8v85 EMDB-43023, PDB-8v85: 60S ribosome biogenesis intermediate (Dbp10 catalytic structure - Low-pass filtered locally refined map) 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-43024: 60S ribosome biogenesis intermediate (Dbp10 catalytic structure - L1 local map 手法: EM (単粒子) / 解像度: 3.57 Å EMDB-43026: 60S ribosome biogenesis intermediate (Dbp10 catalytic intermediate - Rrp14/Rrp15/Ssf1 local map) 手法: EM (単粒子) / 解像度: 2.81 Å 43027 8v87 EMDB-43027, PDB-8v87: 60S ribosome biogenesis intermediate (Dbp10 post-catalytic structure - Overall map) 手法: EM (単粒子) / 解像度: 2.66 Å EMDB-43028: 60S ribosome biogenesis intermediate (Dbp10 post-catalytic structure - Dbp10 Local map) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-43029: 60S ribosome biogenesis intermediate (Dbp10 post-catalytic structure - H64 Local map) 手法: EM (単粒子) / 解像度: 2.92 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-K: Unknown entry / カリウムカチオン
由来	saccharomyces cerevisiae by4741 (パン酵母) saccharomyces cerevisiae by4741-av16 (パン酵母)
キーワード	RIBOSOME / Ribosome biogenesis intermediate DEAD-box ATPase / Ribosome/RNA / Ribosome biogenesis / intermediate DEAD-box ATPase / Ribosome-RNA complex / RNA BINDING PROTEIN / RNA Binding