EMDB-43027: 60S ribosome biogenesis intermediate (Dbp10 post-catalytic struct...

Basic information

Entry

Database: EMDB / ID: EMD-43027

• Title: 60S ribosome biogenesis intermediate (Dbp10 post-catalytic structure - Overall map)
• Map data: 60S ribosome biogenesis intermediate (Dbp10 post-catalytic structure - Overall map)

Sample

• Complex: 60S ribosome biogenesis intermediate
  • RNA: x 3 types
  • Protein or peptide: x 53 types
• Ligand: x 5 types

• Keywords: Ribosome biogenesis / intermediate DEAD-box ATPase / Ribosome-RNA complex

Function / homology

Function:

25S rRNA (cytosine2870-C5)-methyltransferase / 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase / Noc1p-Noc2p complex / snoRNA release from pre-rRNA / rRNA (guanosine-2'-O-ribose)-methyltransferase activity / Noc2p-Noc3p complex / nuclear division / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA (uridine-2'-O-ribose)-methyltransferase activity / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA (guanine) methyltransferase activity / rRNA (cytosine-C5-)-methyltransferase activity / RNA strand annealing activity / PeBoW complex / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / intracellular mRNA localization / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / RNA folding chaperone / nuclear pre-replicative complex / positive regulation of ATP-dependent activity / rRNA primary transcript binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ATP-dependent activity, acting on RNA / rRNA base methylation / rRNA methylation / pre-mRNA 5'-splice site binding / Ribosome Quality Control (RQC) complex extracts and degrades nascent peptide / maturation of 5.8S rRNA / cytosolic large ribosomal subunit assembly / Major pathway of rRNA processing in the nucleolus and cytosol / PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / proteasome binding / ATPase activator activity / Formation of a pool of free 40S subunits / ribosomal large subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / negative regulation of mRNA splicing, via spliceosome / ribosomal large subunit export from nucleus / translational elongation / DNA replication initiation / protein folding chaperone complex / nuclear-transcribed mRNA catabolic process / ribonucleoprotein complex binding / 90S preribosome / ribosomal subunit export from nucleus / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / maturation of LSU-rRNA / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation initiation factor activity / nuclear periphery / proteasome complex / macroautophagy / cytosolic ribosome assembly / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / assembly of large subunit precursor of preribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / molecular condensate scaffold activity / protein catabolic process / maintenance of translational fidelity / ribosome biogenesis / rRNA processing / nuclear envelope / ATPase binding / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / RNA helicase activity / protein-macromolecule adaptor activity / rRNA binding / negative regulation of translation / RNA helicase / structural constituent of ribosome / ribosome / translation / mRNA binding / chromatin binding / GTPase activity / nucleolus / GTP binding / ATP hydrolysis activity / mitochondrion / DNA binding / RNA binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytosol

Domain/homology:

DBP10, C-terminal / DDX54/DBP10 family / DBP10CT (NUC160) domain / DBP10CT (NUC160) domain / 60S ribosomal subunit assembly/export protein Loc1 / : / LOC1 helical domain / Nucleolar complex protein 2 / Nucleolar complex-associated protein 3, N-terminal / Nucleolar complex-associated protein 3 / Noc2p family / Nucleolar complex-associated protein / WD repeat WDR12/Ytm1 / Ribosome biogenesis factor, NIP7 / RNA (C5-cytosine) methyltransferase, NOP2 / : / UPF0113, pre-PUA domain / UPF0113 PUA domain / NIP7/UPF0113, pre-PUA domain / UPF0113, PUA domain / Nop2p / Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site / NOL1/NOP2/sun family signature. / Ribosome biogenesis protein 15, RNA recognition motif / Ribosomal RNA methyltransferase, SPB1-like, C-terminal / Ribosomal RNA methyltransferase Spb1, domain of unknown function DUF3381 / AdoMet-dependent rRNA methyltransferase SPB1-like / Spb1 C-terminal domain / Ribosomal RNA methyltransferase Spb1, DUF3381 / : / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / Ribosomal RNA large subunit methyltransferase E / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein BRX1 / DDX18/Has1, DEAD-box helicase domain / Domain of unknown function DUF2423 / YBL028C ribosome biogenesis factor, N-terminal domain / Domain of unknown function DUF4217 / ATP-dependent rRNA helicase SPB4-like, C-terminal extension / DUF4217 / BOP1, N-terminal domain / WD repeat BOP1/Erb1 / BOP1NT (NUC169) domain / BOP1NT (NUC169) domain / NLE / NLE (NUC135) domain / Guanine nucleotide-binding protein-like 3, N-terminal domain / GNL3L/Grn1 putative GTPase / Putative RNA-binding Domain in PseudoUridine synthase and Archaeosine transglycosylase / PUA domain / SAM-dependent methyltransferase RsmB/NOP2-type / RNA (C5-cytosine) methyltransferase / : / 16S rRNA methyltransferase RsmB/F / SAM-dependent MTase RsmB/NOP-type domain profile. / CCAAT-binding factor / CBF/Mak21 family / PUA domain superfamily / PUA domain profile. / Pescadillo / Pescadillo N-terminus / : / Ribosomal biogenesis NSA2 family / GTP-binding protein, orthogonal bundle domain superfamily / Ribosome assembly factor Mrt4 / BRCT domain / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Brix domain / Brix domain / Brix domain profile. / Brix / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / 50S ribosome-binding GTPase / DEAD-box RNA helicase Q motif profile. / GTP binding domain / 50S ribosomal protein L10, insertion domain superfamily / PUA-like superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / breast cancer carboxy-terminal domain / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1

Component:

Ribosome biogenesis protein YTM1 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase / Large ribosomal subunit protein uL13A / Ribosome assembly factor MRT4 / rRNA-processing protein EBP2 / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / UPF0642 protein YBL028C / Proteasome-interacting protein CIC1 / Nucleolar complex protein 2 / Nucleolar protein 16 / Nuclear GTP-binding protein NUG1 / Ribosome biogenesis protein NSA2 / Ribosome biogenesis protein RLP7 / 25S rRNA (cytosine(2870)-C(5))-methyltransferase / 60S ribosomal subunit assembly/export protein LOC1 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Pescadillo homolog / Ribosome biogenesis protein 15 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL6A / Nucleolar GTP-binding protein 1 / ATP-dependent RNA helicase HAS1 / Ribosome biogenesis protein ERB1 / Nucleolar complex-associated protein 3 / Ribosome biogenesis protein RLP24 / Ribosome biogenesis protein BRX1 / 60S ribosome subunit biogenesis protein NIP7 / ATP-dependent RNA helicase DBP10 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein eL13A

• Biological species: Saccharomyces cerevisiae BY4741 (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 2.66 Å
• Authors: Cruz VE / Weirich CS / Peddada N / Erzberger JP

Funding support

United States, 3 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	1R01GM135617-01	United States
Robert A. Welch Foundation	I-1897	United States
Cancer Prevention and Research Institute of Texas (CPRIT)	RR150074	United States

• Citation: Journal: Nat Commun / Year: 2024; Title: The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis.; Authors: Victor E Cruz / Christine S Weirich / Nagesh Peddada / Jan P Erzberger / ; Abstract: DEAD-box ATPases play crucial roles in guiding rRNA restructuring events during the biogenesis of large (60S) ribosomal subunits, but their precise molecular functions are currently unknown. In this study, we present cryo-EM reconstructions of nucleolar pre-60S intermediates that reveal an unexpected, alternate secondary structure within the nascent peptidyl-transferase-center (PTC). Our analysis of three sequential nucleolar pre-60S intermediates reveals that the DEAD-box ATPase Dbp10/DDX54 remodels this alternate base pairing and enables the formation of the rRNA junction that anchors the mature form of the universally conserved PTC A-loop. Post-catalysis, Dbp10 captures rRNA helix H61, initiating the concerted exchange of biogenesis factors during late nucleolar 60S maturation. Our findings show that Dbp10 activity is essential for the formation of the ribosome active site and reveal how this function is integrated with subsequent assembly steps to drive the biogenesis of the large ribosomal subunit.

History

Deposition	Dec 4, 2023	-
Header (metadata) release	May 1, 2024	-
Map release	May 1, 2024	-
Update	May 1, 2024	-
Current status	May 1, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_43027.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: 60S ribosome biogenesis intermediate (Dbp10 post-catalytic structure - Overall map)
• Voxel size: X=Y=Z: 1.08 Å

Density

Contour Level	By AUTHOR: 0.04
Minimum - Maximum	-0.19055663 - 0.34836707
Average (Standard dev.)	0.00021373158 (±0.01191797)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 388.80002 Å α=β=γ: 90.0 °

Supplemental data

Half map: Half Map A

• File: emd_43027_half_map_1.map
• Annotation: Half Map A

Half map: Half Map B

• File: emd_43027_half_map_2.map
• Annotation: Half Map B

Sample components

Entire : 60S ribosome biogenesis intermediate

• Entire: Name: 60S ribosome biogenesis intermediate

Components

• Complex: 60S ribosome biogenesis intermediate
  • RNA: 25S ribosomal RNA
  • RNA: 5.8S ribosomal RNA
  • Protein or peptide: ATP-dependent RNA helicase DBP10
  • RNA: ITS2 RNA
  • Protein or peptide: 60S ribosomal subunit assembly/export protein LOC1
  • Protein or peptide: Nucleolar complex protein 2
  • Protein or peptide: Ribosome biogenesis protein BRX1
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L4-A
  • Protein or peptide: ATP-dependent RNA helicase HAS1
  • Protein or peptide: 60S ribosomal protein L6-A
  • Protein or peptide: 60S ribosomal protein L7-A
  • Protein or peptide: Large ribosomal subunit protein eL8A
  • Protein or peptide: Large ribosomal subunit protein uL6A
  • Protein or peptide: Nucleolar complex-associated protein 3
  • Protein or peptide: rRNA-processing protein EBP2
  • Protein or peptide: Proteasome-interacting protein CIC1
  • Protein or peptide: 60S ribosomal protein L13-A
  • Protein or peptide: 60S ribosomal protein L14-A
  • Protein or peptide: Large ribosomal subunit protein eL15A
  • Protein or peptide: Large ribosomal subunit protein uL13A
  • Protein or peptide: 60S ribosomal protein L17-A
  • Protein or peptide: 60S ribosomal protein L18-A
  • Protein or peptide: 60S ribosomal protein L19-A
  • Protein or peptide: Large ribosomal subunit protein eL20A
  • Protein or peptide: 60S ribosomal protein L22-A
  • Protein or peptide: 60S ribosomal protein L23-A
  • Protein or peptide: Ribosome assembly factor MRT4
  • Protein or peptide: 60S ribosomal protein L25
  • Protein or peptide: 60S ribosomal protein L26-A
  • Protein or peptide: 60S ribosomal protein L27-A
  • Protein or peptide: Large ribosomal subunit protein uL1A
  • Protein or peptide: Nucleolar GTP-binding protein 1
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31-A
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L33-A
  • Protein or peptide: 60S ribosomal protein L34-A
  • Protein or peptide: 60S ribosomal protein L35-A
  • Protein or peptide: 60S ribosomal protein L36-A
  • Protein or peptide: 60S ribosomal protein L37-A
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: 60S ribosome subunit biogenesis protein NIP7
  • Protein or peptide: Ribosome biogenesis protein ERB1
  • Protein or peptide: Pescadillo homolog
  • Protein or peptide: Ribosome biogenesis protein 15
  • Protein or peptide: YTM1 isoform 1
  • Protein or peptide: 25S rRNA (cytosine(2870)-C(5))-methyltransferase
  • Protein or peptide: Ribosome biogenesis protein NSA2
  • Protein or peptide: Nuclear GTP-binding protein NUG1
  • Protein or peptide: Ribosome biogenesis protein RLP7
  • Protein or peptide: Ribosome biogenesis protein RLP24
  • Protein or peptide: Nucleolar protein 16
  • Protein or peptide: 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • Protein or peptide: UPF0642 protein YBL028C
• Ligand: ADENOSINE-5'-DIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: POTASSIUM ION
• Ligand: ZINC ION

Supramolecule #1: 60S ribosome biogenesis intermediate

• Supramolecule: Name: 60S ribosome biogenesis intermediate / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)

Macromolecule #1: 25S ribosomal RNA

• Macromolecule: Name: 25S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 1.0974938749999998 MDa

Sequence

String:

GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG GCCAGCAUCA GUUUUGGUGG CAGGAUAAAU CCAUAGGAAU GUAGCUUGCC UCGGUAAGUA UUAUAG CCU GUGGGAAUAC UGCCAGCUGG GACUGAGGAC UGCGACGUAA GUCAAGGAUG CUGGCAUAAU GGUUAUAUGC CGCCCGU CU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUA G GUUGGGGCCU CGCAAGAGGU GCACAAUCGA CCGAUCCUGA UGUCUUCGGA UGGAUUUGAG UAAGAGCAUA GCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAUA GGGUGAAGCC AGAGGAAACU CUGGUGGAGG CUCGUAGCGG UUCUGACGUG CAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCUGCC GAAGUUUCCC U CAGGAUAG CAGAAGCUCG UAUCAGUUUU AUGAGGUAAA GCGAAUGAUU AGAGGUUCCG GGGUCGAAAU GACCUUGACC UA UUCUCAA ACUUUAAAUA UGUAAGAAGU CCUUGUUACU UAAUUGAACG UGGACAUUUG AAUGAAGAGC UUUUAGUGGG CCA UUUUUG GUAAGCAGAA CUGGCGAUGC GGGAUGAACC GAACGUAGAG UUAAGGUGCC GGAAUACACG CUCAUCAGAC ACCA CAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACC GGCC GAAUGAACUA GCCCUGAAAA UGGAUGGCGC UCAAGCGUGU UACCUAUACU CUACCGUCAG GGUUGAUAUG AUGCCC UGA CGAGUAGGCA GGCGUGGAGG UCAGUGACGA AGCCUAGACC GUAAGGUCGG GUCGAACGGC CUCUAGUGCA GAUCUUG GU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGG G UUAGUCGAUC CUAAGAGAUG GGGAAGCUCC GUUUCAAAGG CCUGAUUUUA UGCAGGCCAC CAUCGAAAGG GAAUCCGGU UAAGAUUCCG GAACCUGGAU AUGGAUUCUU CACGGUAACG UAACUGAAUG UGGAGACGUC GGCGCGAGCC CUGGGAGGAG UUAUCUUUU CUUCUUAACA GCUUAUCACC CCGGAAUUGG UUUAUCCGGA GAUGGGGUCU UAUGGCUGGA AGAGGCCAGC A CCUUUGCU GGCUCCGGUG CGCUUGUGAC GGCCCGUGAA AAUCCACAGG AAGGAAUAGU UUUCAUGCCA GGUCGUACUG AU AACCGCA GCAGGUCUCC AAGGUGAACA GCCUCUAGUU GAUAGAAUAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGU AACUUC GGGAUAAGGA UUGGCUCUAA GGGUCGGGUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUAAC GAUCAACUUA GAACUGGUAC GGACAAGGGG AAUCUGACUG UCUAAUUAAA ACAUAGCAUU GCGAUG GUC AGAAAGUGAU GUUGACGCAA UGUGAUUUCU GCCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAACCAA GCGCGGG UA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUA A CGAGAUUCCC ACUGUCCCUA UCUACUAUCU AGCGAAACCA CAGCCAAGGG AACGGGCUUG GCAGAAUCAG CGGGGAAAG AAGACCCUGU UGAGCUUGAC UCUAGUUUGA CAUUGUGAAG AGACAUAGAG GGUGUAGAAU AAGUGGGAGC UUCGGCGCCA GUGAAAUAC CACUACCUUU AUAGUUUCUU UACUUAUUCA AUGAAGCGGA GCUGGAAUUC AUUUUCCACG UUCUAGCAUU C AAGGUCCC AUUCGGGGCU GAUCCGGGUU GAAGACAUUG UCAGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC GA UAACGCA GAUGUCCUAA GGGGGGCUCA UGGAGAACAG AAAUCUCCAG UAGAACAAAA GGGUAAAAGC CCCCUUGAUU UUG AUUUUC AGUGUGAAUA CAAACCAUGA AAGUGUGGCC UAUCGAUCCU UUAGUCCCUC GGAAUUUGAG GCUAGAGGUG CCAG AAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCU CUUC CUAUCAUACC GAAGCAGAAU UCGGUAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACC GUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AAUGUUACCG CAAUAGUAAU UGAACUUAGU ACGAGAGGAA CAGUUCA UU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUC U AAGUCAGAAU CCAUGCUAGA ACGCGGUGAU UUCUUUGCUC CACACAAUAU AGAUGGAUAC GAAUAAGGCG UCCUUGUGG CGUCGCUGAA CCAUAGCAGG CUAGCAACGG UGCACUUGGC GGAAAGGCCU UGGGUGCUUG CUGGCGAAUU GCAAUGUCAU UUUGCGUGG GGAUAAAUCA UUUGUAUACG ACUUAGAUGU ACAACGGGGU AUUGUAAGCA GUAGAGUAGC CUUGUUGUUA C GAUCUGCU GAGAUUAAGC CUUUGUUGUC UGAUUUGU

GENBANK: GENBANK: CP093744.1

Macromolecule #2: 5.8S ribosomal RNA

• Macromolecule: Name: 5.8S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MN648704.1

Macromolecule #4: ITS2 RNA

• Macromolecule: Name: ITS2 RNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 74.308391 KDa

Sequence

String:

CCUUCUCAAA CAUUCUGUUU GGUAGUGAGU GAUACUCUUU GGAGUUAACU UGAAAUUGCU GGCCUUUUCA UUGGAUGUUU UUUUUCCAA AGAGAGGUUU CUCUGCGUGC UUGAGGUAUA AUGCAAGUAC GGUCGUUUUA GGUUUUACCA ACUGCGGCUA A UCUUUUUU UAUACUGAGC GUAUUGGAAC GUUAUCGAUA AGAAGAGAGC GUCUAGGCGA ACAAUGUUCU UAAA

Macromolecule #3: ATP-dependent RNA helicase DBP10

• Macromolecule: Name: ATP-dependent RNA helicase DBP10 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 113.126469 KDa

Sequence

String:

MAGVQKRKRD LEDQDDNGSE EDDIAFDIAN EIALNDSESD ANDSDSEVEA DYGPNDVQDV IEYSSDEEEG VNNKKKAENK DIKKKKNSK KEIAAFPMLE MSDDENNASG KTQTGDDEDD VNEYFSTNNL EKTKHKKGSF PSFGLSKIVL NNIKRKGFRQ P TPIQRKTI PLILQSRDIV GMARTGSGKT AAFILPMVEK LKSHSGKIGA RAVILSPSRE LAMQTFNVFK DFARGTELRS VL LTGGDSL EEQFGMMMTN PDVIIATPGR FLHLKVEMNL DLKSVEYVVF DEADRLFEMG FQEQLNELLA SLPTTRQTLL FSA TLPNSL VDFVKAGLVN PVLVRLDAET KVSENLEMLF LSSKNADREA NLLYILQEII KIPLATSEQL QKLQNSNNEA DSDS DDEND RQKKRRNFKK EKFRKQKMPA ANELPSEKAT ILFVPTRHHV EYISQLLRDC GYLISYIYGT LDQHARKRQL YNFRA GLTS ILVVTDVAAR GVDIPMLANV INYTLPGSSK IFVHRVGRTA RAGNKGWAYS IVAENELPYL LDLELFLGKK ILLTPM YDS LVDVMKKRWI DEGKPEYQFQ PPKLSYTKRL VLGSCPRLDV EGLGDLYKNL MSSNFDLQLA KKTAMKAEKL YYRTRTS AS PESLKRSKEI ISSGWDAQNA FFGKNEEKEK LDFLAKLQNR RNKETVFEFT RNPDDEMAVF MKRRRKQLAP IQRKATER R ELLEKERMAG LSHSIEDEIL KGDDGETGYT VSEDALKEFE DADQLLEAQE NENKKKKKPK SFKDPTFFLS HYAPAGDIQ DKQLQITNGF ANDAAQAAYD LNSDDKVQVH KQTATVKWDK KRKKYVNTQG IDNKKYIIGE SGQKIAASFR SGRFDDWSKA RNLKPLKVG SRETSIPSNL LEDPSQGPAA NGRTVRGKFK HKQMKAPKMP DKHRDNYYSQ KKKVEKALQS GISVKGYNNA P GLRSELKS TEQIRKDRII AEKKRAKNAR PSKKRKF

UniProtKB: ATP-dependent RNA helicase DBP10

Macromolecule #5: 60S ribosomal subunit assembly/export protein LOC1

• Macromolecule: Name: 60S ribosomal subunit assembly/export protein LOC1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 23.652645 KDa

Sequence

String:

MAPKKPSKRQ NLRREVAPEV FQDSQARNQL ANVPHLTEKS AQRKPSKTKV KKEQSLARLY GAKKDKKGKY SEKDLNIPTL NRAIVPGVK IRRGKKGKKF IADNDTLTLN RLITTIGDKY DDIAESKLEK ARRLEEIREL KRKEIERKEA LKQDKLEEKK D EIKKKSSV ARTIRRKNKR DMLKSEAKAS ESKTEGRKVK KVSFAQ

UniProtKB: 60S ribosomal subunit assembly/export protein LOC1

Macromolecule #6: Nucleolar complex protein 2

• Macromolecule: Name: Nucleolar complex protein 2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 81.719289 KDa

Sequence

String:

MGKVSKSTKK FQSKHLKHTL DQRRKEKIQK KRIQGRRGNK TDQEKADAAG TREQQQLKKS AKEEVFKDMS VETFFEKGIE IPKENKKLK KKTTKEQSDE DSSSSEEEED MGQSMAKLAE KDPEFYKYLE ENDKDLLDFA GTNPLDGIDS QDEGEDAERN S NIEEKSEQ MELEKEKIEL SLKLVRKWKK QLHDSPSLKL LRNIISAFKV AVNLNKEENI EDYKYAITDE KAFHELMFMV LK DVPQAIQ KMAPYKIVKG ARTLPNGGNV SRVSSIVKSH AGSLLILLND ITNTETAALV LHSVNELMPY LLSYRRILKE LIK SIVGVW STTRELETQI ASFAFLINTT KEFKKSMLET TLKTTYSTFI KSCRKTNMRS MPLINFQKNS AAELFGIDEV LGYQ VGFEY IRQLAIHLRN TMNATTKKSS KINSAEAYKI VYNWQFCHSL DFWSRVLSFA CQPEKENGSE SPLRQLIYPL VQVTL GVIR LIPTPQFFPL RFYLIKSLIR LSQNSGVFIP IYPLLSEILT STAFTKAPKK SPNLAAFDFE HNIKCTQAYL NTKIYQ EGL SEQFVDLLGD YFALYCKNIA FPELVTPVII SLRRYIKTST NVKLNKRLST VVEKLNQNST FIQEKRSDVE FGPTNKS EV SRFLNDVAWN KTPLGSYVAV QREVKEEKAR LMRESMEEQD KERETEEAKL LNSLESDDDN EDVEMSDA

UniProtKB: Nucleolar complex protein 2

Macromolecule #7: Ribosome biogenesis protein BRX1

• Macromolecule: Name: Ribosome biogenesis protein BRX1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 33.585414 KDa

Sequence

String:

MSSIYKALAG KSKDNKSEKK QGNVKQFMNK QRTLLISSRG VNYRHRHLIQ DLSGLLPHSR KEPKLDTKKD LQQLNEIAEL YNCNNVLFF EARKHQDLYL WLSKPPNGPT IKFYIQNLHT MDELNFTGNC LKGSRPVLSF DQRFESSPHY QLIKELLVHN F GVPPNARK SKPFIDHVMS FSIVDDKIWV RTYEISHSTK NKEEYEDGEE DISLVEIGPR FVMTVILILE GSFGGPKIYE NK QYVSPNV VRAQIKQQAA EEAKSRAEAA VERKIKRREN VLAADPLSND ALFK

UniProtKB: Ribosome biogenesis protein BRX1

Macromolecule #8: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 43.850793 KDa

Sequence

String:

MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #9: 60S ribosomal protein L4-A

• Macromolecule: Name: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 39.159125 KDa

Sequence

String:

MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

UniProtKB: Large ribosomal subunit protein uL4A

Macromolecule #10: ATP-dependent RNA helicase HAS1

• Macromolecule: Name: ATP-dependent RNA helicase HAS1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 56.798348 KDa

Sequence

String:

MATPSNKRSR DSESTEEPVV DEKSTSKQNN AAPEGEQTTC VEKFEELKLS QPTLKAIEKM GFTTMTSVQA RTIPPLLAGR DVLGAAKTG SGKTLAFLIP AIELLHSLKF KPRNGTGIIV ITPTRELALQ IFGVARELME FHSQTFGIVI GGANRRQEAE K LMKGVNML IATPGRLLDH LQNTKGFVFK NLKALIIDEA DRILEIGFED EMRQIIKILP NEDRQSMLFS ATQTTKVEDL AR ISLRPGP LFINVVPETD NSTADGLEQG YVVCDSDKRF LLLFSFLKRN QKKKIIVFLS SCNSVKYYAE LLNYIDLPVL ELH GKQKQQ KRTNTFFEFC NAERGILICT DVAARGLDIP AVDWIIQFDP PDDPRDYIHR VGRTARGTKG KGKSLMFLTP NELG FLRYL KASKVPLNEY EFPENKIANV QSQLEKLIKS NYYLHQTAKD GYRSYLQAYA SHSLKTVYQI DKLDLAKVAK SYGFP VPPK VNITIGASGK TPNTKRRKTH K

UniProtKB: ATP-dependent RNA helicase HAS1

Macromolecule #11: 60S ribosomal protein L6-A

• Macromolecule: Name: 60S ribosomal protein L6-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 20.000564 KDa

Sequence

String:

MSAQKAPKWY PSEDVAALKK TRKAARPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLISGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQNKE IKAERVEDQK VVDKALIAEI KKTPLLKQYL S ASFSLKNG DKPHMLKF

UniProtKB: Large ribosomal subunit protein eL6A

Macromolecule #12: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 27.686281 KDa

Sequence

String:

MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #13: Large ribosomal subunit protein eL8A

• Macromolecule: Name: Large ribosomal subunit protein eL8A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 28.17582 KDa

Sequence

String:

MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #14: Large ribosomal subunit protein uL6A

• Macromolecule: Name: Large ribosomal subunit protein uL6A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

Macromolecule #15: Nucleolar complex-associated protein 3

• Macromolecule: Name: Nucleolar complex-associated protein 3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 75.689008 KDa

Sequence

String:

MAKRNRSQFR IQERTAKKRK HEDSLLEGNV FQNAPEDMDE NTIYSAKGSS WDEEEQDYEM VPRKNRSDTS NLVEGLPIKV NGKVERKLH KAQEKPKDDD EEDEDSNDSS EDDEGPNEEQ EAEAKEDEPD TEEKILQLKE DIADLVTKVM EEPEENTAAL G RLCKMVES KNPNTCKFSM LALVPVFKSI IPGYRIRPLT ETEKKEKVSK EVSKLRNFEQ ALVYNYKNYV GRLQSLSKTP SN AAPIQVS LGILATQAAK ELISTASHFN FRTDIFTLLL RRICKPRIST DPTSIQIIQT FETLLNEDEE GSISFEILRI FNK ILKTRN FNIEESVLNM LLSLDVLHDY DPNTKLKGNV SAPKLKKKDR VHLSKKQRKA RKEMQQIEEE MRNAEQAVSA EERE RNQSE ILKIVFTIYL NILKNNAKTL IGSVLEGLTK FGNMANFDLL GDFLEVMKEL ISDTEFDNLS SAEVRKALLC IVSAF SLIS NTQYMKVNVD LSKFVDGLYA LLPYICLDAD IELSYRSLRL ADPLNNEIIK PSVNVSTKAE LLLKALDHVF FRSKSG TKE RATAFTKRLY MCISHTPEKT SIAILKFIDK LMNRYPEISG LYSSEDRIGN GHFIMEADNP SRSNPEAATL WDNALLE KH YCPVVTKGLR SLSSRSKECS K

UniProtKB: Nucleolar complex-associated protein 3

Macromolecule #16: rRNA-processing protein EBP2

• Macromolecule: Name: rRNA-processing protein EBP2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 49.842613 KDa

Sequence

String:

MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK LEKKEKKADV KKEVAADTEE YQSQALSKKE KRKLKKELK KMQEQDATEA QKHMSGDEDE SGDDREEEEE EEEEEEGRLD LEKLAKSDSE SEDDSESEND SEEDEDVVAK E ESEEKEEQ EEEQDVPLSD VEFDSDADVV PHHKLTVNNT KAMKHALERV QLPWKKHSFQ EHQSVTSETN TDEHIKDIYD DT ERELAFY KQSLDAVLVA RDELKRLKVP FKRPLDYFAE MVKSDEHMDK IKGKLIEEAS DKKAREEARR QRQLKKFGKQ VQN ATLQKR QLEKRETLEK IKSLKNKRKH NEIDHSEFNV GVEEEVEGKR FDRGRPNGKR AAKNAKYGQG GMKRFKRKND ATSS ADVSG FSSRKMKGKT NRPGKSRRAR RF

UniProtKB: rRNA-processing protein EBP2

Macromolecule #17: Proteasome-interacting protein CIC1

• Macromolecule: Name: Proteasome-interacting protein CIC1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 42.596691 KDa

Sequence

String:

MAKKSNSKKS TPVSTPSKEK KKVIEKKSST AIPRERVIKA VNELIKFTSK PQDENNEEGN NGKKNLLEDD EEELKKDLQL IVVNNKSFT GTSKSFKLKL LNVKHSFYKP WKEASATAVK DFKVLLILKD SDIKKVSEDD LFDQLDSEGI KVDEIICGKD L KTVYKAYE ARNAFISQFS LILADDSIVT SLPKLMGGKA YNKVETTPIS IRTHANKEFS LTTLTNNIKK VYMNQLPVKL PR GTTLNVH LGNLEWLRPE EFVDNVELIS EQLIKAYQIR SIFIKTNRSP VLPLYYNQDV LDELEAKKDK IEETHEDDMV TID GVQVHL STFNKGLMEI ANPSELGSIF SKQINNAKKR SSSELEKESS ESEAVKKAKS

UniProtKB: Proteasome-interacting protein CIC1

Macromolecule #18: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 22.604164 KDa

Sequence

String:

MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

UniProtKB: Large ribosomal subunit protein eL13A

Macromolecule #19: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 15.195066 KDa

Sequence

String:

MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #20: Large ribosomal subunit protein eL15A

• Macromolecule: Name: Large ribosomal subunit protein eL15A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 24.482357 KDa

Sequence

String:

MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

UniProtKB: Large ribosomal subunit protein eL15A

Macromolecule #21: Large ribosomal subunit protein uL13A

• Macromolecule: Name: Large ribosomal subunit protein uL13A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 22.247227 KDa

Sequence

String:

MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #22: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 20.589518 KDa

Sequence

String:

MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #23: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 20.609252 KDa

Sequence

String:

MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

Macromolecule #24: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 21.762316 KDa

Sequence

String:

MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ARDRRAQRVA EKRDALLKED A

UniProtKB: Large ribosomal subunit protein eL19A

Macromolecule #25: Large ribosomal subunit protein eL20A

• Macromolecule: Name: Large ribosomal subunit protein eL20A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 20.478852 KDa

Sequence

String:

MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #26: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 13.711359 KDa

Sequence

String:

MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYRLAFYQ VTPEEDEEED EE

UniProtKB: Large ribosomal subunit protein eL22A

Macromolecule #27: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 14.49395 KDa

Sequence

String:

MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QAKSWRRRD GVFLYFEDNA GVIANPKGEM KGSAITGPVG KECADLWPRV ASNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

Macromolecule #28: Ribosome assembly factor MRT4

• Macromolecule: Name: Ribosome assembly factor MRT4 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 27.098012 KDa

Sequence

String:

MPRSKRSKLV TLAQTDKKGR ENKERIFDEV REALDTYRYV WVLHLDDVRT PVLQEIRTSW AGSKLIMGKR KVLQKALGEK REEEYKENL YQLSKLCSGV TGLLFTDEDV NTVKEYFKSY VRSDYSRPNT KAPLTFTIPE GIVYSRGGQI PAEEDVPMIH S LEPTMRNK FEIPTKIKAG KITIDSPYLV CTEGEKLDVR QALILKQFGI AASEFKVKVS AYYDNDSSTV ESTNINME

UniProtKB: Ribosome assembly factor MRT4

Macromolecule #29: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 15.787612 KDa

Sequence

String:

MAPSAKATAA KKAVVKGTNG KKALKVRTSA TFRLPKTLKL ARAPKYASKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN ILVFQVSMK ANKYQIKKAV KELYEVDVLK VNTLVRPNGT KKAYVRLTAD YDALDIANRI GYI

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #30: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 14.265784 KDa

Sequence

String:

MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #31: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 15.56836 KDa

Sequence

String:

MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDV EAFKSVVSTE TFEQPSQREE AKKVVKKAFE ERHQAGKNQW FFSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #32: Large ribosomal subunit protein uL1A

• Macromolecule: Name: Large ribosomal subunit protein uL1A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 24.524799 KDa

Sequence

String:

MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDD LKKLNKNKKL IKKLSKKYNA FIASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYGKVT DVRSTIKFQL K KVLCLAVA VGNVEMEEDV LVNQILMSVN FFVSLLKKNW QNVGSLVVKS SMGPAFRLY

UniProtKB: Large ribosomal subunit protein uL1A

Macromolecule #33: Nucleolar GTP-binding protein 1

• Macromolecule: Name: Nucleolar GTP-binding protein 1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 74.531227 KDa

Sequence

String:

MQLSWKDIPT VAPANDLLDI VLNRTQRKTP TVIRPGFKIT RIRAFYMRKV KYTGEGFVEK FEDILKGFPN INDVHPFHRD LMDTLYEKN HYKISLAAIS RAKSLVEQVA RDYVRLLKFG QSLFQCKQLK RAALGRMATI VKKLRDPLAY LEQVRQHIGR L PSIDPNTR TLLICGYPNV GKSSFLRCIT KSDVDVQPYA FTTKSLYVGH FDYKYLRFQA IDTPGILDRP TEEMNNIEMQ SI YAIAHLR SCVLYFMDLS EQCGFTIEAQ VKLFHSIKPL FANKSVMVVI NKTDIIRPED LDEERAQLLE SVKEVPGVEI MTS SCQLEE NVMEVRNKAC EKLLASRIEN KLKSQSRINN VLNKIHVAQP QARDDVKRTP FIPESVKNLK KYDPEDPNRR KLAR DIEAE NGGAGVFNVN LKDKYLLEDD EWKNDIMPEI LDGKNVYDFL DPEIAAKLQA LEEEEEKLEN EGFYNSDDEE EIYDG FEAS EVDDIKEKAA WIRNRQKTMI AEARNRKSLK NKAIMPRSKL TKSFGKMEEH MSTLGHDMSA LQDKQNRAAR KNRYVE RGS DVVFGDQDAL TASTENGVKL RQTDRLLDGV ADGSMRSKAD RMAKMERRER NRHAKQGESD RHNAVSLSKH LFSGKRG VG KTDFR

UniProtKB: Nucleolar GTP-binding protein 1

Macromolecule #34: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 11.430364 KDa

Sequence

String:

MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VSILEAGDSD ILTTLA

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #35: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 12.980158 KDa

Sequence

String:

MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW KRGVKGVEYR LRLRISRKRN EEEDAKNPL FSYVEPVLVA SAKGLQTVVV EEDA

UniProtKB: Large ribosomal subunit protein eL31A

Macromolecule #36: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 14.809441 KDa

Sequence

String:

MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #37: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 12.17713 KDa

Sequence

String:

MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #38: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 13.673196 KDa

Sequence

String:

MAQRVTFRRR NPYNTRSNKI KVVKTPGGIL RAQHVKKLAT RPKCGDCGSA LQGISTLRPR QYATVSKTHK TVSRAYGGSR CANCVKERI IRAFLIEEQK IVKKVVKEQT EAAKKSEKKA KK

UniProtKB: Large ribosomal subunit protein eL34A

Macromolecule #39: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 13.94264 KDa

Sequence

String:

MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #40: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 11.151259 KDa

Sequence

String:

MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #41: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 9.877395 KDa

Sequence

String:

MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

UniProtKB: Large ribosomal subunit protein eL37A

Macromolecule #42: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 8.845561 KDa

Sequence

String:

MAREITDIKQ FLELTRRADV KTATVKINKK LNKAGKPFRQ TKFKVRGSSS LYTLVINDAG KAKKLIQSLP PTLKVNRL

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #43: 60S ribosome subunit biogenesis protein NIP7

• Macromolecule: Name: 60S ribosome subunit biogenesis protein NIP7 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 20.411699 KDa

Sequence

String:

MRQLTEEETK VVFEKLAGYI GRNISFLVDN KELPHVFRLQ KDRVYYVPDH VAKLATSVAR PNLMSLGICL GKFTKTGKFR LHITSLTVL AKHAKYKIWI KPNGEMPFLY GNHVLKAHVG KMSDDIPEHA GVIVFAMNDV PLGFGVSAKS TSESRNMQPT G IVAFRQAD IGEYLRDEDT LFT

UniProtKB: 60S ribosome subunit biogenesis protein NIP7

Macromolecule #44: Ribosome biogenesis protein ERB1

• Macromolecule: Name: Ribosome biogenesis protein ERB1 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 91.830609 KDa

Sequence

String:

MMAKNNKTTE AKMSKKRAAS EESDVEEDED KLLSVDGLID AEASESDEDD DEYESAVEEK ESSSDKEAQD DSDDDSDAEL NKLLAEEEG DGEEDYDSSE FSDDTTSLTD RLSGVKLQTI VDPNIYSKYA DGSDRIIKPE INPVYDSDDS DAETQNTIGN I PLSAYDEM PHIGYDINGK RIMRPAKGSA LDQLLDSIEL PEGWTGLLDK NSGSSLNLTK EELELISKIQ RNEQTDDSIN PY EPLIDWF TRHEEVMPLT AVPEPKRRFV PSKNEAKRVM KIVRAIREGR IIPPKKLKEM KEKEKIENYQ YDLWGDSTET NDH VMHLRA PKLPPPTNEE SYNPPEEYLL SPEEKEAWEN TEYSERERNF IPQKYSALRK VPGYGESIRE RFERSLDLYL APRV RKNKL NIDPNSLIPE LPSPKDLRPF PIRCSTIYAG HKGKVRTLSI DPSGLWLATG SDDGTVRVWE ILTGREVYRT TLIDD EENP DYHIECIEWN PDANNGILAV AVGENIHLIV PPIFGYDIEN NGKTKIEDGF GYDTFGTVKK SNLEVNENGD GDEDGE NES AKNAVKKQVA QWNKPSQKQL EKDICITISC KKTVKKLSWH RKGDYFVTVQ PDSGNTSVLI HQVSKHLTQS PFKKSKG II MDAKFHPFKP QLFVCSQRYV RIYDLSQQIL VKKLLPGARW LSKIDIHPRG DNLIASSFDK RVLWHDLDLA STPYKTLR Y HEKAVRSVNF HKKLPLFSSA ADDGTIHVFH ATVYDDMMKN PMIVPLKKLT GHKVINSLGV LDAIWHPREA WLFSAGADN TARLWTT

UniProtKB: Ribosome biogenesis protein ERB1

Macromolecule #45: Pescadillo homolog

• Macromolecule: Name: Pescadillo homolog / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 69.984148 KDa

Sequence

String:

MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARK LTRALGRGEV SSAKRLEENR DSYTLDHIIK ERYPSFPDAI RDIDDALNML FLFSNLPSTN QVSSKIINDA Q KICNQWLA YVAKERLVRK VFVSIKGVYY QANIKGEEVR WLVPFKFPEN IPSDVDFRIM LTFLEFYSTL LHFVLYKLYT DS GLIYPPK LDLKKDKIIS GLSSYILESR QEDSLLKLDP TEIEEDVKVE SLDASTLKSA LNADEANTDE TEKEEEQEKK QEK EQEKEQ NEETELDTFE DNNKNKGDIL IQPSKYDSPV ASLFSAFVFY VSREVPIDIL EFLILSCGGN VISEAAMDQI ENKK DIDMS KVTHQIVDRP VLKNKVAGRT YIQPQWIFDC INKGELVPAN KYLPGEALPP HLSPWGDAIG YDPTAPVEEG EEEES ESES ESEDQVEEED QEVVAGEEDD DDDEELQAQK ELELEAQGIK YSETSEADKD VNKSKNKKRK VDEEEEEKKL KMIMMS NKQ KKLYKKMKYS NAKKEEQAEN LKKKKKQIAK QKAKLNKLDS KK

UniProtKB: Pescadillo homolog

Macromolecule #46: Ribosome biogenesis protein 15

• Macromolecule: Name: Ribosome biogenesis protein 15 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 25.499186 KDa

Sequence

String:

MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEY SGIIYVSRLP HGFHEKELSK YFAQFGDLKE VRLARNKKTG NSRHYGFLEF VNKEDAMIAQ ESMNNYLLMG H LLQVRVLP KGAKIEKLYK YKKRVLVEKG ITKPVKQLKD NMKQKHEERI KKLAKSGIEF KW

UniProtKB: Ribosome biogenesis protein 15

Macromolecule #47: YTM1 isoform 1

• Macromolecule: Name: YTM1 isoform 1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 51.426637 KDa

Sequence

String:

MTEDKSQVKI RFFTREKDEL LHVQDTPMYA PISLKRYGLS EIVNHLLGSE KPVPFDFLIE GELLRTSLHD YLTKKGLSSE ASLNVEYTR AILPPSYLNS FSNEDWVSSL DVGDGSKHII SGSYDGIVRT WDLSGNVQKQ YSGHSGPIRA VKYISNTRLV S AGNDRTLR LWKTKNDDLK LTSQQQAQED DDDEVNIEDG KTLAILEGHK APVVSIDVSD NSRILSASYD NSIGFWSTIY KE MTVVDPL EDINNPNNKI STAARKRRKL TMKDGTIRRR APLSLLESHT APVEQVIFDS TDNTVGYSVS QDHTIKTWDL VTA RCIDTR TTSYSLLSIA QLSTLNLLAC GSSARHITLH DPRVGASSKV TQQQLIGHKN FVSSLDTCPE NEYILCSGSH DGTV KVWDV RSTSPMYTIT REDKSVQKGV NDKVFAVKWA EKVGIISAGQ DKKIQINKGD NIFKN

UniProtKB: Ribosome biogenesis protein YTM1

Macromolecule #48: 25S rRNA (cytosine(2870)-C(5))-methyltransferase

• Macromolecule: Name: 25S rRNA (cytosine(2870)-C(5))-methyltransferase / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO / EC number: 25S rRNA (cytosine2870-C5)-methyltransferase
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 69.912164 KDa

Sequence

String:

MGSRRHKNKQ AAPPTLEEFQ ARKEKKANRK LEKGKRPSTT QGDEVSDRKK KKSKPFKKSR KEEEEVVEED KDLPEVDLEE LSKARKSLF DDEEDDDEAG LVDEELKDEF DLEQEYDYDE DEDNDAHPIF SDDDDEADLE ELNAQNMEAL SKKLDEEEAE E AEEAEMEL VEAENMQPRA DILPTEEQEE MMAQETPNLT STRTRMIEIV KVLENFKTLG AEGRSRGEYV DRLLKDICEY FG YTPFLAE KLFNLFSPAE AMEFFEANEI ARPITIRTNT LKTRRRDLAQ TLVNRGVNLQ PIGSWTKVGL QIFDSQVPIG ATP EYLAGH YILQAASSFL PVIALDPHEN ERILDMAAAP GGKTTYISAM MKNTGCVFAN DANKSRTKSL IANIHRLGCT NTIV CNYDA REFPKVIGGF DRILLDAPCS GTGVIGKDQS VKVSRTEKDF IQIPHLQKQL LLSAIDSVDC NSKHGGVIVY STCSV AVEE DEAVIDYALR KRPNVKLVDT GLAIGKEAFT SYRGKKFHPS VKLARRYYPH TYNVDGFFVA KFQKIGPSSF DDNQAS AKE KETAARKEAL EEGIIHSDFA TFEDEEDDKY IEKSVKNNLL KKGVNPKAKR PSNEK

UniProtKB: 25S rRNA (cytosine(2870)-C(5))-methyltransferase

Macromolecule #49: Ribosome biogenesis protein NSA2

• Macromolecule: Name: Ribosome biogenesis protein NSA2 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 29.786783 KDa

Sequence

String:

MPQNDYIERH IKQHGKRLDH EERKRKREAR ESHKISERAQ KLTGWKGKQF AKKRYAEKVS MRKKIKAHEQ SKVKGSSKPL DTDGDALPT YLLDREQNNT AKAISSSIKQ KRLEKADKFS VPLPKVRGIS EEEMFKVIKT GKSRSKSWKR MITKHTFVGE G FTRRPVKM ERIIRPSALR QKKANVTHPE LGVTVFLPIL AVKKNPQSPM YTQLGVLTKG TIIEVNVSEL GMVTAGGKVV WG KYAQVTN EPDRDGCVNA VLLV

UniProtKB: Ribosome biogenesis protein NSA2

Macromolecule #50: Nuclear GTP-binding protein NUG1

• Macromolecule: Name: Nuclear GTP-binding protein NUG1 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 57.798652 KDa

Sequence

String:

MRVRKRQSRR TSTKLKEGIK KKASAHRKKE KKMAKKDVTW RSRSKKDPGI PSNFPYKAKI LEEIEAKKMK DLEERELAKQ QRLEARKAA KEQGVDAMDE DMIEDDENGL AALVESAQQA AAEYEGTPSN DADVRDDELD VIDYNIDFYG EDVEGESELE K SRKAYDKI FKSVIDASDV ILYVLDARDP ESTRSRKVEE AVLQSQGKRL ILILNKVDLI PPHVLEQWLN YLKSSFPTIP LR ASSGAVN GTSFNRKLSQ TTTASALLES LKTYSNNSNL KRSIVVGVIG YPNVGKSSVI NALLARRGGQ SKACPVGNEA GVT TSLREI KIDNKLKILD SPGICFPSEN KKRSKVEHEA ELALLNALPA KHIVDPYPAV LMLVKRLAKS DEMTESFKKL YEIP PIPAN DADTFTKHFL IHVARKRGRL GKGGIPNLAS AGLSVLNDWR DGKILGWVLP NTSAAASQQD KQNLSTINTG TKQAP IAAN ESTIVSEWSK EFDLDGLFSS LDKAIDASKD QDTMME

UniProtKB: Nuclear GTP-binding protein NUG1

Macromolecule #51: Ribosome biogenesis protein RLP7

• Macromolecule: Name: Ribosome biogenesis protein RLP7 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 36.621074 KDa

Sequence

String:

MSSTQDSKAQ TLNSNPEILL RKRRNADRTR IERQELAKKK REEQIKKKRS NKNKFVRAES IVAKTLATSR EKERIKRVSI LEDKKAKNE TQHIASGKDF ILKITEKANG AEENSVDLEE TEEEEDDGLI REKTTYDGKP ALLFIVRVRG PLAVNIPNKA F KILSLLRL VETNTGVFVK LTKNVYPLLK VIAPYVVIGK PSLSSIRSLI QKRGRIIYKG ENEAEPHEIV LNDNNIVEEQ LG DHGIICV EDIIHEIATM GESFSVCNFF LQPFKLNREV SGFGSLNRLR KIKQREAESR TRQFSNAATA PVIEVDIDSL LAK LN

UniProtKB: Ribosome biogenesis protein RLP7

Macromolecule #52: Ribosome biogenesis protein RLP24

• Macromolecule: Name: Ribosome biogenesis protein RLP24 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 24.02765 KDa

Sequence

String:

MRIYQCHFCS SPCYPGHGIM FVRNDAKEFR FCRSKCHKAF KQRRNPRKLK WTKAFRKAAG KELAVDSTLT FAQRRNVPVR YNRELVATT LKAMARIEEI RQKRERAFYK NRMRGNKEKD FLRDKKLVES NPELLRIREV EIARKLAKEQ ERAESVSEQE E SEEEEEDM EIDSDEEEEE QLEKQKILLK NRRRNTKKIA F

UniProtKB: Ribosome biogenesis protein RLP24

Macromolecule #53: Nucleolar protein 16

• Macromolecule: Name: Nucleolar protein 16 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 26.954447 KDa

Sequence

String:

MTSVRKRKMN RSSVGKATRR NKDKQRKINI QSNPIIAANW DYSLTMAQNY KKLGLRAKLQ TPAGGKEADL SKVVKRIPLT KPVLDEDED EDEGEDEQND YNAATVELDE NEIPEGGARI QRDKNGDVVR VVYGKKKNFD ADEDVNEIKA RDTTEETEVV K KLEELASR PVIRKERSQS EREEEWLEKL YKKHGDDYKK MFFDKKLNIY QQSEGDLKRR LLRWKKRNGI ASK

UniProtKB: Nucleolar protein 16

Macromolecule #54: 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase

• Macromolecule: Name: 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO; EC number: 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 96.656172 KDa

Sequence

String:

MGKTQKKNSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV IDLCAAPGSW CQVASKLCPV NSLIIGVDIV PMKPMPNVI TFQSDITTED CRSKLRGYMK TWKADTVLHD GAPNVGLGWV QDAFTQSQLT LQALKLAVEN LVVNGTFVTK I FRSKDYNK LIWVFQQLFE KVEATKPPAS RNVSAEIFVV CKGFKAPKRL DPRLLDPKEV FEELPDGQQN MESKIYNPEK KV RKRQGYE EGDNLLYHET SILDFVRTED PISMLGEMNK FTIDENDHEW KILKKLKQTT DEFRSCIEDL KVLGKKDFKM ILR WRKIAR EILGIEVKDD AKTEIEVVPL TEEEQIEKDL QGLQEKQRLN VKRERRRKNE MKQKELQRMQ MNMITPTDIG IEAA SLGKE SLFNLKTAEK TGILNDLAKG KKRMIFTDDE LAKDNDIYID ENIMIKDKDS AADADDLESE LNAMYSDYKT RRSER DAKF RAKQARGGDN EEEWTGFNEG SLEKKEEEGK DYIEDNDDEG VEGDSDDDEA ITNLISKLKG QEGDHKLSSK ARMIFN DPI FNNVEPDLPV NTVNDGIMSS ESVGDISKLN KKRKHEEMHQ KQDEADSSDE SSSDDSDFEI VANDNASEEF DSDYDSE EE KNQTKKEKHS RDIDIATVEA MTLAHQLALG QKNKHDLVDE GFNRYTFRDT ENLPDWFLED EKEHSKINKP ITKEAAMA I KEKIKAMNAR PIKKVAEAKA RKRMRAVARL EKIKKKAGLI NDDSDKTEKD KAEEISRLMR KVTKKPKTKP KVTLVVASG RNKGLAGRPK GVKGKYKMVD GVMKNEQRAL RRIAKKHHKK K

UniProtKB: 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase

Macromolecule #55: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 26.476605 KDa

Sequence

String:

MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ DQEELSSLLQ VPLVAGTVNR GSSVVGAGMV VNDYLAVTGL DTTAPELSVI ESIFRLQDAQ PESISGNLRD TL IETYS

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #56: UPF0642 protein YBL028C

• Macromolecule: Name: UPF0642 protein YBL028C / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae BY4741 (yeast)
• Molecular weight: Theoretical: 12.435429 KDa

Sequence

String:

MAKSLRASSH LNAKSVKRRG VFQKAVDARE QRISDKLKED LLKQKLEDLK KKEEQGIDMD VDEKKSNEEA PRKKISTSGW RDGRHHTYK KAKLMKQSKK KTSFTRF

UniProtKB: UPF0642 protein YBL028C

Macromolecule #57: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 57 / Number of copies: 1 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Macromolecule #58: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 2 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #59: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 59 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #60: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 60 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #61: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 61 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.5 mg/mL

Buffer

pH: 8
Component:

Concentration	Name	Formula
50.0 mM	Bis-Tris-Propane
125.0 mM	Sodium Chloride	NaCl
25.0 mM	Potassium Chloride	KCl
10.0 mM	Magnesium Chloride	MgCl2

• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 39.3 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.9 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1202000

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

6em1

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.66 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0.1) / Number images used: 19700
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0.1)