EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic insights into the alternative ribosome recycling by HflXr.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 52, Issue 7, Page 4053-4066, Year 2024
掲載日	2024年4月24日
著者	Savannah M Seely / Ritwika S Basu / Matthieu G Gagnon /
PubMed 要旨	During stress conditions such as heat shock and antibiotic exposure, ribosomes stall on messenger RNAs, leading to inhibition of protein synthesis. To remobilize ribosomes, bacteria use rescue ...During stress conditions such as heat shock and antibiotic exposure, ribosomes stall on messenger RNAs, leading to inhibition of protein synthesis. To remobilize ribosomes, bacteria use rescue factors such as HflXr, a homolog of the conserved housekeeping GTPase HflX that catalyzes the dissociation of translationally inactive ribosomes into individual subunits. Here we use time-resolved cryo-electron microscopy to elucidate the mechanism of ribosome recycling by Listeria monocytogenes HflXr. Within the 70S ribosome, HflXr displaces helix H69 of the 50S subunit and induces long-range movements of the platform domain of the 30S subunit, disrupting inter-subunit bridges B2b, B2c, B4, B7a and B7b. Our findings unveil a unique ribosome recycling strategy by HflXr which is distinct from that mediated by RRF and EF-G. The resemblance between HflXr and housekeeping HflX suggests that the alternative ribosome recycling mechanism reported here is universal in the prokaryotic kingdom.
リンク	Nucleic Acids Res / PubMed:38407413 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.8 Å
構造データ	42554 8uu4 EMDB-42554, PDB-8uu4: Cryo-EM structure of the Listeria innocua 70S ribosome in complex with HPF (structure I-A) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-42555: Refined map of the Listeria innocua 70S ribosome (head-swiveled) in complex with pe/E-tRNA (structure I-B) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-42556: Focus refined map of the swiveled head domain of the 30S subunit of Listeria innocua ribosome (structure I-B) 手法: EM (単粒子) / 解像度: 3.0 Å 42557 8uu5 EMDB-42557, PDB-8uu5: Cryo-EM structure of the Listeria innocua 70S ribosome (head-swiveled) in complex with pe/E-tRNA (structure I-B) 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-42558: Refined map of the ratcheted Listeria innocua 70S ribosome in complex with p/E-tRNA (structure II-A) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-42559: Focus refined map of the head domain of the ratcheted 30S subunit of Listeria innocua ribosome (structure II-A) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-42560: Focus refined map of the body domain of the ratcheted 30S subunit of Listeria innocua ribosome (structure II-A) 手法: EM (単粒子) / 解像度: 3.2 Å 42561 8uu6 EMDB-42561, PDB-8uu6: Cryo-EM structure of the ratcheted Listeria innocua 70S ribosome in complex with p/E-tRNA (structure II-A) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-42562: Refined map of the Listeria innocua 70S ribosome in complex with HflXr, HPF, and E-site tRNA (structure II-B) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-42563: Focus refined map of the head domain of the 30S subunit of Listeria innocua ribosome in complex with HflXr, HPF, and E-site tRNA (structure II-B) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-42564: Focus refined map of the body domain of the 30S subunit of Listeria innocua ribosome in complex with HflXr, HPF, and E-site tRNA (structure II-B) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-42565: Focus refined map of HflXr bound to the Listeria innocua ribosome in complex with HPF and E-site tRNA (structure II-B) 手法: EM (単粒子) / 解像度: 3.8 Å 42566 8uu7 EMDB-42566, PDB-8uu7: Cryo-EM structure of the Listeria innocua 70S ribosome in complex with HflXr, HPF, and E-site tRNA (structure II-B) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-42567: Refined map of the Listeria innocua 70S ribosome (head-swiveled) in complex with HflXr and pe/E-tRNA (structure II-C) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-42568: Focus refined map of the swiveled head domain of the 30S subunit of Listeria innocua ribosome in complex with HflXr and pe/E-tRNA (structure II-C) 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-42569: Focus refined map of the body domain of the 30S subunit of Listeria innocua ribosome in complex with HflXr and pe/E-tRNA (structure II-C) 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-42570: Focus refined map of HflXr bound to the Listeria innocua ribosome in complex with pe/E-tRNA (structure II-C) 手法: EM (単粒子) / 解像度: 3.0 Å 42571 8uu8 EMDB-42571, PDB-8uu8: Cryo-EM structure of the Listeria innocua 70S ribosome (head-swiveled) in complex with HflXr and pe/E-tRNA (structure II-C) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-42572: Refined map of the ratcheted Listeria innocua 70S ribosome (head-swiveled) in complex with HflXr and pe/E-tRNA (structure II-D) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-42573: Focus refined map of the swiveled head domain of the 30S subunit of ratcheted Listeria innocua ribosome in complex with HflXr and pe/E-tRNA (structure II-D) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-42574: Focus refined map of the body domain of the 30S subunit of ratcheted Listeria innocua ribosome in complex with HflXr and pe/E-tRNA (structure II-D) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-42575: Focus refined map of HflXr bound to the ratcheted Listeria innocua ribosome in complex with pe/E-tRNA (structure II-D) 手法: EM (単粒子) / 解像度: 3.0 Å 42576 8uu9 EMDB-42576, PDB-8uu9: Cryo-EM structure of the ratcheted Listeria innocua 70S ribosome (head-swiveled) in complex with HflXr and pe/E-tRNA (structure II-D) 手法: EM (単粒子) / 解像度: 3.1 Å 42577 8uua EMDB-42577, PDB-8uua: Cryo-EM structure of the Listeria innocua 50S ribosomal subunit in complex with HflXr (structure III) 手法: EM (単粒子) / 解像度: 2.7 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-K: Unknown entry / カリウムカチオン ChemComp-GCP: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / グアニリルメチレンジホスホン酸 / GMP-PCP, エネルギー貯蔵分子類似体*YM
由来	listeria innocua (バクテリア) escherichia coli (大腸菌) escherichia phage t4 (ファージ) listeria monocytogenes egd-e (バクテリア) listeria monocytogenes serovar 1/2a (strain atcc baa-679 / egd-e) (バクテリア)
キーワード	RIBOSOME / cryo-EM / recycling / HPF / time-resolved / HflXr